CN102960341B - Pesticide composition for relieving phytotoxicity of herbicide - Google Patents

Disclosure of Invention

The invention aims to provide a pesticide composition for relieving phytotoxicity of herbicides, which has the advantages of high efficiency, low toxicity, no environmental pollution and wide mixing range.

The invention provides a pesticide composition for relieving herbicide hazard, which comprises the effective components of a plant growth regulator and active polysaccharide.

Specifically, the effective components of the pesticide composition consist of the following components in parts by weight: 0.05-90.0 parts of plant growth regulator and 0.05-90.0 parts of active polysaccharide.

Preferably, the effective components of the pesticide composition consist of the following components in parts by weight: 0.4-40.0 parts of plant growth regulator and 0.4-40.0 parts of active polysaccharide.

Further preferably, the active ingredients of the pesticide composition consist of the following components in parts by weight: 2.0-40.0 parts of plant growth regulator and 0.4-40.0 parts of active polysaccharide.

Wherein,

the weight portion of the pesticide composition can be the known weight unit in the pesticide field such as mu g, mg, g, kg, and the like, and can also be the multiple of the known weight unit, such as 10 times, 100 times, one tenth, one hundredth.

The plant growth regulator is one or a mixture of compound nitrophenolate and diethyl aminoethyl hexanoate, wherein the compound nitrophenolate is compound sodium nitrophenolate, compound potassium nitrophenolate or compound ammonium nitrophenolate, and the compound nitrophenolate is a compound mixture of 5-nitroguaiacol salt, o-nitrophenolate and p-nitrophenolate in a weight ratio of 1: 2: 3, and the sodium salt, the potassium salt and the ammonium salt are similar bioactive substances.

The active polysaccharide is any one of chitosan oligosaccharide and glucan or a mixture of the chitosan oligosaccharide and the glucan.

The pesticide composition for relieving the phytotoxicity of the herbicide also contains 1.0 to 99.0 parts by weight of soluble fillers, wherein the soluble fillers are various soluble fillers commonly used in the field, and according to the common general knowledge of the technical personnel in the field, inorganic salts or organic matters containing plant nutrient components, such as inorganic salts or organic salts containing potassium, phosphorus, magnesium, calcium, manganese, copper, zinc, ammonium and the like, urea containing nitrogen and the like are preferred, and the soluble fillers are specifically calcium chloride, potassium chloride, manganese chloride, magnesium chloride, ammonium chloride, urea, ferrous sulfate, zinc sulfate, copper sulfate, ammonium sulfate, magnesium sulfate, calcium acetate or potassium dihydrogen phosphate.

The pesticide composition provided by the invention is a preparation known in the field, and soluble powder and water aqua are preferred.

The pesticide composition for relieving herbicide phytotoxicity is suitable for various herbicide phytotoxicity of previous crops, such as residual phytotoxicity of drift herbicide, direct injury of herbicide and the like, and is also suitable for exogenous injury of hormone phytotoxicity, low-temperature freezing injury, salt and alkali injury and the like, and crop diseases such as crop virus diseases, blight and the like.

The pesticide composition for relieving herbicide hazard provided by the invention has the following advantages:

1. the pesticide composition for relieving herbicide hazard provided by the invention comprises the following components:

the compound nitrophenolate comprises compound sodium nitrophenolate, compound potassium nitrophenolate or compound ammonium nitrophenolate and consists of 5-nitroguaiacol salt, o-nitrophenolate and p-nitrophenolate in a weight ratio of 1: 2: 3, the sodium salt, the potassium salt and the ammonium salt are similar bioactive substances. The compound nitrophenolate belongs to a growth-promoting plant growth regulator, is a cell activating agent, is easy to dissolve in water, stable at normal temperature, low in toxicity, free of residue and wide in application range. The compound nitrophenolate can promote the protoplasm flow of cells, increase the cell vitality, promote the growth and development and improve the disease resistance and stress resistance of crops.

Diethyl aminoethyl Hexanoate (DA-6), a chemical name, is diethylaminoethanol caproate, belongs to a growth-promoting plant growth regulator, is easy to dissolve in water, stable at normal temperature and under neutral and acidic conditions, low in toxicity, free of residues and wide in application range. The diethyl aminoethyl hexanoate can improve the activity of plant peroxidase and nitrate reductase, increase the content of chlorophyll, accelerate the photosynthesis speed, enhance the resistance to stress and damage, promote the division and the extension of plant cells, promote the development of root systems and regulate the nutrient balance in vivo.

Chitosan oligosaccharides (Oligosaccharins), also known as amino-oligosaccharides, have a chemical name of beta-1, 4-oligo-glucosamine, belong to polysaccharide bioactive substances, are plant resistance inducers, are easily soluble in water, stable in weak acid and weak alkaline solutions, are slightly toxic, and are easily degradable in the environment. The chitosan oligosaccharide can activate plant cells, promote roots and strong seedlings, promote plant growth, activate a plant immune system, improve the activity of a defense enzyme and an active oxygen scavenging enzyme system related to disease resistance and stress-resistant metabolism, induce the stress resistance of plants, such as disease resistance, cold resistance, high temperature resistance, drought and waterlogging resistance, saline resistance, fertilizer damage resistance and the like, and prevent, alleviate and repair the damage of stress to the plant cells.

The glucans belong to polysaccharide bioactive substances, are novel plant resistance inducers, are slightly toxic and are easily soluble in water. The glucanase as an exogenous regulatory factor can induce plants to generate plant protection matters capable of killing pathogenic microorganisms, and reduce the generation of various crop diseases; can also be used as a growth regulating factor to effectively promote various metabolic activities of plant growth, branching, flowering, fruiting and the like and improve the crop yield.

