Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1
- ️Scheres, S. H. W.
- ️Tue Feb 11 2020
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Figure 6
Half-set (top) and map versus atomic model (bottom) FSC plots for the phase-plate data set. The atomic model used was again PDB entry 6bdf. Note that considering the aberrations does not improve the resolution, since these types of aberrations cannot be expressed by our model. Nevertheless, the CTF refinement does improve the resolution owing to the new micrograph global defocus and phase-shift estimation and owing to considering the slightly anisotropic magnification. |
ISSN: 2052-2525
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