CN114804953A - Potassium fulvate composite seed dressing agent and preparation method and application thereof - Google Patents

The invention discloses a potassium fulvate composite seed dressing agent and a preparation method and application thereof, wherein the potassium fulvate composite seed dressing agent comprises the following components in percentage by concentration per 100ml of seed dressing agent concentrated solution: 29-40% of mineral source potassium fulvate, 5-20% of animal amino acid, 16-30% of photosynthetic bacteria, 16-30% of wood vinegar bacteria and the balance of water. According to the potassium fulvate composite seed dressing agent provided by the invention, when seeds are soaked, the germination rate can be obviously improved by adding the fulvic acid seed dressing agent, the production process of the potassium fulvate composite seed dressing agent is simple, the preparation and application processes are environment-friendly, and the prepared seed dressing agent can be widely applied to seed dressing, leaf surface irrigation and irrigation with water of various crops. The application of the potassium fulvate compound seed dressing agent can reduce the rice planting cost from the root, improve the seed utilization rate and improve the grain productivity.

一种黄腐酸钾复合拌种剂及其制备方法、应用A kind of potassium humate composite seed dressing agent and preparation method and application thereof

技术领域technical field

本发明公开涉及液体肥料技术领域，尤其涉及一种黄腐酸钾复合拌种剂及其制备方法、应用The invention discloses and relates to the technical field of liquid fertilizers, in particular to a potassium fulvic acid compound seed dressing agent and a preparation method and application thereof

背景技术Background technique

近几年随着水稻工厂化育秧技术的迅速发展，为了实施工厂化育秧，省工节本，且为秧苗生产创造适宜的环境条件，市场上对育苗拌种剂的需求逐步增加。提早播种育苗，增加有效积温、培育的秧苗素质好、秧苗抗逆能力强等要求，是实现水稻种子良种化、供秧商品化的有效途径，也是实现水稻生产全程机械化的关键环节。In recent years, with the rapid development of rice seedling raising technology in factories, in order to implement factory raising seedlings, save labor and cost, and create suitable environmental conditions for seedling production, the market demand for seedling dressing agents has gradually increased. Early sowing and raising of seedlings, increasing effective accumulated temperature, good quality of cultivated seedlings, and strong stress resistance of seedlings are an effective way to realize the improvement of rice seeds and the commercialization of seedling supply, and are also a key link to realize the mechanization of the whole process of rice production.

综上，提供一种复合拌种剂以提高水稻的育苗水平是非常有意义的。In conclusion, it is very meaningful to provide a compound seed dressing to improve the seedling level of rice.

发明内容SUMMARY OF THE INVENTION

鉴于此，本发明公开提供了一种黄腐酸钾复合拌种剂及其制备方法，以提升种子利用率及粮食产能等。In view of this, the present invention discloses and provides a potassium fulvic acid composite seed dressing agent and a preparation method thereof, so as to improve the utilization rate of seeds and grain production capacity.

本发明提供的技术方案，第一方面，本发明提供了一种黄腐酸钾复合拌种剂，按浓度百分比含量计，每100ml所述拌种剂浓缩液中包括：矿源黄腐酸钾29-40％，动物氨基酸5-20％，光合细菌16-30％，木醋菌16-30％，余量为水。According to the technical solution provided by the present invention, in the first aspect, the present invention provides a potassium fulvic acid composite seed dressing agent. In terms of concentration percentage, every 100ml of the seed dressing agent concentrate includes: mineral potassium fulvic acid 29 -40%, animal amino acids 5-20%, photosynthetic bacteria 16-30%, Acetobacter xylinum 16-30%, the balance is water.

优选地，按浓度百分比含量计，每100ml所述拌种剂浓缩液中包括：矿源黄腐酸钾29％，动物氨基酸5％，光合细菌16％，木醋菌16％，余量为水。Preferably, in terms of concentration percentage, each 100ml of the seed dressing concentrate includes: mineral potassium fulvic acid 29%, animal amino acid 5%, photosynthetic bacteria 16%, Acetobacter xylinum 16%, and the balance is water.

第二方面，本发明提供了一种黄腐酸钾复合拌种剂的制备方法，包括：Second aspect, the invention provides a kind of preparation method of potassium fulvic acid composite seed dressing agent, comprising:

选择并按照质量浓度百分含量比称取黄腐酸钾、动物氨基酸原粉、光合细菌及木醋液，将其充分搅拌溶解于去离子水中并发生螯合反应即得到所述黄腐酸钾复合拌种剂。Select and weigh potassium fulvic acid, animal amino acid raw powder, photosynthetic bacteria and wood vinegar liquid according to the mass concentration percentage ratio, fully stirring and dissolving them in deionized water and chelating reaction to obtain the potassium fulvic acid Compound seed dressing.