In the research, the plant growth regulator and the active polysaccharide have synergistic action; the plant growth regulator has compensation effect among the compound nitrophenolate and diethyl aminoethyl hexanoate, active polysaccharide chitosan oligosaccharide and glucan, and the effect of relieving herbicide phytotoxicity. The plant growth regulator compound nitrophenolate and/or diethyl aminoethyl hexanoate is mixed with active polysaccharide chitosan oligosaccharide and/or dextran polyenyl glycosyl within a certain weight proportion range, so that the effect of relieving the phytotoxicity of the herbicide is obviously better than that of the plant growth regulator compound nitrophenolate and/or diethyl aminoethyl hexanoate and that of the active polysaccharide chitosan oligosaccharide and/or dextran polyenyl ethanolamine.

2. Related researches show that the essence of herbicide phytotoxicity is that the invasion amount of exogenous toxic substances exceeds the self resistance and metabolic capability of crops, so that enzyme system disorder and hormone system imbalance in plants are caused.

The pesticide composition for relieving herbicide phytotoxicity provided by the invention utilizes the synergistic effect generated after the growth-promoting plant growth regulator and the active polysaccharide are combined, regulates the active oxygen metabolism in crops, induces the basic resistance, promotes the decomposition of toxic metabolites and toxic exogenous substances, reconstructs an endogenous hormone system with a coordinated proportion, enhances the stress resistance of the crops, resists the stress of a hazard source, slows down the allergic reaction of the crops, relieves the abnormal growth and relieves the phytotoxicity of the herbicide.

3. The pesticide composition for relieving the phytotoxicity of the herbicide can be widely applied to the phytotoxicity of the herbicide of crops such as rice, cotton, peanuts, soybeans, hot peppers, tomatoes and the like, has extremely obvious effect on the phytotoxicity of the herbicide such as 2.4-D butyl ester, tribenuron-methyl and the like which is difficult to relieve symptoms and seriously harmful, and has obvious effect; meanwhile, the herbicide is also suitable for other herbicide hazards, such as isoproturon, atrazine, acetochlor and the like, and can promote crops to quickly recover normal growth after being applied.

In addition, the pesticide composition for relieving the herbicide phytotoxicity has a remarkable effect of relieving the herbicide phytotoxicity, is also suitable for exogenous injuries such as abnormal yellowing, short runt seedling, flower and bud falling and the like caused by hormone phytotoxicity, low-temperature freezing injury, salt and alkali injury and other reasons, and is also suitable for crop diseases such as crop virus diseases, blight and the like.

4. Compared with the prior art popularized at present, the pesticide composition for relieving the phytotoxicity of the herbicide has the outstanding characteristics that the effect of relieving the phytotoxicity of the herbicide is remarkable, the effect of relieving the phytotoxicity of the herbicide is 71.6-93.3 percent, the yield increase effect is 8.7-53.9 percent, the pesticide composition is generally sprayed on the leaf surfaces, the spraying concentration is generally 15-25ppm, and the liquid amount of the pesticide is 450 kg/hm²The dosage of the effective components is 6.75-15.0g/hm². The invention can be sprayed in advance or applied with the herbicide at the same time to prevent the occurrence of herbicide phytotoxicity and can also be used after the occurrence of herbicide phytotoxicity. The amount can be adjusted as desired by those skilled in the art.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In the following examples, the particle size range of all the raw materials is not limited, but in order to satisfy the requirements of fluidity and uniformity of the prepared formulation, the raw material components having a particle size of 0.01 to 0.99mm are preferable. But should not be construed as limiting the invention.

The active ingredients used in the present invention are commercially available raw materials having a high purity, and the purity is less than 100%, but the total of the filler and the active ingredient is not 100% in each example because the purity does not affect the agricultural chemical composition.

Example 1: pesticide composition for relieving herbicide hazard, 1% of compound sodium nitrophenolate, 1% of diethyl aminoethyl hexanoate, 1% of chitosan oligosaccharide and 1% of soluble glucan powder "

1. The components (weight percentage 100%): 1.0% of compound sodium nitrophenolate; 1.0% of diethyl aminoethyl hexanoate; 1.0% of chitosan oligosaccharide; 1.0% of dextrane sugar; calcium chloride as filler.

2. The preparation method comprises the following steps: respectively weighing 1.0g of 98% compound sodium nitrophenolate, 1.0g of 98% diethyl aminoethyl hexanoate, 1.1g of 90% chitosan oligosaccharide, 1.1g of 95% dextran polysaccharide and 95.8g of filler calcium chloride, and uniformly mixing the components.

Example 2: pesticide composition for relieving herbicide hazard, 0.2% of potassium nitrophenolate, 0.2% of diethyl aminoethyl hexanoate, 10% of chitosan oligosaccharide and 10% of soluble powder of glucose "

1. The components (100%): 0.2 percent of compound potassium nitrophenolate; 0.2% of diethyl aminoethyl hexanoate; 10.0% of chitosan oligosaccharide; 10.0% of glucal; and (4) filling potassium chloride.

2. The preparation method comprises the following steps: respectively weighing 0.2g of 98% compound potassium nitrophenolate, 0.2g of 98% diethyl aminoethyl hexanoate, 11.1g of 90% chitosan oligosaccharide, 10.5g of 95% dextran polysaccharide and 78.0g of filler potassium chloride, and uniformly mixing the components.

Example 3: pesticide composition for relieving herbicide hazard, namely 10% of compound ammonium nitrophenolate, 10% of diethyl aminoethyl hexanoate, 0.2% of chitosan oligosaccharide and 0.2% of soluble glucan powder "

1. The components (100%): 10.0 percent of compound ammonium nitrophenolate; diethyl aminoethyl hexanoate 10.0%; 0.2 percent of chitosan oligosaccharide; 0.2% of glucal; and (4) filling urea.

2. The preparation method comprises the following steps: respectively weighing 10.2g of 98% of compound ammonium nitrophenolate, 10.2g of 98% of diethyl aminoethyl hexanoate, 0.2g of 90% chitosan oligosaccharide, 0.2g of 95% dextran polysaccharide and 79.2g of filler urea, and uniformly mixing the components.

