CN103719075A - Isolated organ preserving device and preserving method thereof - Google Patents
- ️Wed Apr 16 2014
CN103719075A - Isolated organ preserving device and preserving method thereof - Google Patents
Isolated organ preserving device and preserving method thereof Download PDFInfo
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- CN103719075A CN103719075A CN201410016261.2A CN201410016261A CN103719075A CN 103719075 A CN103719075 A CN 103719075A CN 201410016261 A CN201410016261 A CN 201410016261A CN 103719075 A CN103719075 A CN 103719075A Authority
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- 239000000463 material Substances 0.000 claims abstract description 13
- 238000004321 preservation Methods 0.000 claims abstract description 4
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- 239000000523 sample Substances 0.000 claims description 54
- 238000003860 storage Methods 0.000 claims description 33
- 238000001514 detection method Methods 0.000 claims description 31
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- 238000012545 processing Methods 0.000 claims description 20
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
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Abstract
The invention relates to an isolated organ preserving device and a preserving method thereof, and belongs to the technical field of medical equipment. The isolated organ preserving device is structurally characterized in that continuous mechanical irrigation and membrane oxygenation are adopted, an isolated organ continuously obtains nutrients and the oxygen supply, a cell metabolite is removed in time, and a cell microenvironment is better maintained, so that the function normality is ensured. The isolated organ preserving device disclosed by the invention can be used for directly contacting the organ perfusate and the organ perfusion instrument by adopting a disposable consumable material sterilized through epoxyethane, thereby meeting the requirement for sterility of a system. Besides, the isolated organ preserving device disclosed by the invention can be used for judging the consumption of the nutrients contained in the organ perfusate and the gathering degree of metabolic wastes by monitoring the pH value of the organ perfusate in real time and can be used for abandoning the organ perfusate after a certain standard is achieved and replacing the fresh organ perfusate, thereby further ensuring the possibility of long-time preservation; the isolated organ preserving device can be used for evaluating the damage condition of the isolated organ by monitoring the front and back perfusion pressure of the isolated organ in real time and converting the perfusion resistance by combining a flow number.
Description
Technical field
The present invention relates to a kind of organ save set and store method, belong to Medical Devices technical field.
Background technology
Organ transplant is the major progress of 20th century physianthropy science, give multiple whole latter stage organ disease treatment bring hope.At present, the various organ transplants that has carried out in the whole world have exceeded 700,000 examples (inferior).Along with the development of surgical technic and the appearance of neotype immunosuppressant, the survival rate of organ transplant significantly improves, and waits for that patient's quantity of accepting to transplant increases year by year.In order to alleviate the contradiction of organ isolated organ shortage, after cardiac arrest, contributing (DCD) becomes the vitals source of organ transplant gradually.Therefore, the clinical Techniques of preserving to organ has proposed requirements at the higher level.DCD organ is because there is inevitable warm ischemia time in it, and this time is called " no-touch time ".Therefore the metaboilic level of organ inner tissue cell cannot be at once because hypothermia perfusion reduces, and a large amount of metabolites are assembled.Tradition low temperature adds preserves the method that immersion bubble is preserved, and cell still maintains the metabolism of reduced levels, and nutriment consumes and metabolic waste local gathering in organ gradually, thereby causes intraorganic cell in stress situation, accelerates the apoptosis of cell.After transfer operation completes, blood supply is restored, and a large amount of oxygen is carried and entered in isolated organ by red blood cell, inevitably causes respiratory burst, causes serious ischemical reperfusion injury.Therefore, tradition is soaked store method has strict demand for the conserving time limit of organ, and organ preserves the oxygen in stage and the exhaustion of nutriment will further increase the weight of damage, has a strong impact on prognosis.Seek to provide raw material and the substrate of cellular metabolism, the circulation environment of excretion metabolism product seems and is even more important in time again.On the other hand, at present because the particularity of organ donation is with uncertain, preoperative preparation time of organ transplant is short.And owing to contributing organ, especially some rare organ shortage of joining type, many patients are in waiting time death.Therefore, if can extend as far as possible the time that organ is preserved, be conducive to clinician and improve as far as possible preoperative every inspection, contribute to select most suitable organ transplant acceptor simultaneously, guarantee to greatest extent surgical effect.Existing organ save set cannot meet actual needs.
