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Application of embryo rescue after interspecific crosses in the genus Rhododendron - Plant Cell, Tissue and Organ Culture (PCTOC)

  • ️Van Bockstaele, Erik
  • ️Tue Feb 20 2007

Abstract

Interspecific hybridization between evergreen pot azalea (Tsutsusi) cultivars and genotypes of other Rhododendron subgenera or sections (Rhododendron, Hymenanthes, Pentanthera, Vireya) is significantly hampered by many prezygotic and postzygotic barriers. The objective of our work was to overcome spontaneous abortion and lack of endosperm formation and to increase germination rates by establishing an embryo rescue protocol. The optimal germination medium for immature Rhododendron seeds was a basal medium supplemented with 145 μM GA3. This medium induced germination of fertilized ovules after several sexual combinations of subgenera. Its use was clearly more efficient than in vivo sowing. The direction of the cross significantly influenced the occurrence of abortion, germination and albinism. The obtained seedlings were multiplied on Woody Plant Medium + 4.5 μM 2iP, and rooted afterwards. Finally, about 9% of the germinated ovules resulted in vigorously growing seedlings that were successfully acclimatized.

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Abbreviations

2iP:

Isopentenyladenine

GA:

Gibberellic acid

HY:

Subgenus Hymenanthes

IAA:

Indole acetic acid

MS:

Murashige & Skoog

PE:

Subgenus Pentanthera

RH:

Subgenus Rhododendron

TS:

Subgenus Tsutsusi

VI:

Section Vireya

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Acknowledgements

This work was financed by DWTC-Belgium. The authors wish to thank Romain Uytterhaegen, Ronald Van den Oord, Kristien Janssens, Veerle Cools and Laurence Desmet for technical assistance.

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Authors and Affiliations

  1. Unit Plant – Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium

    Tom Eeckhaut, Ellen De Keyser, Johan Van Huylenbroeck, Jan De Riek & Erik Van Bockstaele

  2. Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000, Gent, Belgium

    Tom Eeckhaut & Erik Van Bockstaele

Authors

  1. Tom Eeckhaut

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  2. Ellen De Keyser

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  3. Johan Van Huylenbroeck

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  4. Jan De Riek

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  5. Erik Van Bockstaele

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Corresponding author

Correspondence to Tom Eeckhaut.

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Eeckhaut, T., De Keyser, E., Van Huylenbroeck, J. et al. Application of embryo rescue after interspecific crosses in the genus Rhododendron . Plant Cell Tiss Organ Cult 89, 29–35 (2007). https://doi.org/10.1007/s11240-007-9209-4

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  • Received: 01 November 2006

  • Accepted: 17 January 2007

  • Published: 20 February 2007

  • Issue Date: April 2007

  • DOI: https://doi.org/10.1007/s11240-007-9209-4

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