Example 4: pesticide composition for relieving herbicide hazard, 20% of compound sodium nitrophenolate, 0.5% of chitosan oligosaccharide and 0.5% of soluble glucan powder "

1. The components (100%): 20.0 percent of compound sodium nitrophenolate; 0.5 percent of chitosan oligosaccharide; 0.5% of glucal; the filler is manganese chloride.

2. The preparation method comprises the following steps: respectively weighing 20.4g of 98% compound sodium nitrophenolate, 0.6g of 90% chitosan oligosaccharide, 0.5g of 95% dextran polysaccharide and 78.5g of filler manganese chloride, and uniformly mixing the components.

Example 5: pesticide composition for relieving herbicide hazard, namely 10% diethyl aminoethyl hexanoate, 0.2% chitosan oligosaccharide and 0.2% glucose soluble powder "

1. The components (100%): diethyl aminoethyl hexanoate 10.0%; 0.2 percent of chitosan oligosaccharide; 0.2% of glucal; and (4) filling zinc sulfate.

2. The preparation method comprises the following steps: respectively weighing 10.2g of 98% diethyl aminoethyl hexanoate, 0.2g of 90% chitosan oligosaccharide, 0.2g of 95% dextran sugar and 89.4g of filler zinc sulfate, and uniformly mixing the components.

Example 6: pesticide composition for relieving herbicide hazard, 1% compound sodium nitrophenolate, 1% diethyl aminoethyl hexanoate and 20% chitosan oligosaccharide soluble powder "

1. The components (100%): 1.0% of compound sodium nitrophenolate; 1.0% of diethyl aminoethyl hexanoate; 20.0% of chitosan oligosaccharide; calcium acetate was used as a filler.

2. The preparation method comprises the following steps: respectively weighing 1.0g of 98% compound sodium nitrophenolate, 1.0g of 98% diethyl aminoethyl hexanoate, 22.2g of 90% chitosan oligosaccharide and 75.8g of filler calcium acetate, and uniformly mixing the components.

Example 7: pesticide composition for relieving herbicide hazard, 1% of compound sodium nitrophenolate, 1% of diethyl aminoethyl hexanoate and 20% of soluble glucan powder "

1. The components (100%): 1.0% of compound sodium nitrophenolate; 1.0% of diethyl aminoethyl hexanoate; 20.0% of dextrane sugar; and a filler of magnesium chloride.

2. The preparation method comprises the following steps: respectively weighing 1.0g of 98% compound sodium nitrophenolate, 1.0g of 98% diethyl aminoethyl hexanoate, 21.1g of 95% dextran polysaccharide and 76.9g of filler magnesium chloride, and uniformly mixing the components.

Example 8: pesticide composition '40% compound sodium nitrophenolate +40% chitosan oligosaccharide soluble powder' for relieving herbicide hazard "

1. The components (100%): 40.0 percent of compound sodium nitrophenolate; 40.0% of chitosan oligosaccharide; and filling material ammonium chloride.

2. The preparation method comprises the following steps: 40.8g of 98% compound sodium nitrophenolate, 44.4g of 90% chitosan oligosaccharide and 14.8g of filler ammonium chloride are weighed respectively and mixed evenly.

Example 9: pesticide composition '5% compound sodium nitrophenolate +20% chitosan oligosaccharide soluble powder' for relieving herbicide hazard "

1. The components (100%): 5.0 percent of compound sodium nitrophenolate; 20.0% of chitosan oligosaccharide; calcium chloride as filler.

2. The preparation method comprises the following steps: respectively weighing 5.1g of 98% compound sodium nitrophenolate, 22.2g of 90% chitosan oligosaccharide and 72.7g of filler calcium chloride, and uniformly mixing the components.

Example 10: pesticide composition for relieving herbicide hazard, namely 40% compound sodium nitrophenolate and 40% soluble glucan powder "

1. The components (100%): 40.0 percent of compound sodium nitrophenolate; 40.0% of dextrane sugar; the filler is potassium dihydrogen phosphate.

2. The preparation method comprises the following steps: 40.8g of 98% compound sodium nitrophenolate, 42.1g of 95% dextran sugar and 17.1g of filler monopotassium phosphate are weighed respectively and evenly mixed to obtain the composition.

Example 11: pesticide composition for relieving herbicide hazard, 20% compound sodium nitrophenolate and 5% soluble powder of glucose "

1. The components (100%): 20.0 percent of compound sodium nitrophenolate; 5.0% of glucal; copper sulfate as filler.

2. The preparation method comprises the following steps: respectively weighing 20.4g of 98% compound sodium nitrophenolate, 5.3g of 95% dextran sugar and 74.3g of filler copper sulfate, and uniformly mixing the components.

Example 12: pesticide composition '40% diethyl aminoethyl hexanoate and 40% chitosan oligosaccharide soluble powder' for relieving herbicide hazard "

1. The components (100%): diethyl aminoethyl hexanoate 40.0%; 40.0% of chitosan oligosaccharide; ferrous sulfate is used as a filler.

2. The preparation method comprises the following steps: 40.8g of 98% diethyl aminoethyl hexanoate, 44.4g of 90% chitosan oligosaccharide and 14.8g of filler ferrous sulfate are weighed respectively and mixed evenly.

Example 13: pesticide composition '20% diethyl aminoethyl hexanoate and 5% chitosan oligosaccharide soluble powder' for relieving herbicide hazard "

1. The components (100%): diethyl aminoethyl hexanoate 20.0%; 5.0% of chitosan oligosaccharide; calcium chloride as filler.

2. The preparation method comprises the following steps: respectively weighing 20.4g of 98% diethyl aminoethyl hexanoate, 5.6g of 90% chitosan oligosaccharide and 74.0g of filler calcium chloride, and uniformly mixing the components.

Example 14: pesticide composition for relieving herbicide hazard, namely 40% diethyl aminoethyl hexanoate and 40% soluble glucan powder "

1. The components (100%): diethyl aminoethyl hexanoate 40.0%; 40.0% of dextrane sugar; and filling ammonium sulfate.

2. The preparation method comprises the following steps: 40.8g of 98% diethyl aminoethyl hexanoate, 42.1g of 95% dextran sugar and 17.1g of filler ammonium sulfate are weighed respectively and mixed evenly.

