Application of embryo rescue after interspecific crosses in the genus Rhododendron - Plant Cell, Tissue and Organ Culture (PCTOC)
- ️Van Bockstaele, Erik
- ️Tue Feb 20 2007
Abstract
Interspecific hybridization between evergreen pot azalea (Tsutsusi) cultivars and genotypes of other Rhododendron subgenera or sections (Rhododendron, Hymenanthes, Pentanthera, Vireya) is significantly hampered by many prezygotic and postzygotic barriers. The objective of our work was to overcome spontaneous abortion and lack of endosperm formation and to increase germination rates by establishing an embryo rescue protocol. The optimal germination medium for immature Rhododendron seeds was a basal medium supplemented with 145 μM GA3. This medium induced germination of fertilized ovules after several sexual combinations of subgenera. Its use was clearly more efficient than in vivo sowing. The direction of the cross significantly influenced the occurrence of abortion, germination and albinism. The obtained seedlings were multiplied on Woody Plant Medium + 4.5 μM 2iP, and rooted afterwards. Finally, about 9% of the germinated ovules resulted in vigorously growing seedlings that were successfully acclimatized.
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Abbreviations
- 2iP:
-
Isopentenyladenine
GA: -
Gibberellic acid
HY: -
Subgenus Hymenanthes
IAA: -
Indole acetic acid
MS: -
Murashige & Skoog
PE: -
Subgenus Pentanthera
RH: -
Subgenus Rhododendron
TS: -
Subgenus Tsutsusi
VI: -
Section Vireya
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Acknowledgements
This work was financed by DWTC-Belgium. The authors wish to thank Romain Uytterhaegen, Ronald Van den Oord, Kristien Janssens, Veerle Cools and Laurence Desmet for technical assistance.
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Authors and Affiliations
Unit Plant – Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium
Tom Eeckhaut, Ellen De Keyser, Johan Van Huylenbroeck, Jan De Riek & Erik Van Bockstaele
Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000, Gent, Belgium
Tom Eeckhaut & Erik Van Bockstaele
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- Tom Eeckhaut
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- Ellen De Keyser
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- Johan Van Huylenbroeck
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- Jan De Riek
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- Erik Van Bockstaele
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Correspondence to Tom Eeckhaut.
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Eeckhaut, T., De Keyser, E., Van Huylenbroeck, J. et al. Application of embryo rescue after interspecific crosses in the genus Rhododendron . Plant Cell Tiss Organ Cult 89, 29–35 (2007). https://doi.org/10.1007/s11240-007-9209-4
Received: 01 November 2006
Accepted: 17 January 2007
Published: 20 February 2007
Issue Date: April 2007
DOI: https://doi.org/10.1007/s11240-007-9209-4