Summary of the invention
The object of the invention is the defect existing for prior art, propose a kind of device and store method of preserving isolated organ, for isolated organ provides raw material and the substrate of cellular metabolism, and can timely excretion metabolism product.
The present invention is technical solution problem by the following technical programs: a kind of device of preserving isolated organ, by the equipment of Circuits System control and the consumptive material being arranged in equipment, formed, in described equipment, be provided with temperature controller and organ and preserve groove, the both sides that described organ is preserved groove connect the first detection groove and the second detection groove by pipeline respectively, described first detects groove connects spareslots by pipeline, described second detects groove connects oxygenate groove by pipeline, described spareslots and oxygenate groove pass through pipeline connection, on described the first detection groove, there is the first pressure detecting probe interface, on described the second detection groove, there is temperature measuring probe interface, pH value detection probe interface and the second pressure detecting probe interface, connecting line between described each groove is provided with peristaltic pump, described equipment electrical connection central processing unit, described central processing unit respectively with display screen, positioner is connected with power supply, described pipeline has joint, connect consumptive material, described consumptive material comprises that being placed in described organ preserves the organ storage bag in groove, be placed in described first and detect first in groove and the second detection groove, the second sampling bag, described the first sampling bag contains the first pressure detecting probe, described the second sampling bag contains the second pressure detecting probe, temperature measuring probe and pH detection probe, described each probe is electrically connected with described central processing unit, in described spareslots, be provided with two interfaces, settle respectively discarded organ perfusion's liquid collecting bag and standby organ perfusion's liquid storage bag, in described oxygenate groove, settle membrane oxygenator.
Device of the present invention is by 1) to maintain cellular metabolism local microenvironment stable to reach to continue machinery perfusion, supplements nutrient, takes away refuse; 2) membrane type oxygenate supplemental oxygen, to reach the oxygen supply that maintains isolated organ on suitable reduced levels, avoids the acidic materials that anaerobic metabolism excessively causes to assemble; 3) low temperature is preserved, and procedural intensification, to avoid organ to recover the intensification suddenly after blood flow, completes pre-adaptation processing, further reduces damage; 4) by the organ perfusion's liquid drainage that reaches discarded standard to spareslots, and brand-new organ perfusion's liquid of laying in spareslots is supplemented to circulating system, further guarantee the objects such as the long-distance transportation of organ and long-time preservation.
Described equipment of the present invention has outer wall and inwall, filling deionized water between described outer wall and inwall, and the temperature of described deionized water is by described temperature controller control, and described temperature controller is electrically connected with central processing unit; Described joint is standard Luer joint.Deionized water continues circulation between inside and outside wall, and by temperature controller, adjusting temperature range is 0-40 degree, maintains the constant temperature of whole system.
In described organ storage bag, be provided with perfusion pipeline, the internal diameter of described perfusion pipeline is 2-10mm, and external diameter is 3-12mm.Can coordinate replacing according to the perfusion blood vessel of different size.
In described organ storage bag, be provided with secretion collecting pipe, meet liver, kidney organ's excretion needs.
The internal diameter of described pipeline is 3-10mm.
The interface of described spareslots is provided with three-way device.
Between described power supply and central processing unit, be provided with accumulator.
