CN108251330A - Complex micro organism fungicide for administering soil pollution and its preparation method and application - Google Patents

The invention discloses a kind of complex micro organism fungicide, including the following raw material component：Japanese pseudomonad culture, Comamonas testosteroni culture, microbacterium culture and De Shi acidovorax facilis cultures；The present invention to various bacteria culture mixed fermentation by being made complex micro organism fungicide, to in soil because the organic substance residues such as nitrobenzene compound formed when insecticide or pesticide has effective degradation, while the mixed culture of special strain and multiple bacteria compound fermentation provide a kind of simple and practicable preparation method.

Complex micro organism fungicide for administering soil pollution and its preparation method and application

Technical field

It is dirty for administering soil the present invention relates to a kind of microbial bacterial agent and its preparation method and application more particularly to one kind Complex micro organism fungicide of dye and its preparation method and application.

Background technology

Nitrobenzene compounds are widely used in the production nitro of pesticide, dyestuff, explosive, rubber and other chemical products Benzene-like compounds are widely used, and environment can be entered by waste water and gas, also can because transport and production process in contingency and The improper disposition of memory tank, and largely enter environment, the nitrobenzene class pollutant in environment mainly includes nitrobenzene, nitroxyl chloride Benzene, nitrotoleune, nitrophenol, nitroaniline and nitrobenzoic acid etc., most of nitrobenzene compounds have chemical property The characteristics of stable, high toxicity and easy biological concentration, is put into the screen priority pollutants in US Gov Env Protection Agency and China In list.

In the prior art, the restorative procedure of nitrobenzene compounds pollution mainly includes Physical, chemical method and bioanalysis； Physical mainly adsorbs pollutant using porous resin and activated carbon etc.；Chemical method mainly eliminates dirt using oxidation reaction Contaminate object；Bioanalysis come degradation of contaminant, finally makes pollutant mineralising mainly using the engineering bacteria of domestication or structure.With change The advantages of method is compared with Physical, biological treatment is that expense is low, does not easily cause secondary pollution, can farthest reduce dirt The concentration of object is contaminated, is had a wide range of application.Meanwhile the soil containing nitrobenzene compounds is often that multiple pollutant coexists, and is utilized Synergistic effect and microorganism between microorganism can remove multiple pollutant, accelerate nitre simultaneously to the Co metabolism effect of substrate The degradation of based compound.Therefore, with the extensive use and development of biological reinforcing technology and technique for gene engineering, in prevention nitro In terms of the environmental pollution of benzene-like compounds, biological treatment has broad application prospects.

But there are the treatment effect of p-nitrophenyl class compound is poor, and makes for composite microbial bacteria of the prior art Standby process is complicated, and thalline is easy to run off, so as to cause the wasting of resources.

Invention content

Goal of the invention：The first object of the present invention is to provide a kind of complex micro organism fungicide.

The second object of the present invention is to provide the preparation method of the complex micro organism fungicide.

The third object of the present invention is to provide the application of the complex micro organism fungicide.

Technical solution：In order to achieve the above-mentioned object of the invention, the present invention provides a kind of complex micro organism fungicide, including as follows Raw material components：Japanese pseudomonad culture, Comamonas testosteroni culture, microbacterium culture and the training of De Shi acidovorax facilis Support object.

Wherein, Japanese pseudomonad culture, Comamonas testosteroni culture, microbacterium culture and De Shi food acid The ratio of bacterium culture is 1:(2~3):(1.5~2.5):(3~4).

Preferably, the Japanese pseudomonad culture is Japanese aeruginosa atcc 33616 or ATCC33660 cultures Object；The Comamonas testosteroni culture is 39523 culture of ATCC 700441 or ATCC；The microbacterium culture For 31001 cultures of ATCC；The De Shi acidovorax facilis culture is 49664 culture of DSM 50403 or ATCC.

Further, the raw material components of complex micro organism fungicide further include bacillus subtilis culture, fixed nitrogen actinomyces Culture, hydrogenlike silicon ion culture and candidiasis culture.

Wherein, bacillus subtilis culture is 21556 cultures of bacillus subtilis ATCC；Fixed nitrogen actinomyces are cultivated Object is 33255 cultures of fixed nitrogen actinomyces ATCC；Hydrogenlike silicon ion culture is hydrogenlike silicon ion ATCC 49419, ATCC 17023rd, 33575 cultures of ATCC；Candidiasis culture is cultivated for candidiasis CICC 33050, NYNU 14772 Object.