优选地，称取黄腐酸钾29g，动物氨基酸原粉5g，光合细菌溶液16ml,木醋液16ml,去离子水68ml。Preferably, weigh 29g of potassium humate, 5g of animal amino acid raw powder, 16ml of photosynthetic bacteria solution, 16ml of wood vinegar, and 68ml of deionized water.

最后本发明提供了上述一种黄腐酸钾复合拌种剂的应用，所述拌种剂与作物拌种、叶面及随水灌溉施用。Finally, the present invention provides the application of the above-mentioned potassium fulvic acid composite seed dressing agent, which is applied to crop seed dressing, foliage and water-irrigated application.

优选地，所述拌种剂与作物拌种时，拌种剂浓缩液与稀释液的混合体积比为1:100，得到的稀释后的拌种剂与水稻种搅拌混合，混合比为100g/L,混合后避光静置48h，充分吸涨催化后播种，发芽育苗温度为28-32℃。Preferably, when the seed dressing agent and crop seed dressing, the mixing volume ratio of the seed dressing agent concentrate and the diluent is 1:100, and the obtained diluted seed dressing agent is stirred and mixed with rice seeds, and the mixing ratio is 100g/ L. After mixing, let it stand in the dark for 48 hours, fully absorb and catalyze and then sow the seedlings. The temperature for germination and seedling cultivation is 28-32 °C.

本发明提供的一种黄腐酸钾复合拌种剂，当种子进行浸泡时，添加本黄腐酸类拌种剂可以使其发芽率显著提高，该黄腐酸钾复合拌种剂生产工艺简单，制备和施用过程对环境友好，制备的拌种剂可广泛应用于各种作物的拌种、叶面和随水灌溉施用。应用该黄腐酸钾复合拌种剂能够从根源上降低水稻种植成本，提升种子利用率，提高粮食产能。In the potassium fulvic acid compound seed dressing provided by the invention, when the seeds are soaked, adding the fulvic acid seed dressing can significantly improve the germination rate, and the production process of the potassium fulvic acid compound seed dressing is simple. , the preparation and application process are friendly to the environment, and the prepared seed dressing agent can be widely used in seed dressing, foliar surface and irrigation application of various crops. The application of the potassium fulvic acid compound seed dressing agent can reduce the cost of rice planting from the root, improve the utilization rate of seeds, and increase the grain production capacity.

应当理解的是，以上的一般描述和后文的细节描述仅是示例性和解释性的，并不能限制本发明的公开。It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not intended to limit the disclosure of the present invention.

具体实施方式Detailed ways

这里将详细地对示例性实施例进行说明。以下示例性实施例中所描述的实施方式并不代表与本发明相一致的所有实施方式。相反，它们仅是与如所附权利要求书中所详述的、本发明的一些方面相一致的系统的例子。Exemplary embodiments will be described in detail herein. The implementations described in the illustrative examples below are not intended to represent all implementations consistent with the present invention. Rather, they are merely examples of systems consistent with some aspects of the invention as recited in the appended claims.

为进一步提升种子利用率，提高粮食产能，首先本实施方案提供了一种黄腐酸钾复合拌种剂，按浓度百分比含量计，每100ml所述拌种剂浓缩液中包括：矿源黄腐酸钾29-40％，动物氨基酸5-20％，光合细菌16-30％，木醋菌16-30％，余量为水。黄腐酸也被称为“天然生根粉”。施用黄腐酸肥料可以促进种子萌发、植株生长、改善作物品质、增强抗逆性等，也可以改善土壤的理化性质，在改善土壤结构、增强土壤保水保肥性、调节土壤pH值等方面发挥着重要作用；还能增进氮、磷、钾的肥效，提高它们的利用率；另外施用黄腐酸产品后，因其能够对农药起到缓释增效的效果，可以适当减少农药使用量达到防治污染的效果。In order to further improve the utilization rate of seeds and increase the grain production capacity, first of all, this embodiment provides a potassium fulvic acid composite seed dressing agent, in terms of the concentration percentage content, each 100ml of the seed dressing agent concentrate includes: mineral source fulvic acid. Potassium 29-40%, animal amino acids 5-20%, photosynthetic bacteria 16-30%, Acetobacter xylinum 16-30%, and the balance is water. Fulvic acid is also known as "natural rooting powder". The application of fulvic acid fertilizer can promote seed germination, plant growth, improve crop quality, enhance stress resistance, etc. It can also improve the physical and chemical properties of soil, and play a role in improving soil structure, enhancing soil water and fertilizer retention, and adjusting soil pH. It can also improve the fertilizer efficiency of nitrogen, phosphorus and potassium, and improve their utilization rate; in addition, after the application of fulvic acid products, because of its slow-release and synergistic effect on pesticides, the amount of pesticides used can be appropriately reduced. The effect of pollution prevention.