The store method that the present invention further provides the device of preserving isolated organ, comprises the following steps:
Step 1, by filling organ perfusion liquid in organ storage bag, put into and need the isolated organ preserved, perfusion pipeline is connected with needing the blood vessel pouring on organ, and fixes with silk thread ligation;
Step 2, by organ storage bag put into organ preserve groove, be connected with described the first sampling bag, and further connect successively through the pipeline of peristaltic pump, discarded perfusion liquid collecting bag, standby organ perfusion's liquid storage bag, membrane oxygenator, through pipeline, the second sampling bag and the perfusion pipeline of peristaltic pump;
After step 3, connection, the first and second sampling bags are inserted respectively to the detection groove of its correspondence, corresponding with it each detection probe detection probe interface is connected, will discard perfusion liquid collecting bag, standby organ perfusion's liquid storage bag is inserted spareslots, and membrane oxygenator is inserted to oxygenate groove;
Step 4, switch on power, by display screen setup parameter, complete preservation.
In described step 1, while preserving liver, kidney organ, bile duct, ureter are connected with described secretion collection conduit, and fix with silk thread ligation.
Method of the present invention further comprises step 5, changes perfusion liquid, open the three-way device being communicated with discarded perfusion liquid collecting bag, liquid waste in pipeline is introduced to discarded perfusion liquid collecting bag, open again the three-way device being communicated with standby organ perfusion's liquid storage bag, standby organ perfusion's liquid, by peristaltic pump suction line, is completed to replacing.
Consumptive material in the present invention is oxirane disinfection, disposable use, directly contact with organ with organ perfusion's liquid, guaranteed the aseptic requirement of circulating system inside, pressure detecting, temperature detection and pH value detection probe have been integrated, to complete the quality control to organ perfusion's liquid, when reaching discarded standard simultaneously, can new organ perfusion's liquid be discharged and be supplemented to old organ perfusion's liquid gradually by three-port structure, to guarantee the stable of perfusion system chemistry and biological property.In addition, the membrane oxygenator that medical disposable material is integrated simultaneously can guarantee the partial pressure of oxygen that filling system is certain, by liquid dissolve by oxygen infusion to organ inside with guarantee oxygen supply.
Instrument has following functions: 1) store organ and organ perfusion's liquid; 2) by temperature control system, guarantee the stability of filling system temperature, and can complete pre-adaptation process by procedural slow rewarming, avoid the organ respiratory burst that rewarming causes rapidly, alleviate damage; 3) by peristaltic pump, provide the power of organ perfusion's liquid circulation, the flow of liquid is according to the weight of organ and front and back injection pressure is poor calculates; 4) integrate oxygen supply system, import and export and plug into the oxygen of medical disposable material, for membrane type oxygenate provides possibility; 5) by central processing unit, integrate indices, and Real-Time Monitoring situation is reflected on touching display screen; 6) touching display screen can complete the setting to instrument parameters simultaneously, and with temperature control system and the flow control system of crossing central processing unit control instrument; 7) by GPS assembly, complete the monitoring of instrument position, assist control centre to understand the travel position of organ; 8) by accumulator, guarantee that instrument still can normally be worked on the basis without external power supply.
The invention has the beneficial effects as follows: extend the isolated organ holding time, be conducive to clinician and improve as far as possible preoperative every inspection, contribute to select most suitable organ transplant acceptor simultaneously, guarantee to greatest extent surgical effect.
Accompanying drawing explanation
Fig. 1 is the structural representation of one embodiment of the invention.
Fig. 2 is the structural representation of consumptive material in Fig. 1.
Embodiment
Embodiment
The device of the present embodiment has and maintains machinery and pour into and control injection pressure and flow, the features such as Real-Time Monitoring perfusion liquid validity, as shown in Figure 1, structure comprises: outer wall 1, inwall 2, organ are preserved groove 3, temperature controller 4, pressure detecting probe interface 5, detection groove 6, peristaltic pump 7, spareslots 8, oxygenate groove 9,
peristaltic pump10, peristaltic pump 11, central processing unit 12, touching display screen 13, GPS terminal 14, power supply 15, accumulator 16, detected groove 17, temperature measuring probe interface 18, pH value detection probe interface 19, pressure detecting probe interface 20.