Preferably, Japanese pseudomonad culture, Comamonas testosteroni culture, microbacterium culture, De Shi foods Sour bacterium culture, bacillus subtilis culture, fixed nitrogen actinomyces culture, hydrogenlike silicon ion culture and candidiasis training Support object ratio be：1:(2~3):(1.5~2.5):(3~4):(2.5~3.5):(0.5~1):(1~1.5):(2~3).

A kind of preparation method of complex micro organism fungicide, includes the following steps：

(1) using mixed culture medium A to Japanese pseudomonad culture, Comamonas testosteroni culture, microbacterium Culture and De Shi acidovorax facilis culture are mixed to obtain mixed culture A by weight；

(2) nitrobenzene compound is added in the mixed culture A of step (1), the mixed culture B after being tamed；

(3) bacillus subtilis culture in the mixed culture B and raw material components after the domestication for obtaining step (2), Fixed nitrogen actinomyces culture, hydrogenlike silicon ion culture and candidiasis culture are inoculated into level-one training according to parts by weight respectively It supports in case, aeration culture obtains level-one bacterium solution, then level-one bacterium solution is inoculated into two level incubator and obtains two level bacterium solution；

(4) the two level bacterium solution for each strain for obtaining step (3) uniformly mixes, and bacterial suspension is made, and controls effectively living Bacterium number >=1.5 × 1010/g obtain the complex micro organism fungicide.

Wherein, in step (1), the acquisition pattern of each microbial strain culture is：Each strain in the raw material components is inoculated with In slant medium, expand culture respectively, obtain the culture of each strain；

Preferably, each slant medium and condition of culture are respectively：Japanese aeruginosa atcc 33616 or ATCC33660 cultures：Nutrient agar culture medium, pH 6.8 ± 0.2, cultivation temperature：28℃；Comamonas testosteroni 39523 culture of ATCC 700441 or ATCC：Nutrient agar (NA), pH 6.8 ± 0.2, cultivation temperature:30.0℃；Microbacterium 31001 cultures of ATCC：Nutrient agar, pH 6.8 ± 0.2, cultivation temperature：37℃；De Shi acidovorax facilis DSM 50403 or 49664 cultures of ATCC：Nutrient agar (NA), pH 6.8 ± 0.2, cultivation temperature:30.0℃；Bacillus subtilis ATCC 21556 cultures：Nutrient agar (NA) plus 1% potato starch, 6.8 ± 0.2 cultivation temperatures of pH：37℃；Fixed nitrogen actinomyces 33255 cultures of ATCC：Malt extract powder 130g/L, agar 15g/L, chloramphenicol 0.1g/L, pH 6.0 ± 0.2, cultivation temperature: 28.0℃；Hydrogenlike silicon ion ATCC 49419, ATCC 17023,33575 cultures of ATCC：Model Neil yeast agar culture Base, pH 7.0 ± 0.2, cultivation temperature:24.0℃；Candidiasis CICC 33050,14772 cultures of NYNU：YCM is cultivated Base, cultivation temperature：25℃.

In step (1), the mixed culture medium A includes the component of following concentration of component：8~12g/L of tryptone, soybean 10~20g/L of peptone, 2~5g/L of beef leaching object, H₃BO₃2~5g/L, NaH₂PO₄·H₂O2~4g/L, 5~8g/L of NaCl, fine jade Fat 10~20g/L and MnCl₂0.2~0.3g/L of 4H2O, pH value are 6.5~6.8.

Preferably, it is the culture for the mixed culture A that will be in exponential phase the step of the domestication in step (2) Nitrobenzene compound is added in base, respectively the secondary culture at 2~3 days, 4~6 days and 7~9 days, make strain density reach 4 × 106~5 × 106/ml, bacterium survival rate >=85%.

