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Patchy field defects of apoptosis resistance and dedifferentiation in flat mucosa of colon resections from colon cancer patients - Annals of Surgical Oncology

  • ️Bernstein, Carol
  • ️Sat Jun 01 2002

Abstract

Background

Abnormal areas in normal-appearing falt colonic mucosa (field defects) may predispose individuals to colon cancer. Markers of field defects would indicate cancer risk.

Methods

We evaluated apoptosis capability, dedifferentiation, frequency of simple aberrant crypts, aberrant crypt foci, microadenomas, and total nicotinamide adenine dinucleotide levels at locations with normal-appearing flat mucosa obtained from colon resections.

Results

Among goblet cells from colonic mucosa samples of individuals without colonic neoplasia, there was a high mean deoxycholate-induced apoptotic index (AI) of 59.1% and highDolichos biflorus agglutinin (DBA) lectin reactivity (differntiation) in 85.0% of samples. In contrast, flat mucosa samples from colon cancer patients had a significantly (P<.01) lower average AI of 37.4%, and a significantly (P=.03) lower percentage (40.5%) had high DBA reactivity. For colon cancer patients, AI and DBA reactivity values were patchy within a resection. Nicotinamide adenine dinucleotide levels were highly variable among individuals without neoplasia, and aberrant crypt focia and microadenomas were rare.

Conclusions

AI and aberrant DBA reactivity are promising indicators of colon cancer risk. Our results attest to the importance of obtaining multiple samples to assess colon cancer risk because of the patchy nature of field defects.

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Authors and Affiliations

  1. Department of Surgery, University of Arizona, Tucson, Arizona

    James A. Warneke MD

  2. Department of Medicine, University of Arizona, Tucson, Arizona

    Harinder Garewal MD, PhD & David L. Earnest MD

  3. Department of Pharmacology and Toxicology, University of Arizona, Tucson, Arizona

    Elaine L. Jacobson PhD

  4. Department of College of Public Health, University of Arizona, Tucson, Arizona

    Denise J. Roe PhD

  5. Department of the Arizona Cancer Center, University of Arizona, Tucson, Arizona

    Harris Bernstein PhD, James A. Warneke MD, Harinder Garewal MD, PhD, David L. Earnest MD, Claire M. Payne PhD, Denise J. Roe PhD, Haiyan Cui PhD & Elaine L. Jacobson PhD

  6. Tucson Veterans Affairs Hospital, Tucson, Arizona

    Harinder Garewal MD, PhD

  7. Department of Cancer Center, University of Kentucky, Lexington, Kentucky

    Elaine L. Jacobson PhD

  8. Department of Microbiology and Immunology, College of Medicine, University of Arizona, 85724, Tucson, AZ

    Harris Bernstein PhD, Hana Holubec MS, Claire M. Payne PhD & Carol Bernstein PhD

Authors

  1. Harris Bernstein PhD

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  2. Hana Holubec MS

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  3. James A. Warneke MD

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  4. Harinder Garewal MD, PhD

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  5. David L. Earnest MD

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  6. Claire M. Payne PhD

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  7. Denise J. Roe PhD

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  8. Haiyan Cui PhD

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  9. Elaine L. Jacobson PhD

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  10. Carol Bernstein PhD

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Correspondence to Carol Bernstein PhD.

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Bernstein, H., Holubec, H., Warneke, J.A. et al. Patchy field defects of apoptosis resistance and dedifferentiation in flat mucosa of colon resections from colon cancer patients. Annals of Surgical Oncology 9, 505–517 (2002). https://doi.org/10.1007/BF02557276

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  • Received: 07 November 2001

  • Accepted: 27 February 2002

  • Issue Date: June 2002

  • DOI: https://doi.org/10.1007/BF02557276

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