Example 15: pesticide composition for relieving herbicide hazard, namely 5% diethyl aminoethyl hexanoate and 20% soluble glucan powder "

1. The components (100%): 5.0% of diethyl aminoethyl hexanoate; 20.0% of dextrane sugar; calcium chloride as filler.

2. The preparation method comprises the following steps: respectively weighing 5.1g of 98% diethyl aminoethyl hexanoate, 21.1g of 95% dextran sugar and 73.8g of filler calcium chloride, and uniformly mixing the components.

Example 16: pesticide composition for relieving herbicide hazard, 1% of compound sodium nitrophenolate, 1% of diethyl aminoethyl hexanoate, 0.2% of chitosan oligosaccharide and 0.2% of dextran syrup agent "

1. The components (100%): 1.0% of compound sodium nitrophenolate; 1.0% of diethyl aminoethyl hexanoate; 0.2 percent of chitosan oligosaccharide; 0.2 percent of glucal.

2. The preparation method comprises the following steps: weighing 1.0g of 98% compound sodium nitrophenolate, 1.0g of 98% diethyl aminoethyl hexanoate, 0.2g of 90% chitosan oligosaccharide and 0.2g of 95% dextran sugar, putting into a 100ml volumetric flask, adding water to dissolve, and fixing the volume to 100ml to obtain the aqua.

Example 17: pesticide composition '3% compound sodium nitrophenolate +1% chitosan oligosaccharide aqueous solution' for relieving herbicide hazard "

1. The components (100%): 3.0 percent of compound sodium nitrophenolate; 1.0 percent of chitosan oligosaccharide.

2. The preparation method comprises the following steps: weighing 3.1g of 98% compound sodium nitrophenolate and 1.1g of 90% chitosan oligosaccharide, putting into a 100ml volumetric flask, adding water to dissolve, and fixing the volume to 100ml to obtain the aqueous solution.

Experimental example 1

1. Test treatments and materials:

the test treatments and spray concentrations are shown in table 1. Among them, treatments 1 to 17 are the herbicide phytotoxicity-mitigating pesticide compositions provided in examples 1 to 17 of the present invention, respectively.

Treatments 18-19 were respectively:

and (4) treatment: control ck₁(compounding of plant growth regulator): 10% of compound sodium nitrophenolate and 10% of diethyl aminoethyl hexanoate soluble powder;

and (19) treatment: control ck₂(compounding of active polysaccharide): 10% chitooligosaccharide +10% dextran soluble powder.

Treatments 20-23 were:

and (3) treatment 20: control ck₃: 1.8% sodium nitrophenolate aqueous solution (commercially available);

and (3) processing: control ck₄: 5% diethyl aminoethyl hexanoate aqueous solution (commercially available);

and (4) processing 22: control ck₅: 2% amino-oligosaccharin aqua (commercially available);

and (3) treatment: control ck₆: 0.5% aqueous solution of dextrane (commercially available).

Treatment 24 is clear water control ck₀。

The raw materials for preparing and treating 18-19 are as follows: 98 percent of compound sodium nitrophenolate, 98 percent of diethyl aminoethyl hexanoate, 90 percent of chitosan oligosaccharide, 95 percent of glucan and calcium chloride as a filler. Selecting particles with the raw material granularity of 0.01-0.99mm, weighing and mixing according to the following proportion:

process 18 (ck)₁): 10.2g of 98% compound sodium nitrophenolate, 10.2g of 98% diethyl aminoethyl hexanoate and 79.6g of calcium chloride.

Process 19 (ck)₂): 11.1g of 90% chitosan oligosaccharide, 10.5g of 95% dextran polysaccharide and 78.4g of calcium chloride.

Table 1: test treatment set-up

2. Test method

In the experiment, potted Taihe cowpea No. 2 cowpea is used as a test crop, after the 1 st alternate true leaf is unfolded, 2400 times of liquid (300 ppm, 1/4 lethal concentration) of 72 percent 2.4-D butyl ester herbicide is sprayed on the leaf surface, and serious herbicide phytotoxicity is artificially created. 4 holes are reserved in each pot, and two seedlings are reserved in each hole; two pots were in 1 group, repeated 4 times. The first spraying is started on the 2 nd day of artificial herbicide hazard manufacturing, a medical sprayer is used for uniformly spraying, the spraying concentration of each treatment is adjusted to about 20ppm according to the effective components, and the dilution times are shown in table 1; spraying once every 7 days, and spraying 2 times in total. And 7 days after the second spraying, investigating the phytotoxicity condition and calculating the detoxification effect. The phytotoxicity grading standards are as follows:

level 0: the growth is normal;

level 1: mild malformation of heart leaves, mild dwarfing of plants;

and 3, level: mild malformation of heart leaves and dwarfing of plants;

and 5, stage: heart leaves are seriously deformed, and plants are obviously dwarfed;

and 7, stage: the whole plant died.

The detoxifying effect was calculated as follows (1) and (2).

（1）

3. And (3) test results: the test results are shown in Table 2.

Table 2: summary table of toxicity test results of pesticide composition for relieving phytotoxicity of herbicide

Table 2 the results show:

1) the treatment 1-17 is a mixture of a plant growth regulator and active polysaccharide, wherein the weight ratio of the plant growth regulator to the active polysaccharide is 1: 0.02-1: 50, control ck₁、ck₃、ck₄Respectively as mixed and single agent of plant growth regulator and control ck₂、ck₅、ck₆Respectively are a mixed agent and a single agent of active polysaccharide, the detoxification effect of treatment 1-17 is 74.3% -90.4%, and both are remarkably superior to the detoxification effect of each control by 47.1% -53.8%, which shows that the plant growth regulator and the active polysaccharide have synergistic effect in the pesticide composition for relieving the phytotoxicity of the herbicide.