As shown in Figure 2, in the present embodiment, medical disposable material used comprises
organ storage bag21, standard Luer (luer)
joint22,
pressure detecting probe23,
sampling bag24,
standard luer joint25, connecting
line26, discarded perfusion
liquid collecting bag27,
standard luer joint28, three-
way device29, three-
way device30,
standard luer joint31, standby organ perfusion's
liquid storage bag32,
standard luer joint33,
standard luer joint34,
membrane oxygenator35,
standard luer joint36,
standard luer joint37, connecting
line38,
standard luer joint39,
sampling bag40,
temperature measuring probe41, pH
value detection probe42,
pressure detecting probe43,
standard luer joint44,
perfusion pipeline45,
secretion collection conduit46.
Wherein, the tube wall that organ perfusion's liquid is flowed through, for double-deck, comprises outer wall 1 and inwall 2, and between two-layer filling deionized water with conduction temperature.Between outer wall 1 and inwall 2 are two-layer, the deionized water temperature of filling is controlled by temperature controller 4, and the lasting pump pressure by peristaltic pump continues circulation at outer wall 1 and inwall 2 between two-layer, to maintain whole pipe-line system constant temperature.Temperature controller 4, is connected with central processing unit 12 by circuit, and the temperature setting according to touching display screen 13 and observed temperature adjusting.Thermoregulator scope is 0-40 ℃, and has procedural slow lifting/lowering temperature and rapid lifting/lowering temperature function.Isolated organ is obtaining and pre-perfused organ's perfusion liquid is placed in the
organ storage bag21 of pre-filling organ perfusion liquid.In
organ storage bag21, have
perfusion pipeline45, its bore can be changed according to selected perfusion blood vessel difference within the scope of internal diameter 2-10mm, external diameter 3-12mm.According to actual needs, in
organ storage bag21, be provided with secretion collection conduit 46.After
perfusion pipeline45 is connected with isolated organ with
secretion collection conduit46,
organ storage bag21 is placed in organ and preserves in groove 3, is connected subsequently by
standard luer joint22 with sampling bag 24.The built-in
pressure detecting probe23 of
sampling bag24, the pressure of Real-Time Monitoring efferent tract.After the opposite side of
sampling bag24 is connected with downstream connecting
line26 by
standard luer joint25, is positioned over and detects in groove 6,
pressure detecting probe23 is connected with pressure detecting probe interface 5, and is connected with central processing unit 12 by circuit, records outflow tract pressure.The internal diameter of connecting
line26, between 3-10mm, through peristaltic pump 7, is realized forward flowing of liquid in pipeline by its pump pressure.The liquid flow of the peristaltic pump 7 scrolling realization flow per minute by pump head roller between 200-2000ml.The downstream tandem three-
way device29 of connecting
line26, is connected with discarded perfusion
liquid collecting bag27 by standard luer joint 28.The downstream tandem three-
way device30 of connecting
line26, is connected with standby organ perfusion's
liquid storage bag32 by
standard luer joint31 simultaneously.Discarded perfusion
liquid collecting bag27 and standby organ perfusion's
liquid storage bag32 are all placed in spareslots 8.The downstream of connecting
line26 is also connected with
membrane oxygenator35 by accurate luer joint 33.
Membrane oxygenator35 enters 33 for organ perfusion's liquid by standard luer joint, by standard luer joint, flows out 37, and oxygen enters by
standard luer joint34, by
standard luer joint36,
discharges.Membrane oxygenator35, after being connected, is placed in oxygenate groove 9 with connecting
line26 and connecting
line38, guarantees its stability.Connecting
line38, through
peristaltic pump10, realizes forward flowing of liquid in pipeline by its pump pressure.
Peristaltic pump10, the liquid flow of the scrolling realization flow per minute by pump head roller between 200-
2000ml.Connecting line38, is connected with
sampling bag40 by standard luer joint 39.
Sampling bag40 includes
temperature measuring probe41, pH
value detection probe42 and
pressure detecting probe43, and is connected with
perfusion pipeline45 by
standard luer joint44, completes perfusion liquid-circulating.
