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GAL4/UAS system, the Glossary

The GAL4-UAS system is a biochemical method used to study gene expression and function in organisms such as the fruit fly.^[1]

33 relations: Activator (genetics), Andrea Brand, Biochemistry, Calcium, Channelrhodopsin, Circadian rhythm, DNA-binding domain, Drosophila melanogaster, Ectotherm, Fluorescence microscope, Gal4 transcription factor, GCaMP, Gene expression, Green fluorescent protein, Halorhodopsin, Leucine zipper, Nature (journal), Neuron, Norbert Perrimon, Nucleotide base, Pigment dispersing factor, Proceedings of the National Academy of Sciences of the United States of America, Promoter (genetics), Protein, Protein domain, Red fluorescent protein, Reporter gene, Saccharomyces cerevisiae, Transcription (biology), Two-hybrid screening, Upstream activating sequence, Xenopus, Zebrafish.

Activator (genetics)

A transcriptional activator is a protein (transcription factor) that increases transcription of a gene or set of genes.

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Andrea Brand

Andrea Hilary Brand (born March 9, 1959) is the Herchel Smith Professor of Molecular Biology and a Fellow of Jesus College, Cambridge.

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Biochemistry

Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms.

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Calcium

Calcium is a chemical element; it has symbol Ca and atomic number 20.

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Channelrhodopsin

Channelrhodopsins are a subfamily of retinylidene proteins (rhodopsins) that function as light-gated ion channels.

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Circadian rhythm

A circadian rhythm, or circadian cycle, is a natural oscillation that repeats roughly every 24 hours.

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DNA-binding domain

A DNA-binding domain (DBD) is an independently folded protein domain that contains at least one structural motif that recognizes double- or single-stranded DNA.

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Drosophila melanogaster

Drosophila melanogaster is a species of fly (an insect of the order Diptera) in the family Drosophilidae.

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An ectotherm (from the Greek ἐκτός "outside" and θερμός "heat"), more commonly referred to as a "cold-blooded animal", is an animal in which internal physiological sources of heat, such as blood, are of relatively small or of quite negligible importance in controlling body temperature.

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Fluorescence microscope

A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances.

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Gal4 transcription factor

The Gal4 transcription factor is a positive regulator of gene expression of galactose-induced genes.

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GCaMP

GCaMP is a genetically encoded calcium indicator (GECI) initially developed in 2001 by Junichi Nakai.

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Gene expression

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, proteins or non-coding RNA, and ultimately affect a phenotype.

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Green fluorescent protein

The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range.

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Halorhodopsin

Halorhodopsin is a seven-transmembrane retinylidene protein from microbial rhodopsin family.

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Leucine zipper

A leucine zipper (or leucine scissors) is a common three-dimensional structural motif in proteins.

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Nature (journal)

Nature is a British weekly scientific journal founded and based in London, England.

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Neuron

A neuron, neurone, or nerve cell is an excitable cell that fires electric signals called action potentials across a neural network in the nervous system.

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Norbert Perrimon

Norbert Perrimon is a French geneticist and developmental biologist.

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Nucleotide base

Nucleotide bases (also nucleobases, nitrogenous bases) are nitrogen-containing biological compounds that form nucleosides, which, in turn, are components of nucleotides, with all of these monomers constituting the basic building blocks of nucleic acids.

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Pigment dispersing factor

Pigment dispersing factor (pdf) is a gene that encodes the protein PDF, which is part of a large family of neuropeptides.

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Proceedings of the National Academy of Sciences of the United States of America

Proceedings of the National Academy of Sciences of the United States of America (often abbreviated PNAS or PNAS USA) is a peer-reviewed multidisciplinary scientific journal.

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In genetics, a promoter is a sequence of DNA to which proteins bind to initiate transcription of a single RNA transcript from the DNA downstream of the promoter.

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Protein

Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues.

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Protein domain

In molecular biology, a protein domain is a region of a protein's polypeptide chain that is self-stabilizing and that folds independently from the rest.

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Red fluorescent protein

Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited.

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Reporter gene

In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants.

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Saccharomyces cerevisiae

Saccharomyces cerevisiae (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungal microorganisms).

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Transcription (biology)

Transcription is the process of copying a segment of DNA into RNA.

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Two-hybrid screening

Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.

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References

[1] https://en.wikipedia.org/wiki/GAL4/UAS_system

Also known as Gal4-UAS.

GAL4/UAS system, the Glossary

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References