2) In the treatments 2-7, the component ratio of the plant growth regulator to the active polysaccharide is 1:50, 1:0.02, 1: 0.05, 1: 0.04, 1: 10. 1: 10, the component ratio is deviated from 1:1 greatly, and the detoxification effect is 74.3 to 79.1 percent; in the treatment 1 and the treatment 8-17, the component ratio of the plant growth regulator to the active polysaccharide is 1: 0.2-1: 4, the component ratio is close to 1:1, and the detoxification effect is 80.4-90.4%; treatments with component ratios close to 1:1 were superior to treatments with component ratios deviating more from 1: 1.

3) In the treatments 1, 8, 10, 12 and 14, the component ratio of the plant growth regulator to the active polysaccharide is 1:1, the effective components are four components of plant growth regulator compound sodium nitrophenolate, diethyl aminoethyl hexanoate, active polysaccharide chitosan oligosaccharide and glucan respectively, or two components of mixing any one of the plant growth regulator compound sodium nitrophenolate and diethyl aminoethyl hexanoate with any one of the active polysaccharide chitosan oligosaccharide and glucan respectively, the detoxification effects are 90.4%, 89.4%, 89.9%, 89.2% and 89.9%, no significant difference exists among the treatments, and the compensation effect exists among the plant growth regulator compound sodium nitrophenolate and diethyl aminoethyl hexanoate, the active polysaccharide chitosan oligosaccharide and glucan in the compounded herbicide phytotoxicity relieving pesticide composition.

4. Conclusion of the experiment

The herbicide phytotoxicity relieving pesticide composition is compounded with one or two of plant growth regulator compound nitrophenolate and diethyl aminoethyl hexanoate and one or two of active polysaccharide chitosan oligosaccharide and dextran, and has obvious herbicide phytotoxicity relieving effect superior to that of plant growth regulator compound nitrophenolate, diethyl aminoethyl hexanoate and active polysaccharide chitosan oligosaccharide and dextran. In the range of experimental setting, the plant growth regulator and the active polysaccharide have synergistic action in the pesticide composition for relieving the phytotoxicity of the herbicide; the plant growth regulator has compensation effect among the compound nitrophenolate and diethyl aminoethyl hexanoate, and among the active polysaccharide chitosan oligosaccharide and the glucose.

Experimental example 2:

1. test materials and methods

The test land is arranged in Korean villages in Qingfeng county, the fertilizer and water conditions are good, the irrigation and drainage are convenient, the soil fertility is uniform, and the sandy soil is good. Spraying tribenuron-methyl on wheat in the first crop to cause flowers in the later cropProducing raw herbicide phytotoxicity. The peanut variety Puhua No. 5 is interplanted for 15 days in 5 months, wide and narrow rows are planted, the narrow rows are 0.25 meter, the wide rows are 0.55 meter, the hole spacing is 0.167 meter, 2 grains are planted in each hole, and the density is 1 ten thousand holes/mu. Cell area 30m²10 rows of regions, 7.5m long. Other field management in the test process is normal.

Test agents: example 17 (3% sodium nitrophenolate +1% chitosan oligosaccharide aqueous solution).

Control agents: 5% diethyl aminoethyl hexanoate water solution, a commercial product.

The experimental treatment is set as follows: the herbicide phytotoxicity-relieving pesticide composition prepared in example 17 was prepared in 1500-fold liquid (26.7 ppm), 2000-fold liquid (20.0 ppm), 2500-fold liquid (16.0 ppm), 3000-fold liquid (13.3 ppm), 5% diethyl aminoethyl hexanoate aqueous solution (1500-fold liquid (33.3 ppm) and clear water control 6 treatments, 4 replicates, 24 cells in total, and arranged in random blocks.

The first application is started on 21 days 6 months at the early stage of phytotoxicity development, a worker-farmer 16 type manual sprayer with the spray hole diameter of 0.7mm is used for uniformly spraying according to the test treatment, the liquid medicine is sprayed to a cell by 1.35kg, the liquid medicine is sprayed to each mu by 30kg, and the spraying is carried out once every 5 days for 2 times.

Each cell is selected 5 points diagonally, and each point is marked with 5 holes. The base number of phytotoxicity was investigated before the application of the drug, and the phytotoxicity of the marker strain was investigated 7 days after the second application.

The phytotoxicity grading standards are as follows:

level 0: the plant grows normally or the phytotoxicity plant grows normal new leaves;

level 1: the plants are slightly dwarfed, and new leaves become small (no new roots);

and 3, level: the plants were severely stunted, new leaves yellow (root system blackened);

and 5, stage: the plant is seriously dwarfed, and new leaves die;

and 7, stage: the whole plant died.

The detoxifying effect was calculated according to the above formula (1) and the following formula (3).

2. And (3) test results: the effect of mitigating herbicide injury is shown in table 3.

Table 3: pesticide composition for relieving herbicide phytotoxicity of peanut

Table 3 the results show: the detoxification effects of the 1500-fold solution (26.7 ppm), the 2000-fold solution (20.0 ppm), the 2500-fold solution (16.0 ppm) and the 3000-fold solution (13.3 ppm) of the pesticide composition for alleviating herbicide phytotoxicity prepared in example 17 were 83.6%, 82.2%, 77.6% and 68.5%, respectively, and the detoxification effect of the 1500-fold solution (33.3 ppm) of the control agent 5% diethyl aminoethyl hexanoate aqueous solution was 46.1%. Through analysis of variance, the detoxification effects of 1500-fold liquid and 2000-fold liquid of the pesticide composition for relieving phytotoxicity of the herbicide prepared in example 17 have no significant difference, and are both significantly better than 2500-fold liquid and 3000-fold liquid; the detoxification effect of each treatment concentration of the pesticide composition for relieving herbicide phytotoxicity prepared in example 17 is significantly better than that of 1500 times of that of a 5% diethyl aminoethyl hexanoate aqueous solution as a medicament control.