Temperature measuring probe41, is connected with temperature measuring probe interface 18, detects in real time perfusion liquid temperature, by circuit, testing result is transferred to central processing unit 12, and shows in real time observed temperature by touching display screen 13.PH
value detection probe42, is connected with pH value detection probe interface 19, detects in real time perfusion liquid pH value, by circuit, testing result is transferred to central processing unit 12, and shows in real time actual measurement pH value by touching display screen 13.
Pressure detecting probe43 is connected with pressure detecting probe interface 20, detects in real time perfusion liquid pressure, by circuit, testing result is transferred to central processing unit 12.Central processing unit 12, the flow setting according to touching display screen 13, the pump head velocity of rotation of control peristaltic pump 7 and
peristaltic pump10, realizes the fluid flow between 200-2000ml per minute; Receive the data of
temperature measuring probe41, and the temperature setting according to touching display screen 13, temperature controller 4 inner heats controlled or the duty of the original paper that freezes; Receive the data of pH
value detection probe42, and will be by touching display screen 13 real-time display device official perfusion liquid pH values, be beneficial to determine whether to change organ perfusion liquid; Receive the data of
pressure detecting probe43 and
pressure detecting probe23, and according to both data difference, and the flow that sets of touching display screen 13, converse perfusion resistance, be beneficial to assess isolated organ perfusion state.By GPS terminal 14 real-time monitoring instrument positions, and communicate by letter with control centre, the convenient isolated organ of grasping instrument and preserve is dynamic.By power supply 15, provide power.Power supply 15, by transformer, is applicable to 12-20V direct current, and the situations such as 110V and 220V alternating current, can charge for accumulator 16 when providing power for device.The charging voltage of accumulator 16 is 12-20V direct current, 110V and 220V alternating current, discharge voltage is 12V direct current, and when power supply 15 does not connect for instrument provides power.
During use, complete after In situ perfusion method, organ is cut and is weighed, and just puts into afterwards the
organ storage bag21 of pre-filling organ perfusion liquid, and
perfusion pipeline45 is connected and is fixed with silk thread ligation with mechanical Perfused vessel selected on isolated organ.For liver, kidney and other organs, also need bile duct, ureter etc. be connected with
secretion collection conduit46 and fix with silk thread ligation.The caliber size of
perfusion pipeline45 and
secretion collection conduit46 can be changed according to the concrete condition of organ, adopts standard Luer joint to be connected with perfusion pipe-line system, guarantees universality.Subsequently,
organ storage bag21 is placed into organ and preserves in groove 3, and be connected with the
first sampling bag24, and be further connected with the connecting
line26 that passes peristaltic pump 7 successively, be connected with discarded perfusion
liquid collecting bag27, standby organ perfusion's
liquid storage bag32,
membrane oxygenator35, and be further connected with the connecting
line38 through
peristaltic pump10, be connected with the
second sampling bag40, perfusion pipeline 45.After having connected,
sampling bag24,
sampling bag40 inserted respectively and detect groove 6, detect in groove 17, and by
pressure detecting probe23 be connected with pressure detecting probe interface 5,
temperature measuring probe41 is connected with temperature measuring probe interface 18, pH
value detection probe42 is connected with pH value detection probe interface 19, pressure detecting is popped one's head in 43 is connected with pressure detecting probe interface 20; And
membrane oxygenator35 is inserted in oxygenate groove 9, and external oxygen channel is connected with
standard Luer joint36, discharge duct is connected with
standard Luer joint34, completes gas circulation; Further will discard perfusion
liquid collecting bag27, standby organ perfusion's
liquid storage bag32 is inserted in spareslots 8, completes the installation of whole system.
By power supply 15 or accumulator 16, for instrument moves, provide power, start is also set parameters by touching display screen 13.These parameters comprise filling temperature, flow etc., are shown in parameter on touching display screen 13 simultaneously and comprise perfusion liquid observed temperature, pH value, perfusion resistance, instrument GPS positional information and the power supply 15 that poor via front and back injection pressure and flow rate calculation obtains or accumulator 16 working conditions etc.
