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Suspension culture, the Glossary

Index Suspension culture

A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension.[1]

Table of Contents

  1. 40 relations: Adherent culture, Aedes albopictus, Alexis Carrel, Bio-MEMS, Cabbage looper, Cell (biology), Cell adhesion, Cell adhesion molecule, Cell culture, Cell division, Cell physiology, Chinese hamster ovary cell, Drosophila melanogaster, Eagle's minimal essential medium, Fall armyworm, George Otto Gey, Growth medium, Haematopoiesis, HeLa, Hemocytometer, High Five cells, Homogenization (biology), Insect cell culture, Jurkat cells, Leonard Hayflick, Montrose Thomas Burrows, Morphology (biology), Plant cell, Primary cell culture, Ross Granville Harrison, Schneider 2 cells, Senescence, Sf21, Sf9 (cells), Shear force, Surfactant, Suspension (chemistry), Trypan blue, White blood cell, Wilhelm Roux.

  2. Cell culture techniques

Adherent culture

Adherent cell cultures are a type of cell culture that requires cells to be attached to a surface in order for growth to occur.

See Suspension culture and Adherent culture

Aedes albopictus

Aedes albopictus (synonym Stegomyia albopicta), from the mosquito (Culicidae) family, also known as the (Asian) tiger mosquito or forest mosquito, is a mosquito native to the tropical and subtropical areas of Southeast Asia.

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Alexis Carrel

Alexis Carrel (28 June 1873 – 5 November 1944) was a French surgeon and biologist who spent most of his scientific career in the United States.

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Bio-MEMS

Bio-MEMS is an abbreviation for biomedical (or biological) microelectromechanical systems.

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Cabbage looper

The cabbage looper (Trichoplusia ni) is a medium-sized moth in the family Noctuidae, a family commonly referred to as owlet moths.

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Cell (biology)

The cell is the basic structural and functional unit of all forms of life. Suspension culture and cell (biology) are cell biology.

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Cell adhesion

Cell adhesion is the process by which cells interact and attach to neighbouring cells through specialised molecules of the cell surface. Suspension culture and cell adhesion are cell biology.

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Cell adhesion molecule

Cell adhesion molecules (CAMs) are a subset of cell surface proteins that are involved in the binding of cells with other cells or with the extracellular matrix (ECM), in a process called cell adhesion.

See Suspension culture and Cell adhesion molecule

Cell culture

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. Suspension culture and cell culture are cell biology.

See Suspension culture and Cell culture

Cell division

Cell division is the process by which a parent cell divides into two daughter cells.

See Suspension culture and Cell division

Cell physiology

Cell physiology is the biological study of the activities that take place in a cell to keep it alive. Suspension culture and cell physiology are cell biology.

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Chinese hamster ovary cell

Chinese hamster ovary (CHO) cells are a family of immortalized cell lines derived from epithelial cells of the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of recombinant therapeutic proteins.

See Suspension culture and Chinese hamster ovary cell

Drosophila melanogaster

Drosophila melanogaster is a species of fly (an insect of the order Diptera) in the family Drosophilidae.

See Suspension culture and Drosophila melanogaster

Eagle's minimal essential medium

Minimal essential medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in ''Science'' that can be used to maintain cells in tissue culture.

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Fall armyworm

The fall armyworm (Spodoptera frugiperda) is a species in the order Lepidoptera and one of the species of the fall armyworm moths distinguished by their larval life stage.

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George Otto Gey

George Otto Gey (July 6, 1899 – November 8, 1970) was the cell biologist at Johns Hopkins Hospital who is credited with propagating the HeLa cell line from Henrietta Lacks' cervical tumor.

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Growth medium

A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss Physcomitrella patens.

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Haematopoiesis

Haematopoiesis (from Greek αἷμα, 'blood' and ποιεῖν 'to make'; also hematopoiesis in American English; sometimes also h(a)emopoiesis) is the formation of blood cellular components.

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HeLa

HeLa is an immortalized cell line used in scientific research.

See Suspension culture and HeLa

Hemocytometer

The hemocytometer (or haemocytometer) is a counting-chamber device originally designed and usually used for counting blood cells.

See Suspension culture and Hemocytometer

High Five cells

High Five (BTI-Tn-5B1-4) is an insect cell line that originated from the ovarian cells of the cabbage looper, Trichoplusia ni. It was developed by the Boyce Thompson Institute for Plant Research.

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Homogenization (biology)

Homogenization, in cell biology or molecular biology, is a process whereby different fractions of a biological sample become equal in composition. Suspension culture and Homogenization (biology) are cell biology.

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Insect cell culture

The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. Suspension culture and insect cell culture are cell culture techniques.

See Suspension culture and Insect cell culture

Jurkat cells

Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.

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Leonard Hayflick

Leonard Hayflick (born 20 May 1928) is a Professor of Anatomy at the UCSF School of Medicine, and was Professor of Medical Microbiology at Stanford University School of Medicine.

See Suspension culture and Leonard Hayflick

Montrose Thomas Burrows

Montrose Thomas Burrows (1884 – 1947) was a US surgeon and pathologist specializing in cancer research and surgery.

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Morphology (biology)

Morphology in biology is the study of the form and structure of organisms and their specific structural features.

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Plant cell

Plant cells are the cells present in green plants, photosynthetic eukaryotes of the kingdom Plantae.

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Primary cell culture

Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.

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Ross Granville Harrison

Ross Granville Harrison (January 13, 1870 – September 30, 1959) was an American biologist and anatomist credited for his pioneering work on animal tissue culture.

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Schneider 2 cells

Schneider 2 cells, usually abbreviated as S2 cells, are one of the most commonly used Drosophila melanogaster cell lines.

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Senescence

Senescence or biological aging is the gradual deterioration of functional characteristics in living organisms.

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Sf21

Sf21 (officially called IPLB-Sf21-AE) is a continuous cell line developed from ovaries of the Fall Army worm, Spodoptera frugiperda, a moth species that is an agricultural pest on corn and other grass species.

See Suspension culture and Sf21

Sf9 (cells)

Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus.

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Shear force

In solid mechanics, shearing forces are unaligned forces acting on one part of a body in a specific direction, and another part of the body in the opposite direction.

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Surfactant

Surfactants are chemical compounds that decrease the surface tension or interfacial tension between two liquids, a liquid and a gas, or a liquid and a solid.

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Suspension (chemistry)

In chemistry, a suspension is a heterogeneous mixture of a fluid that contains solid particles sufficiently large for sedimentation.

See Suspension culture and Suspension (chemistry)

Trypan blue

Trypan blue is an azo dye.

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White blood cell

White blood cells (scientific name leukocytes), also called immune cells or immunocytes, are cells of the immune system that are involved in protecting the body against both infectious disease and foreign invaders.

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Wilhelm Roux

Wilhelm Roux (9 June 1850 – 15 September 1924) was a German zoologist and pioneer of experimental embryology.

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See also

Cell culture techniques

References

[1] https://en.wikipedia.org/wiki/Suspension_culture

Also known as Cell suspension, Cell suspension culture.