CN103211581B - Temperature-and-illumination-controllable type retinal photo-injury quantitative research device - Google Patents

The invention relates to a retinal photo-injury quantitative research device, in particular to a temperature-and-illumination-controllable type retinal photo-injury quantitative research device. The research device comprises a camera obscura, a light-emitting diode (LED) face light source, a light source driving unit, a semiconductor refrigeration sheet, a refrigeration sheet driving unit, a photo-sensor, a temperature sensor and a control unit. The light source driving unit drives the LED face light source, the refrigeration sheet driving unit drives the semiconductor refrigeration sheet, the LED face light source is arranged in the camera obscura, and the semiconductor refrigeration sheet, the photo-sensor and the temperature sensor are arranged in a test area. By means of the face light source, the semiconductor refrigeration sheet, the photo-sensor, the temperature sensor and a control unit with a single chip mirco-processor serving as a core, the research device can adjust and control a light strength and temperature device in a sealed space, achieves double control and quantitative monitoring of light strength and temperature, and is fast in reaction, stable in result, high in precision and particularly suitable for photo-injury test research at an isolated cell level.

Temperature and illumination controllable type retina photodamage quantitative study device

Technical field

The present invention relates to a kind of retina photodamage quantitative study device, specifically a kind of temperature and illumination controllable type retina photodamage quantitative study device.

Background technology

Photic damage, i.e. excessively strong light or look at for a long time the infringement that light source causes retina straight.The instrument being applied to quantitatively or the photic damage of qualitative investigation retina cell is studied is called to photic damage research device.Photic damage research device generally includes a confined space, a luminous body and a light intensity controlling device etc., by regulating the brightness of luminous body, to the cell in confined space or laboratory animal carry out in short-term, strong illumination reaches the object of photic damage.Yet, aspect photic damage experimentation, there is no unified stable experimental facilities both at home and abroad and control temperature and the illumination of experimental situation, and take the simple instrument self carrying to carry out more at present, the efficiency of its experiment, repeatability and credibility are all lower.Meanwhile, along with photic damage is tested by qualitative to quantitative development trend.Traditional point source is because its inherent limitation is difficult to reach the light intensity of homogeneous, and exists and be difficult to realize digitized regulation and control, and the problems such as Real-Time Monitoring, cannot meet the experiment demand of research that light is hindered in some occasion.As Feng M etc. is publishing during relative photo damage that the e38690 page of PLoS One delivers studies at the Int J Ophthalmol 151-157 page of publishing for 2012 and Ueki Y etc. for 2012, the research of photic damage experiment is carried out in employing in the mode of the fluorescent lamp of the one or more certain wavelength of inside, darkroom placement, cannot guarantee that in experiment, cell is accepted the uniformity of illumination, and cannot formulate unified, believable standard to the evaluation of experiment.And in photic damage research device, temperature is as the significant variable of photic damage research, in the research of quite a few Chinese scholars, be left in the basket, it may cause stability and the repeatability of experiment greatly to reduce.

Summary of the invention

The object of the invention is to provide in order to overcome the deficiencies in the prior art a kind of temperature and illumination controllable type retina photodamage quantitative study device, to realize the problem to light intensity stable homogeneous regulation and control in a certain plane, be applicable to the quantitative temperature adjusting requirement of cell injury experiment simultaneously.

For achieving the above object, the invention discloses a kind of temperature and illumination controllable type retina photodamage quantitative study device, it is characterized in that: comprise camera bellows, LED area source, light source driving units, semiconductor chilling plate, cooling piece driver element, optical sensor, temperature sensor and control unit, described light source driving units driving LED area source, cooling piece drive unit drives semiconductor chilling plate, described LED area source is arranged in camera bellows and direct projection Experimental Area, described semiconductor chilling plate, optical sensor and temperature sensor are arranged in Experimental Area, described control unit be take chip microprocessor and is provided with first input end and the second input as core, described optical sensor is connected with the second input with control unit first input end respectively with the output of temperature sensor, described control unit is also provided with the first outfan and is connected with light source driving units with cooling piece driver element respectively with the second outfan, described control unit is provided with real-time luminosity value memorizer, brightness settings memorizer, real time temperature value memorizer, desired temperature memorizer and comparator, described comparator compares the numerical value in real-time luminosity value memorizer and brightness settings memorizer and real time temperature value memorizer and desired temperature memorizer, described control unit is controlled LED area source and semiconductor chilling plate according to comparative result by the first outfan and the second outfan.

Compared with prior art, the control unit that the present invention is core by installation surface light source, conductor cooling piece, optical sensor, temperature sensor and the chip microprocessor of take, can regulate and control light intensity and the temperature device in confined space by digitized, dual control and quantitative monitoring to temperature and light intensity have been realized, reaction is fast, result is stable, precision is high, is particularly useful for the photic damage experimentation in isolated cells level.

Below in conjunction with drawings and Examples, the present invention is further described.

Accompanying drawing explanation

Fig. 1 is the structure perspective view of the specific embodiment of the invention;

Fig. 2 is specific embodiment of the invention electricity theory diagram.