The test result shows that: in the pesticide composition for relieving herbicide hazard provided by the invention, the embodiment 17 has a remarkable effect of relieving the herbicide hazard of the peanut tribenuron-methyl previous crop, and the optimal concentration is 1500-fold 2000-fold liquid (20.0-26.7 ppm) and 2500-fold 3000-fold liquid (13.3-16.0 ppm) also have production significance.

Experimental example 3:

1. test materials and methods

The test field is set in Puyang county pear garden, the conditions of fertilizer and water are good, the irrigation and drainage are convenient, and the soil fertility is uniform. The spraying of tribenuron-methyl on wheat in the first crop causes the yield of rice in the next cropThe herbicide damage is caused by the raw herbicide, the rice variety Zhengdao No. 18, the rice transplanting is carried out in 13 days in 6 months, the planting is carried out at equal row spacing, the row spacing is 0.3 meter, the hole spacing is 0.1 meter, and the density is 2.2 ten thousand clumps/mu. Cell area 27m²15 rows of fields, 6m long. Other field management in the test process is normal.

Test agents: example 12 (40% diethyl aminoethyl hexanoate +40% chitosan oligosaccharide soluble powder).

Control agents: 8% diethyl aminoethyl hexanoate soluble powder, which is commercially available.

And (3) test treatment: the pesticide composition for relieving herbicide phytotoxicity prepared in example 12 is prepared by 26.7ppm (30000 times liquid), 20ppm (40000 times liquid), 16ppm (50000 times liquid), 53.3ppm (1500 times liquid) of 8% diethyl aminoethyl hexanoate soluble powder serving as a control agent and 5 treatments of a clean water control. The experiment was repeated 4 times for 20 cells in total and arranged in random blocks.

The first application is started at 26 days in 6 months at the early stage of phytotoxicity development, a worker-farmer 16 type manual sprayer with the spray hole diameter of 0.7mm is used for uniformly spraying according to the test treatment, the liquid medicine is sprayed to a cell by 1.2kg, the liquid medicine is sprayed to each mu by 30kg, and the spraying is carried out once every 5 days for 2 times.

Each cell is diagonally selected at 5 points, each marked with 5 clusters. The base number of phytotoxicity was investigated before the application, and the phytotoxicity of the shoot in the marker cluster was investigated 7 days after the second application.

The phytotoxicity grading standards are as follows:

level 0: the stem shoots grow normally or the phytotoxicity stem shoots grow normal new leaves;

level 1: a small amount of chlorosis macula appears at the base of heart lobes, and the heart lobes do not curl;

and 3, level: the young leaves are yellow and slightly curled;

and 5, stage: yellowing, curling and malformation of new leaves;

and 7, stage: the plants were stunted, green lost or dead.

The detoxifying effect was calculated according to formula (1) shown in experimental example 1 and formula (3) shown in experimental example 2.

2. And (3) test results: the effect of alleviating herbicide injury of peanut stubble is shown in table 4.

Table 4: pesticide composition for relieving herbicide phytotoxicity of paddy field and eliminating herbicide phytotoxicity of paddy field

Table 4 the results show: the pesticidal composition for alleviating herbicide phytotoxicity prepared in example 12 had detoxification effects of 82.6%, 81.3% and 77.0% at 26.7ppm (30000-fold liquid), 20.0ppm (40000-fold liquid) and 16.0ppm (50000-fold liquid), respectively, and 53.7% at 53.3ppm (1500-fold liquid) as a control agent 8% diethyl aminoethyl hexanoate soluble powder. Through analysis of variance, the pesticide composition for relieving herbicide phytotoxicity prepared in example 12 has no significant difference between the detoxification effects of 26.7ppm and 20.0ppm, and both are significantly better than 16.0 ppm; the detoxification effect of each treatment of the pesticide composition for relieving the phytotoxicity of the herbicide prepared in example 12 is remarkably superior to 53.3ppm of the pesticide control 8% diethyl aminoethyl hexanoate soluble powder.

Therefore, the pesticide composition for relieving the phytotoxicity of the herbicide prepared in the example 12 has a remarkable effect of relieving the phytotoxicity of the tribenuron-methyl previous crop herbicide in the paddy field, and the optimal concentration of the pesticide composition is 20.0-26.7ppm, and 16.0ppm also has production significance.

Experimental example 4:

1. test materials and methods

The test field is set in Puyang county Huxiang, has two soil qualities, is convenient for drainage and irrigation, and has a 3:1 wheat cotton sleeve, a cotton institute number 29 in cotton variety, a row spacing of 1 meter and the like, and the density of 2500 plants per mu. Spraying 2.4-D butyl ester herbicide adjacently, and generating phytotoxicity along with wind drift.

Test agents: example 8 (40% sodium nitrophenolate +40% chitosan oligosaccharide soluble powder).

Control agents: 1.8% of compound sodium nitrophenolate aqueous agent, and is a commercial product.

And (3) test treatment: the pesticide composition for alleviating herbicide damage prepared in example 8 was treated with 26.7ppm (30000-fold liquid), 20ppm (40000-fold liquid), 16ppm (50000-fold liquid), 1.8% of a control agent, 18ppm (1000-fold liquid) of a compound sodium nitrophenolate aqueous solution and 5 clear water controls.

The test is repeated for 4 times, the random block arrangement is carried out, 4 rows of blocks are arranged, the row length is 7.5 meters, and the area of a cell is 30m². The field spraying adopts a farmer 16-type sprayer, the aperture of a spraying sheet is 0.7mm, the amount of the pesticide liquid sprayed in a community is 1.4kg, and the amount is about 30kg per mu.

The cotton field shows 2.4-D butyl ester herbicide phytotoxicity symptom in 21 days in 6 months, the first pesticide application is started on the 3 rd day, the first pesticide application is started once every 5 days, the second pesticide application is started on 28 days in 6 months, and the pesticide application is carried out for 2 times in total. And (3) surveying and recording the phytotoxicity conditions of the two middle lines of the community before pesticide application and 7 days after the second pesticide application, and observing the influence on the occurrence condition of cotton blight and verticillium wilt.

