Fluorescence loss in photobleaching, the Glossary
Fluorescence Loss in Photobleaching (FLIP) is a fluorescence microscopy technique used to examine movement of molecules inside cells and membranes.[1]
Table of Contents
7 relations: Confocal microscopy, Fluorescence microscope, Fluorescence recovery after photobleaching, Fluorophore, Green fluorescent protein, Photobleaching, Springer Science+Business Media.
- Fluorescence techniques
Confocal microscopy
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Fluorescence loss in photobleaching and confocal microscopy are fluorescence techniques.
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Fluorescence microscope
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances.
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Fluorescence recovery after photobleaching
Fluorescence recovery after photobleaching (FRAP) is a method for determining the kinetics of diffusion through tissue or cells. Fluorescence loss in photobleaching and Fluorescence recovery after photobleaching are fluorescence techniques.
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Fluorophore
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation.
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Green fluorescent protein
The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range.
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Photobleaching
In optics, photobleaching (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce.
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Springer Science+Business Media, commonly known as Springer, is a German multinational publishing company of books, e-books and peer-reviewed journals in science, humanities, technical and medical (STM) publishing.
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See also
Fluorescence techniques
- Chemical imaging
- Cold vapour atomic fluorescence spectroscopy
- Confocal microscopy
- Förster resonance energy transfer
- Fluorescence anisotropy
- Fluorescence biomodulation
- Fluorescence correlation spectroscopy
- Fluorescence cross-correlation spectroscopy
- Fluorescence image-guided surgery
- Fluorescence imaging
- Fluorescence intensity decay shape microscopy
- Fluorescence loss in photobleaching
- Fluorescence recovery after photobleaching
- Fluorescence-lifetime imaging microscopy
- Fluorescent tag
- Kautsky effect
- Lattice light-sheet microscopy
- Light sheet fluorescence microscopy
- Light-induced fluorescence transient
- Multifocal plane microscopy
- PGLO
- Super-resolution microscopy
- Supercritical angle fluorescence microscopy
- Three-photon microscopy
- Time-resolved fluorescence energy transfer
- Total internal reflection fluorescence microscope
- Two-photon excitation microscopy
References
[1] https://en.wikipedia.org/wiki/Fluorescence_loss_in_photobleaching
Also known as FLIP (Fluorescence-Loss-In-Photobleaching).