The replacing of perfusion liquid realizes by following operation: open three-
way device29, the liquid waste for the treatment of in connecting
line26 is introduced to discarded perfusion
liquid collecting bag27, open three-
way device30, the attraction providing by
peristaltic pump10 sucks perfusion pipe-line system by the fresh organ perfusion's liquid in standby organ perfusion's
liquid storage bag32, has completed replacing.Simultaneously, as the perfusion of need longer time, the discarded perfusion
liquid collecting bag27 of filling discarded perfusion liquid can be abandoned, connect empty standby organ perfusion's
liquid storage bag32, and new standby organ perfusion's
liquid storage bag32 of filling fresh organ perfusion's liquid is connected to the circulatory system.
The oxygenate of perfusion liquid is realized by following operation: complete after connection, medical oxygen enters in the inner core of
membrane oxygenator35 by
standard Luer joint36, and sees through oxygenation membrane disperse to outer sleeve, is dissolved in organ perfusion's liquid.
Except above-mentioned enforcement, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of requirement of the present invention.
Claims (10)
1. preserve the device of isolated organ for one kind, by the equipment of Circuits System control and the consumptive material being arranged in equipment, formed, it is characterized in that: in described equipment, be provided with temperature controller and organ and preserve groove, the both sides that described organ is preserved groove connect the first detection groove and the second detection groove by pipeline respectively, described first detects groove connects spareslots by pipeline, described second detects groove connects oxygenate groove by pipeline, described spareslots and oxygenate groove pass through pipeline connection, on described the first detection groove, there is the first pressure detecting probe interface, on described the second detection groove, there is temperature measuring probe interface, pH value detection probe interface and the second pressure detecting probe interface, connecting line between described each groove is provided with peristaltic pump, described equipment electrical connection central processing unit, described central processing unit respectively with display screen, positioner is connected with power supply, described pipeline has joint, connect consumptive material, described consumptive material comprises that being placed in described organ preserves the organ storage bag in groove, be placed in described first and detect first in groove and the second detection groove, the second sampling bag, described the first sampling bag contains the first pressure detecting probe, described the second sampling bag contains the second pressure detecting probe, temperature measuring probe and pH detection probe, described each probe is electrically connected with described central processing unit, in described spareslots, be provided with two interfaces, settle respectively discarded organ perfusion's liquid collecting bag and standby organ perfusion's liquid storage bag, in described oxygenate groove, settle membrane oxygenator.
2. preserve according to claim 1 the device of isolated organ, it is characterized in that: described equipment has outer wall and inwall, filling deionized water between described outer wall and inwall, the temperature of described deionized water is by described temperature controller control, and described temperature controller is electrically connected with central processing unit; Described joint is standard Luer joint.
3. the device of preserving according to claim 1 isolated organ, is characterized in that: in described organ storage bag, be provided with perfusion pipeline, the internal diameter of described perfusion pipeline is 2-10mm, and external diameter is 3-12mm.
4. the device of preserving according to claim 3 isolated organ, is characterized in that: in described organ storage bag, be provided with secretion collecting pipe.
5. the device of preserving according to claim 1 isolated organ, is characterized in that: the internal diameter of described pipeline is 3-10mm.
6. the device of preserving according to claim 1 isolated organ, is characterized in that: the interface of described spareslots is provided with three-way device.
7. the device of preserving according to claim 1 isolated organ, is characterized in that: between described power supply and central processing unit, be provided with accumulator.
8. the store method of preserving according to claim 1 the device of isolated organ, comprises the following steps:
Step 1, by filling organ perfusion liquid in organ storage bag, put into and need the isolated organ preserved, perfusion pipeline is connected with needing the blood vessel pouring on organ, and fixes with silk thread ligation;
Step 2, by organ storage bag put into organ preserve groove, be connected with described the first sampling bag, and further connect successively through the pipeline of peristaltic pump, discarded perfusion liquid collecting bag, standby organ perfusion's liquid storage bag, membrane oxygenator, through pipeline, the second sampling bag and the perfusion pipeline of peristaltic pump;
After step 3, connection, the first and second sampling bags are inserted respectively to the detection groove of its correspondence, corresponding with it each detection probe detection probe interface is connected, will discard perfusion liquid collecting bag, standby organ perfusion's liquid storage bag is inserted spareslots, and membrane oxygenator is inserted to oxygenate groove;
Step 4, switch on power, by display screen setup parameter, complete preservation.