Further, it in step (3), is inoculated into level-one incubator, aeration culture obtains level-one bacterium solution, then by level-one bacterium Liquid be inoculated into two level incubator the specific steps are：The incubator of 5~8L is inoculated by 2~4% inoculum concentration of culture medium In, temperature is 20~25 DEG C, and pH value of solution is neutral, aeration culture 40h, then 5~8L level-one bacterium solutions are transferred in two level incubator, Obtain 20~30L of two level bacterium solution；Wherein, nutrient media components are：Corn flour 10g/L, 5 g/L of glucose, beancake powder 10g/L, fish Powder 7g/L, CaCO₃ 7g/L、(NH₄)₂SO₄ 10g/L、K₂HPO₄ 0.4g/L、 MgSO₄.7H₂O 0.3g/L and MnSO₄·H₂O 0.2g/L。

Preferably, in step (4), the preparation method of bacterial suspension：It is dilute that 3.0ml~5.0ml is drawn with 5.0ml suction pipes It releases liquid to add in the culture medium of each microbial strain culture, pressure-vaccum, washes lower lawn repeatedly；Then, washing lotion is moved to separately with 5.0ml suction pipes In one sterile test tube, mixed several seconds with motorized agitator or shaken on palm and struck several times, so that each microbial strain culture suspends Uniformly obtained multi strain co cultivation object suspension.

The content of present invention further includes a kind of application of complex micro organism fungicide in soil pollution is administered.

Further, application of the complex micro organism fungicide in soil pollution is administered is being administered for the complex micro organism fungicide Application in by nitrobenzene compound contaminated soil.

Advantageous effect：Compared with prior art, the present invention is compound micro- by the way that various bacteria culture mixed fermentation is made Bacteria agent, to effectively being dropped in soil because the organic substance residues such as nitrobenzene compound formed when insecticide or pesticide has Solution acts on, while the mixed culture of special strain and multiple bacteria compound fermentation provide a kind of simple and practicable preparation method.

Specific embodiment

Technical scheme of the present invention is described in detail below, unless otherwise specified, following raw materials according can be obtained from commercially available .

Embodiment 1

Raw material components：Japanese 33616 culture of aeruginosa atcc, Comamonas testosteroni ATCC 700441 are cultivated The ratio of 50403 culture of object, 31001 cultures of microbacterium ATCC and De Shi acidovorax facilis DSM is 1:2:1.5:3.

It prepares and applies：(1) Japanese pseudomonad culture, Comamonas testosteroni are trained using mixed culture medium A Object, microbacterium culture and De Shi acidovorax facilis culture is supported to be mixed to obtain mixed culture A, mixing training by weight Support the component that base A includes following concentration of component：Tryptone 8g/L, soya peptone 10g/L, beef leaching object 2g/L, H₃BO₃ 2g/ L、NaH₂PO₄·H₂O 2g/L, NaCl 5g/L, agar 10g/L and MnCl₂.4H₂0.2 g/L of O, pH value 6.5；

(2) nitrobenzene compound is added in the mixed culture A of step (1), the mixed culture B after being tamed, The step of domestication, adds nitrobenzene compound will to be in the culture medium of mixed culture A, respectively at 2 days, 4 days and 7 days Secondary culture makes strain density reach 4 × 10⁶A/ml, bacterium survival rate 85%.

(3) the mixed culture B after the domestication for obtaining step (2) is inoculated into level-one incubator, and aeration culture obtains Level-one bacterium solution, then level-one bacterium solution is inoculated into two level incubator.

(4) zymotic fluid that step (3) obtains uniformly is mixed, is made bacterial suspension, control living bacteria count 1.5 × 10¹⁰A/g obtains the complex micro organism fungicide.

Embodiment 2

Raw material components：Japanese 33660 culture of aeruginosa atcc, Comamonas testosteroni ATCC 39523 are cultivated The ratio of 49664 culture of object, 31001 cultures of microbacterium ATCC and De Shi acidovorax facilis ATCC is 1:2.5:2: 3.5.

It prepares and applies：(1) Japanese pseudomonad culture, Comamonas testosteroni are trained using mixed culture medium A Object, microbacterium culture and De Shi acidovorax facilis culture is supported to be mixed to obtain mixed culture A, mixing training by weight Support the component that base A includes following concentration of component：Tryptone 10g/L, soya peptone 15g/L, beef leaching object 3g/L, H₃BO₃ 4g/L、NaH₂PO₄·H₂O 3g/L, NaCl 7g/L, agar 15g/L and MnCl₂.4H₂0.25 g/L of O, pH value 6.7；

(2) nitrobenzene compound is added in the mixed culture A of step (1), the mixed culture B after being tamed, The step of domestication, adds nitrobenzene compound will to be in the culture medium of mixed culture A, respectively at 3 days, 5 days and 8 days Secondary culture makes strain density reach 4.5 × 10⁶A/ml, bacterium survival rate 90%；

(3) the mixed culture B after the domestication for obtaining step (2) is inoculated into level-one incubator, and aeration culture obtains Level-one bacterium solution, then level-one bacterium solution is inoculated into two level incubator；

(4) zymotic fluid that step (3) obtains uniformly is mixed, is made bacterial suspension, control living bacteria count for 2 × 10¹⁰A/g obtains the complex micro organism fungicide.