The grading standard of phytotoxicity investigation is as follows:

level 0: no phytotoxicity symptom, or the phytotoxicity plants grow normal new leaves;

level 1: the newly born leaves are slightly malformed, and the plants grow normally;

and 3, level: the newly born leaves are slightly malformed, and the plants are slightly dwarfed;

and 5, stage: the new leaves are deformed and the plants are dwarfed;

and 7, stage: the newly grown leaves were severely misshapen and the plants stopped growing.

The detoxifying effect was calculated according to formula (1) shown in experimental example 1 and formula (3) shown in experimental example 2.

2. And (4) analyzing results: the test results are shown in Table 5.

Table 5: pesticide composition for relieving herbicide hazard of cotton

Table 5 the results show: the pesticide composition for alleviating phytotoxicity of herbicide prepared in example 8 had detoxification effects of 80.3 ppm (30000-fold liquid), 79.7 ppm (40000-fold liquid), and 73.5 ppm (50000-fold liquid), respectively, and the control agent 1.8% sodium nitrophenolate 18ppm (1000-fold liquid) had detoxification effects of 47.5%. The difference of the detoxification effects of the tested medicaments of 26.7ppm and 20ppm is not obvious, and the detoxification effects of the two treatments are remarkably superior to 16 ppm; the treatment of the test medicament is remarkably superior to that of a control 1.8 percent compound sodium nitrophenolate aqueous solution. The optimal concentration of the 2.4-D butyl ester herbicide phytotoxicity relieving pesticide composition for relieving the drift of the cotton field prepared in the example 8 is 20.0-26.7ppm, and 16.0ppm can also be used in production.

Through experimental observation, after each treatment of spraying the pesticide composition for relieving the phytotoxicity of the herbicide in the test area, no cotton fusarium and verticillium wilt is found, and the disease of the pesticide control area is light; the cotton verticillium wilt is more severe in non-test areas of the same plot. The pesticide composition for relieving the phytotoxicity of the herbicide prepared in the example 8 has good prevention and control effects on cotton fusarium wilt and verticillium wilt.

Experimental example 5:

1. test materials and methods

The test is carried out in four villages in Huaxian county, Henan province, the soil is two-in-one, the terrain is flat, the fertility is uniform, the irrigation and drainage are convenient, and the soil fertility is medium. The tested crop watermelon is sprayed with 2.4-D butyl ester herbicide adjacently to cause drift herbicide phytotoxicity. The watermelon is Zhengkang 5503, is cultivated in 15 days in 5 months and is planted in 10 days in 6 months, the row spacing is 1.8m, the plant spacing is 0.5m, and the density is about 750 plants/mu.

Test agents: example 1 (1% sodium nitrophenolate +1% diethyl aminoethyl hexanoate +1% chitosan oligosaccharide +1% dextran soluble powder).

Control agents: 2% chitosan oligosaccharide aqua, commercially available product.

And (3) test treatment: the pesticide composition for relieving herbicide phytotoxicity prepared in example 1 is treated by 26.7ppm (1500 times liquid), 20ppm (2000 times liquid), 16ppm (2500 times liquid), 13.3ppm (3000 times liquid), 2% chitosan oligosaccharide aqueous solution as a control agent, 20ppm (1000 times liquid) and 6 clear water controls.

The test is repeated for 4 times, and the test is arranged in random blocks, wherein the cell is 4 rows of cells, the length of the row is 6 meters, and the area of the cell is 43.2 square meters. The large field spray adopts a Mount Taishan-18 type motor-driven atomizing sprayer, and the amount of the pesticide liquid sprayed in a community is 2kg, which is about 30kg per mu.

The watermelon shows 2.4-D butyl ester herbicide phytotoxicity symptom in 25 days in 6 months, the first pesticide application is started in 3 days, the first pesticide application is started in 5 days, the second pesticide application is started in 2 days in 7 months, and the total pesticide application is carried out for 2 times. And (3) investigating and recording the phytotoxicity condition of the middle two lines of the community before the first pesticide application and 7 days after the second pesticide application, and observing the influence on the occurrence condition of watermelon virus diseases.

The grading standard of phytotoxicity investigation is as follows:

level 0: the whole plant is disease-free or the stem tip grows normal heart leaves;

level 1: mild malformation of neolobes;

and 3, level: malformation of new leaves, shrinkage distortion, shortening of internodes;

and 5, stage: the leaves are chicken claw-shaped, the stem becomes thin, and the head of the stem is shriveled;

and 7, stage: the leaves were severely chicken claw shaped and growth stopped.

The detoxifying effect was calculated according to formula (1) shown in experimental example 1 and formula (3) shown in experimental example 2.

2. And (4) analyzing results: the test results are shown in Table 6.

Table 6: pesticide composition for relieving herbicide hazard and relieving 24-D butyl ester hazard of watermelons

Table 6 the results show: 26.7ppm (1500-time liquid), 20ppm (2000-time liquid), 16ppm (2500-time liquid) and 13.3ppm (3000-time liquid) of the pesticide composition for relieving herbicide phytotoxicity of the herbicide prepared in example 1 respectively have the effects of relieving 2.4-D butyl ester herbicide phytotoxicity of watermelons of 84.5%, 82.8%, 80.4% and 76.6% and the detoxification effect of a control medicament of 20ppm (1000-time liquid) of 2% chitosan oligosaccharide aqueous solution is 60.5%. The differences of the detoxification effects of the tested medicaments of 26.7ppm, 20ppm and 16ppm are not obvious, and the detoxification effects of the three treatments are remarkably superior to that of 13.3 ppm; the treatment of the test medicament is remarkably superior to the control 2% chitosan oligosaccharide aqueous solution. Experiments show that the pesticide composition for relieving the phytotoxicity of the herbicide prepared in the example 1 has a remarkable effect of relieving the phytotoxicity of the 2.4-D butyl ester herbicide with drift of watermelon, and the optimal concentration is 16.0-26.7 ppm.