The specific embodiment

As shown in 1 figure, temperature and illumination controllable type retina photodamage quantitative study device comprise camera bellows 1, LED area source 2, light source driving units, semiconductor chilling plate 3, cooling piece driver element, optical sensor 4, temperature sensor 5 and control unit, described light source driving units driving LED area source 2, cooling piece drive unit drives semiconductor chilling plate 3.The shape of camera bellows 1 can be as required, in this specific embodiment, camera bellows 1 is cuboid, described LED area source 2 matches with camera bellows 1 intracavity bottom shape and is installed in bottom, described LED area source 2 formation Experimental Areas, top, described LED area source 2 tops are provided with culture dish shelf 6, described culture dish shelf 6 be arranged in parallel with LED area source 2, described LED area source 2 direct projection Experimental Areas, like this can be so that Experimental Area beam intensity ratio be more even, culture dish shelf 6 adopts high clear sheet to make, in order to detect in time light intensity and temperature, described optical sensor 4 and temperature sensor 5 are arranged on culture dish shelf 6, in order to detect more exactly, a plurality of optical sensors 4 and temperature sensor 5 can be set in this device, this is evenly distributed on Experimental Area, in order conveniently to carry out zoopery, can also in described Experimental Area, be provided with animal holder 7.In this specific embodiment, light intensity sensor is selected as TSL2561, an inner light sensitive diode (passage 0) and an infrared response light sensitive diode (passage 1) and two the integrated integral a/d converters of connecting of this light intensity sensor, photosensitive current conversion can be become to a numeral output, there is the ability that nearly photopic response in 20 dynamic ranges is provided.LM35 is selected in temperature sensor 5, and these temperature sensor 5 output voltages are directly proportional to Celsius temperature, and the linearity is good, highly sensitive, moderate accuracy, and its output sensitivity is 10.0MV/ ℃, and precision reaches 0.5 ℃, and its measuring range is-55---150 ℃.Self-heating effect low (0.08 ℃) in static temperature, running voltage is wider, can be 4---normal work in the power supply voltage range of 20V, and power consumption is economic, and operating current is generally less than 60uA, and output impedance is low, when 1MA load, is 0.1 Ω.Known according to the output characteristics of LM35, when temperature is 0---while converting between 150 ℃, the voltage that its outfan is corresponding is 0---150V, this voltage is delivered to 3 Semi-digital display gauge heads detection signal input of (being comprised of ICL7107 and circuits) after potentiometer W3 dividing potential drop, when the voltage of input input is 150V, by regulator potentiometer, making the numerical value showing is 150.0, and the numerical value that digital display meter head shows after adjusting is exactly the temperature value of actual measurement.Described semiconductor chilling plate 3 is arranged on the sidewall of culture dish shelf 6 tops, and semiconductor chilling plate 3 heats refrigeration system with tradition and compares, have that volume is littlely easy to carry, puts that temperature is controlled, temperature control accurately rapidly and advantages of environment protection.Described control unit is arranged on the below of camera bellows 1 inner chamber LED area source 2, as shown in Figure 2, described control unit be take arm processor STM32F103 as core, be provided with first input end and the second input, described optical sensor 4 is connected with the second input with control unit first input end respectively with the output of temperature sensor 5, described control unit is provided with also the first outfan and is connected with light source driving units with cooling piece driver element respectively with the second outfan, described control unit is provided with real-time luminosity value memorizer, brightness settings memorizer, real time temperature value memorizer, desired temperature memorizer and comparator, described comparator compares the numerical value in real-time luminosity value memorizer and brightness settings memorizer and real time temperature value memorizer and desired temperature memorizer, described control unit is controlled LED area source 2 and semiconductor chilling plate 3 according to comparative result by the first outfan and the second outfan, real-time luminosity value memorizer wherein, brightness settings memorizer, real time temperature value memorizer and desired temperature memorizer have internal storage to serve as, comparator is realized by internal processes.Light source driving units is controlled LED area source 2 and is adopted pulse width modulation modes, and LED connects with inductance, and in parallel with fly-wheel diode, when derailing switch conducting, power supply is powered to LED light source through inductance like this, inductance coil energy storage; When derailing switch ends, power supply breaking circuit, is powered to LED light source by inductance coil, discharges the energy storing, thereby make the electric current through LED light source, in derailing switch cutout, is still can maintain, brightness stability.In order to understand more easily experiment situation, this device is preferably also provided with display screen 8, and described display screen 8 is connected with control unit, for showing real-time luminosity value and the temperature value of Experimental Area.Display screen 8 preferably adopts touch screen, can for man-machine interaction, carry out data query and setting simultaneously.Comparator, the programming that light source driving units is controlled and cooling piece driver element is controlled are prior art, repeat no more herein.

In to the research of retina cell photic damage, whether need the order of severity that under clear and definite equal conditions, illumination damages retina cell relevant with the radiation wave band of illumination, therefore the LED light source in described LED area source 2 is selected the LED lamp of different colours as required.Consider the integrity that will keep equipment, if use the LED lamp of single wave band, it is very inconvenient to change.In order to adapt to easily the research of different radiation wave band to retina cell damage, the light sending due to white light emitting diode (white light LEDs) has comprised visible ray, near-infrared band and nearultraviolet rays wave band, brightness is high, this installs described LED area source 2 and selects white led lamps, and the position near LED area source 2 is provided with elongated slot seam 9 on described camera bellows 1 sidewall, described camera bellows 1 inwall and elongated slot stitch 9 relative positions and are provided with guide groove, described elongated slot seam 9 and the cooperation of guide groove intermediate gap are inserted with optical filter 10, optical filter 10 is parallel with LED area source 2, can utilize like this optical filter 10 these optics to choose required radiation wave band.Such as inserting blue color filter 10, the light that white light LEDs sends is by blue color filter 10, ejaculation be a branch of blue light, and green glow, HONGGUANG are few, most ofly by optical filter 10, have been absorbed.By this structure, arrange, reach in the situation that not changing LED, realize the quantitative study to the damage of retina cell to the illumination of different-waveband under identical light intensity.