Embodiment 3

Raw material components：Japanese 33660 culture of aeruginosa atcc, Comamonas testosteroni ATCC 39523 are cultivated The ratio of 49664 culture of object, 31001 cultures of microbacterium ATCC and De Shi acidovorax facilis ATCC is 1:3:2.5:4.

It prepares and applies：(1) Japanese pseudomonad culture, Comamonas testosteroni are trained using mixed culture medium A Object, microbacterium culture and De Shi acidovorax facilis culture is supported to be mixed to obtain mixed culture A, mixing training by weight Support the component that base A includes following concentration of component：Tryptone 12g/L, soya peptone 20g/L, beef leaching object 5g/L, H₃BO₃ 5g/ L、NaH₂PO₄·H₂O 4g/L, NaCl 8g/L, agar 20g/L and MnCl₂.4H₂O 0.3g/L, pH value 6.8；

(2) nitrobenzene compound is added in the mixed culture A of step (1), the mixed culture B after being tamed, The step of domestication, adds nitrobenzene compound will to be in the culture medium of mixed culture A, respectively at 3 days, 6 days and 9 days Secondary culture makes strain density reach 5 × 10⁶A/ml, bacterium survival rate 90%；

(3) the mixed culture B after the domestication for obtaining step (2) is inoculated into level-one incubator, and aeration culture obtains Level-one bacterium solution, then level-one bacterium solution is inoculated into two level incubator；

(4) zymotic fluid that step (3) obtains uniformly is mixed, is made bacterial suspension, control living bacteria count 2.0 × 10¹⁰A/g obtains the complex micro organism fungicide.

Embodiment 4

Raw material components：Japanese 33660 culture of aeruginosa atcc, Comamonas testosteroni ATCC 39523 are cultivated Object, 31001 cultures of microbacterium ATCC, 49664 cultures of De Shi acidovorax facilis ATCC, bacillus subtilis ATCC 21556 are trained Support object, 33255 cultures of fixed nitrogen actinomyces ATCC, 49419 cultures of hydrogenlike silicon ion ATCC and candidiasis CICC The ratio of 33050 cultures is：1:2:1.5:3:2.5:0.5:1:2.

It prepares and applies：(1) Japanese pseudomonad culture, Comamonas testosteroni are trained using mixed culture medium A Object, microbacterium culture and De Shi acidovorax facilis culture is supported to be mixed to obtain mixed culture A, mixing training by weight Support the component that base A includes following concentration of component：Tryptone 8g/L, soya peptone 10g/L, beef leaching object 2g/L, H₃BO₃ 2g/ L、NaH₂PO₄·H₂O2g/L, NaCl 5g/L, agar 10g/L and MnCl₂.4H₂O 0.2g/L, pH value 6.5；

(2) nitrobenzene compound is added in the mixed culture A of step (1), the mixed culture B after being tamed, The step of domestication, adds nitrobenzene compound will to be in the culture medium of mixed culture A, respectively at 2 days, 4 days and 7 days Secondary culture makes strain density reach 4 × 10⁶A/ml, bacterium survival rate are 85%；

(3) bacillus subtilis culture in the mixed culture B and raw material components after the domestication for obtaining step (2), Fixed nitrogen actinomyces culture, hydrogenlike silicon ion culture and candidiasis culture are inoculated into level-one training according to parts by weight respectively It supports in case, aeration culture obtains level-one bacterium solution, then level-one bacterium solution is inoculated into two level incubator and obtains two level bacterium solution；

(4) zymotic fluid that step (3) obtains uniformly is mixed, bacterial suspension is made, control living bacteria count is 1.5 ×10¹⁰A/g obtains the complex micro organism fungicide.