9. the store method of preserving according to claim 8 the device of isolated organ, is characterized in that: in described step 1, while preserving liver, kidney organ, bile duct, ureter are connected with described secretion collection conduit, and fix with silk thread ligation.
10. preserve according to claim 8 the store method of the device of isolated organ, it is characterized in that: also comprise step 5, change perfusion liquid, open the three-way device being communicated with discarded perfusion liquid collecting bag, liquid waste in pipeline is introduced to discarded perfusion liquid collecting bag, open again the three-way device being communicated with standby organ perfusion's liquid storage bag, standby organ perfusion's liquid, by peristaltic pump suction line, is completed to replacing.
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Cited By (17)
* Cited by examiner, † Cited by third party| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104207857A (en) * | 2014-09-05 | 2014-12-17 | 浙江大学 | Control perfusion system and control perfusion method for ischemia of lower extremity |
| CN104430302A (en) * | 2014-12-04 | 2015-03-25 | 宋涂润 | Normal-temperature perfusion system for storing human organs |
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| CN104430302A (en) * | 2014-12-04 | 2015-03-25 | 宋涂润 | Normal-temperature perfusion system for storing human organs |
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| CN106689119A (en) * | 2017-02-17 | 2017-05-24 | 南京鼓楼医院 | Isolated organ in vitro perfusion preservation device |
| CN108719271A (en) * | 2017-04-13 | 2018-11-02 | 南方医科大学珠江医院 | Isolated organ Perfusion preservation movement system |
| CN107897169A (en) * | 2017-11-03 | 2018-04-13 | 四川大学华西医院 | In vitro organ oxygenation perfusion device |
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| CN110122474B (en) * | 2019-04-26 | 2021-08-10 | 浙江机电职业技术学院 | Human body broken limb protection box based on non-contact sensing control perfusion technology |
| CN110122474A (en) * | 2019-04-26 | 2019-08-16 | 浙江机电职业技术学院 | Human body broken limb protection box based on non-contact sensing control perfusion technology |
| CN110568873A (en) * | 2019-08-26 | 2019-12-13 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | On-line monitoring system of isolated organ perfusion pH |
| CN110463690A (en) * | 2019-08-26 | 2019-11-19 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Isolated organ perfusion device |
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| CN110669669A (en) * | 2019-11-05 | 2020-01-10 | 西安交通大学医学院第一附属医院 | A modular three-dimensional perfusion cell culture system |
| CN112273370A (en) * | 2020-09-27 | 2021-01-29 | 中山大学附属第一医院 | An intelligent isolated organ perfusion regulation device and method |
| CN112088875A (en) * | 2020-09-28 | 2020-12-18 | 四川大学华西医院 | Organ preservation device with adjustable solution oxidation-reduction potential and implementation method thereof |
| CN112088875B (en) * | 2020-09-28 | 2023-07-25 | 四川大学华西医院 | An organ preservation device with adjustable oxidation-reduction potential of solution and its realization method |
| CN116369310A (en) * | 2023-06-02 | 2023-07-04 | 中山大学附属第一医院 | Portable uninterrupted blood flow organ perfusion device and use method thereof |
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Effective date of registration: 20191025 Address after: 210000 Zhongdan Ecological Life Science Industrial Park, No. 3-1, xinjinhu Road, Jiangbei new district, Nanjing City, Jiangsu Province Patentee after: Jiangsu saigu Cell Engineering Research Institute Co., Ltd Address before: 210000 Jinling century garden, Jiangsu, Nanjing 168-502 Patentee before: Lv Ling |
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