Through experimental observation, no watermelon virus disease is found in each treatment of spraying the pesticide composition for relieving herbicide hazard in a test area, and the disease is lighter in a control area; the watermelon virus disease is serious in non-test areas of the same plot. The pesticide composition for relieving herbicide hazard prepared in the example 1 has a certain prevention and treatment effect on watermelon virus diseases.

Experimental example 6:

1. test agents: example 1 (1% sodium nitrophenolate +1% diethyl aminoethyl hexanoate +1% chitosan oligosaccharide +1% dextran soluble powder).

The test is carried out in the permanently economic saline-alkali soil in the transport city, the terrain is flat, the fertility is uniform, the pH value is 9.0, and the perennial crops are stressed by saline and alkaline after seedling emergence and grow slower than the non-saline-alkaline soil.

The test crop cotton, No. 49 cotton institute of variety, is planted in wide and narrow rows, the wide rows are 1.0 meter, and the narrow rows are 0.5 meter. Sowing the seedlings in 25 days in 4 months, aligning the seedlings in 8 days in 5 months, and setting the seedlings to 6000 plants/mu in 18 days in 5 months.

The test adopts a pairing design, two treatments are set, and the process is repeated for 4 times by taking pairs as units. The cell is a 6-row cell with the length of 6m and the area of 27m². The amount of the pesticide sprayed in the residential area is 1.2kg, and is about 30kg per mu.

The experimental treatment is set as follows: example 1 (1% sodium nitrophenolate +1% diethyl aminoethyl hexanoate +1% chitosan oligosaccharide +1% soluble powder dextran) 2000 fold solution (20 ppm), clear water control.

The cotton seedlings grow slowly after fixed seedling, and the spraying of the example 1 is started after 26 days in 5 months, and the spraying is carried out once every 5 days for 2 times. And (5) surveying and recording the plant heights of two rows of cotton seedlings in the middle of each cell after spraying for 7 days for the second time, and carrying out t-test.

2. And (3) test results: the test results are shown in Table 7.

Table 7: statistical table for plant height of cotton seedlings in each treatment district

t_{Measured in fact}=8.76**t_0.05=3.18 t_0.01=5.84

Table 7 the results show: after the pesticide composition for relieving herbicide phytotoxicity is sprayed on cotton seedlings in example 1, the stress of salt and alkali hazards on the cotton seedlings is relieved, the growth of the cotton seedlings is promoted, the plant height is averagely increased by 19.5%, and the difference reaches a very significant level.

Experimental example 7:

1. test materials and methods

The effective components are as follows: 1% of compound sodium nitrophenolate, 1% of diethyl aminoethyl hexanoate, 1% of chitosan oligosaccharide and 1% of dextranase

And (3) test treatment: respectively taking calcium chloride, potassium chloride, ammonium chloride, magnesium sulfate, potassium acetate, monopotassium phosphate and urea as fillers, and respectively treating the prepared pesticide composition containing the effective components by 1-7 times; the water aqua of the pesticide composition containing the effective components is treatment 8; clean water pairAccording to ck₀。

The preparation method comprises the following steps: soluble powder: selecting raw material components with the particle size of 0.01-0.99mm, respectively weighing 1.0g of 98% compound sodium nitrophenolate, 1.0g of 98% diethyl aminoethyl hexanoate, 1.1g of 90% chitosan oligosaccharide and 1.1g of 95% dextran sugar, sequentially weighing fillers of calcium chloride, potassium chloride, ammonium chloride, magnesium sulfate, potassium acetate, potassium dihydrogen phosphate and 95.8g of urea, and respectively and uniformly mixing with the effective component components. (II) water agent: weighing 1.0g of 98% compound sodium nitrophenolate, 1.0g of 98% diethyl aminoethyl hexanoate, 1.1g of 90% chitosan oligosaccharide and 1.1g of 95% dextran sugar, putting into a 100ml volumetric flask, adding water to dissolve and fixing the volume to 100 ml.

The test method was the same as in example 1.

2. And (3) test results: see Table 8

Table 8: effect of different fillers on detoxification Effect

Table 8 results achieved: the pesticide composition for relieving the phytotoxicity of the herbicide is prepared by taking soluble inorganic salts or organic matters containing plant nutrients such as calcium chloride, potassium chloride, ammonium chloride, magnesium sulfate, potassium acetate, monopotassium phosphate, urea and the like as fillers, wherein the pesticide composition comprises 1% of compound sodium nitrophenolate, 1% of diethyl aminoethyl hexanoate, 1% of chitosan oligosaccharide and 1% of soluble glucan powder, and the phytotoxicity of 72% of 2.4-D butyl ester herbicide is released by 87.0-88.6%; the detoxification effect of the compound sodium nitrophenolate 1%, the diethyl aminoethyl hexanoate 1%, the chitosan oligosaccharide 1% and the glucan water solution 1% is 88.8%. Through difference significance analysis, the difference between each treatment of different filler soluble powder and the water aqua is not significant.

The results show that: different fillers and different formulations in the pesticide composition provided by the invention have no obvious influence on the detoxification effect.

Experimental example 8

The herbicide harm-alleviating pesticide composition prepared in example 8 was subjected to a large-area multipoint experiment demonstration on the herbicide harm of crops such as rice, cotton, peanut, soybean, pepper, tomato and the like, and the treatment was set to 20ppm (40000 times liquid) of the herbicide harm-alleviating pesticide composition prepared in example 8 and 2 treatments with clear water as a control.

Exemplary results of the tests are shown in Table 9.

Table 9: example 8 summary of exemplary results of herbicide hazard mitigation tests

Table 9 the results show that: the pesticide composition for relieving herbicide phytotoxicity prepared in the embodiment 8 has obvious effects of detoxifying and increasing yield, 71.6% -93.3% of herbicide phytotoxicity relieving effect and 8.7% -53.9% of yield increasing effect.

Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.