Embodiment 5

Raw material components：Japanese 33616 culture of aeruginosa atcc, Comamonas testosteroni ATCC 700441 are cultivated Object, 31001 cultures of microbacterium ATCC, 50403 cultures of De Shi acidovorax facilis DSM, bacillus subtilis ATCC 21556 are trained Support object, 33255 cultures of fixed nitrogen actinomyces ATCC, 17023 cultures of hydrogenlike silicon ion ATCC and candidiasis NYNU The ratio of 14772 cultures is：1:2.5:2:3.5:3:0.75:1.25:2.5.

It prepares and applies：(1) Japanese pseudomonad culture, Comamonas testosteroni are trained using mixed culture medium A Object, microbacterium culture and De Shi acidovorax facilis culture is supported to be mixed to obtain mixed culture A, mixing training by weight Support the component that base A includes following concentration of component：Tryptone 10g/L, soya peptone 15g/L, beef leaching object 4g/L, H₃BO₃ 3g/ L、NaH₂PO₄·H₂O 3g/L, NaCl 7g/L, agar 15g/L and MnCl₂.4H₂O 0.25g/L, pH value 6.7；

(2) nitrobenzene compound is added in the mixed culture A of step (1), the mixed culture B after being tamed, The step of domestication, adds nitrobenzene compound will to be in the culture medium of mixed culture A, respectively at 2 days, 5 days and 8 days Secondary culture makes strain density reach 4.5 × 10⁶A/ml, bacterium survival rate are 90%；

(3) bacillus subtilis culture in the mixed culture B and raw material components after the domestication for obtaining step (2), Fixed nitrogen actinomyces culture, hydrogenlike silicon ion culture and candidiasis culture are inoculated into level-one training according to parts by weight respectively It supports in case, aeration culture obtains level-one bacterium solution, then level-one bacterium solution is inoculated into two level incubator and obtains two level bacterium solution；

(4) zymotic fluid that step (3) obtains uniformly is mixed, is made bacterial suspension, control living bacteria count 2.0 × 10¹⁰A/g obtains the complex micro organism fungicide.

Embodiment 6

Raw material components：Japanese 33616 culture of aeruginosa atcc, Comamonas testosteroni ATCC 700441 are cultivated Object, 31001 cultures of microbacterium ATCC, 50403 cultures of De Shi acidovorax facilis DSM, bacillus subtilis ATCC 21556 are trained Support object, 33255 cultures of fixed nitrogen actinomyces ATCC, 33575 cultures of hydrogenlike silicon ion ATCC and candidiasis NYNU The ratio of 14772 cultures is：1:3:2.5:4:3.5:1:1.5:3.

It prepares and applies：(1) Japanese pseudomonad culture, Comamonas testosteroni are trained using mixed culture medium A Object, microbacterium culture and De Shi acidovorax facilis culture is supported to be mixed to obtain mixed culture A, mixing training by weight Support the component that base A includes following concentration of component：Tryptone 12g/L, soya peptone 20g/L, beef leaching object 5g/L, H₃BO₃ 5g/ L、NaH₂PO₄·H₂O4g/L, NaCl 8g/L, agar 20g/L and MnCl₂.4H₂O 0.3g/L, pH value 6.8；

(2) nitrobenzene compound is added in the mixed culture A of step (1), the mixed culture B after being tamed, The step of domestication, adds nitrobenzene compound will to be in the culture medium of mixed culture A, respectively at 3 days, 6 days and 9 days Secondary culture makes strain density reach 5 × 10⁶A/ml, bacterium survival rate 93%；

(3) bacillus subtilis culture in the mixed culture B and raw material components after the domestication for obtaining step (2), Fixed nitrogen actinomyces culture, hydrogenlike silicon ion culture and candidiasis culture are inoculated into level-one training according to parts by weight respectively It supports in case, aeration culture obtains level-one bacterium solution, then level-one bacterium solution is inoculated into two level incubator and obtains two level bacterium solution；

(4) zymotic fluid that step (3) obtains uniformly is mixed, is made bacterial suspension, control living bacteria count 2.5 × 10¹⁰A/g obtains the complex micro organism fungicide.

Comparative example 1

Raw material components：21556 cultures of bacillus subtilis ATCC, 33255 cultures of fixed nitrogen actinomyces ATCC, class ball The ratio of red 33575 cultures of bacterium ATCC and 14772 cultures of candidiasis NYNU is 3.5:1:1.5: 3.

It prepares and applies：Other specific steps and implementation condition are same as Example 6.

Comparative example 2

Raw material components：700441 cultures of Comamonas testosteroni ATCC, 31001 cultures of microbacterium ATCC, moral 50403 cultures of family name's acidovorax facilis DSM, 21556 cultures of bacillus subtilis ATCC, fixed nitrogen actinomyces ATCC 33255 are cultivated The ratio of 14772 culture of object, 33575 cultures of hydrogenlike silicon ion ATCC and candidiasis NYNU is 3:2.5:4:3.5: 1:1.5:3.(lacking Japanese pseudomonad culture)

It prepares and applies：Other specific steps and implementation condition are same as Example 6.

Comparative example 3

Raw material components：Japanese 33616 culture of aeruginosa atcc, 31001 cultures of microbacterium ATCC, De Shi food acid 50403 cultures of bacterium DSM, 21556 cultures of bacillus subtilis ATCC, 33255 cultures of fixed nitrogen actinomyces ATCC, class The ratio of red 33575 cultures of bacterium ATCC of ball and 14772 cultures of candidiasis NYNU is： 1：2.5:4:3.5:1: 1.5:3.(lacking Comamonas testosteroni culture)

It prepares and applies：Other specific steps and implementation condition are same as Example 6.

Comparative example 4

Raw material components：Japanese 33616 culture of aeruginosa atcc, Comamonas testosteroni ATCC 700441 are cultivated Object, 50403 cultures of De Shi acidovorax facilis DSM, 21556 cultures of bacillus subtilis ATCC, fixed nitrogen actinomyces ATCC The ratio of 14772 culture of 33255 cultures, 33575 cultures of hydrogenlike silicon ion ATCC and candidiasis NYNU is：1: 3:4:3.5:1:1.5:3.(lacking microbacterium culture)

It prepares and applies：Other specific steps and implementation condition are same as Example 6.

Comparative example 5

Raw material components：Japanese 33616 culture of aeruginosa atcc, Comamonas testosteroni ATCC 700441 are cultivated Object, 31001 cultures of microbacterium ATCC, 21556 cultures of bacillus subtilis ATCC, fixed nitrogen actinomyces ATCC 33255 are trained The ratio for supporting object, 33575 cultures of hydrogenlike silicon ion ATCC and candidiasis NYNU 14772 culture is：1:3:2.5: 3.5:1:1.5:3.(lacking De Shi acidovorax facilis culture)

It prepares and applies：Other specific steps and implementation condition are same as Example 6.

Using the complex micro organism fungicide in above-described embodiment 1~6 and comparative example 1~5 to by nitrobenzene compound dirt Pollutant in the soil of dye is handled, by being detected to the nitrobenzene compound in soil, as a result such as table 1：

Influence of the complex micro organism fungicide of 1 different material component of table to nitrobenzene compound degradation rate in polluted soil

As shown in Table 1, it is red thin to only have bacillus subtilis culture, fixed nitrogen actinomyces culture, class ball for the explanation of comparative example 1 When bacterium culture and candidiasis culture act on polluted soil nitrobenzene compound, effect is smaller, about 9.54%；Comparative example 2~5 illustrate when the complex micro organism fungicide that act on soil lack respectively Japanese pseudomonad culture, When Comamonas testosteroni culture, microbacterium culture or De Shi acidovorax facilis cultures, pollutant nitrobenzene compound Degradation rate is no more than 40.57%；Examples 1 to 3 explanation only has bacillus subtilis culture, fixed nitrogen actinomyces culture, class When polluted soil, p-nitrophenyl compound can play for the red bacterial cultures of ball and candidiasis culture collective effect Good degradation, more than 72.44%；Especially, when Japanese pseudomonad culture, C testosteroni in embodiment 4~6 Pseudomonas culture, microbacterium culture, De Shi acidovorax facilis culture, bacillus subtilis culture, the culture of fixed nitrogen actinomyces Object, hydrogenlike silicon ion culture and candidiasis culture collective effect when polluted soil, p-nitrophenyl compound Degradation rate is excellent, minimum more than 80%；Wherein, the effect of 6 degrading nitrobenzene compound of embodiment is best, p-nitrophenyl chemical combination The degradation rate of object can reach 88.51%.It can be seen that the raw material components in complex micro organism fungicide in the present invention lack one Can not, considerable effect is played to administering one of soil pollutant nitrobenzene compound.