按浓度百分比含量计，每100ml所述拌种剂浓缩液中包括：矿源黄腐酸钾29％，动物氨基酸5％，光合细菌16％，木醋菌16％，余量为水。In terms of concentration percentage, each 100ml of the seed dressing concentrate includes: mineral potassium fulvic acid 29%, animal amino acid 5%, photosynthetic bacteria 16%, Acetobacter xylinum 16%, and the balance is water.

本实施方案配方中的黄腐酸发挥螯合作用，与动物氨基酸发生氨化反应，激活活性氨；黄腐酸与木醋液酸性环境中发生水解，与黄腐酸共同固氮；光合细菌发酵液各种活性物质易打破种子休眠，起到催芽短提供种子发芽。将三种辅助试剂与黄腐酸混合配比，达到催芽功效，不仅降低农药农肥的污染效果，同时也减轻水稻田土壤碱化风险。经试验得出黄腐酸钾复合拌种剂成分比例为黄腐酸钾：动物氨基酸原粉：光合细菌：液木醋液：去离子水＝29:5:16:16:68，此比例有效提升水稻种发芽率。The fulvic acid in the formula of this embodiment plays a chelating role, undergoes ammoniation reaction with animal amino acids, and activates active ammonia; fulvic acid and wood vinegar are hydrolyzed in an acidic environment, and together with fulvic acid fix nitrogen; photosynthetic bacterial fermentation broth Various active substances are easy to break seed dormancy and play a role in accelerating germination to provide seed germination. The three auxiliary reagents are mixed with fulvic acid to achieve the effect of germination, which not only reduces the pollution effect of pesticides and fertilizers, but also reduces the risk of soil alkalization in paddy fields. The composition ratio of potassium fulvic acid compound seed dressing agent is obtained through experiments: potassium fulvic acid: raw animal amino acid powder: photosynthetic bacteria: liquid wood vinegar: deionized water = 29:5:16:16:68, this ratio is effective Improve the germination rate of rice seeds.

此外，本实施方案还提供了一种黄腐酸钾复合拌种剂的制备方法，包括：In addition, the present embodiment also provides a preparation method of a potassium fulvic acid composite seed dressing, comprising:

选择并按照质量浓度百分含量比称取黄腐酸钾、动物氨基酸原粉、光合细菌及木醋液，将其充分搅拌溶解于去离子水中并发生螯合反应即得到所述黄腐酸钾复合拌种剂。Select and weigh potassium fulvic acid, animal amino acid raw powder, photosynthetic bacteria and wood vinegar liquid according to the mass concentration percentage ratio, fully stirring and dissolving them in deionized water and chelating reaction to obtain the potassium fulvic acid Compound seed dressing.

称取黄腐酸钾29g，动物氨基酸原粉5g，光合细菌溶液16ml,木醋液16ml,去离子水68ml。Weigh 29g of potassium humate, 5g of animal amino acid raw powder, 16ml of photosynthetic bacteria solution, 16ml of wood vinegar, and 68ml of deionized water.

即拌种剂浓缩液制备方法：将29g黄腐酸钾，5g动物氨基酸原粉(山东君德生物科技有限公司，有效成分含量≥99％)，16ml光合细菌(泰州市富特思生物科技有限公司，主成分海洋红螺菌，红假单胞菌等)16ml木醋液(北京溪青春农业科技有限公司，有效成分含量≥70％),溶解于68ml去离子水中，其中各有效成分的质量百分含量分别为矿源黄腐酸钾29％，动物氨基酸5％，光合细菌16％，木醋菌16％，室温避光保存即可。The preparation method of the seed dressing concentrate: 29g potassium fulvic acid, 5g animal amino acid raw powder (Shandong Junde Biotechnology Co., Ltd., active ingredient content ≥ 99%), 16ml photosynthetic bacteria (Taizhou Futesi Biotechnology Co., Ltd. Company, the main components of marine Rhodospirillum, Rhodopseudomonas, etc.) 16ml wood vinegar (Beijing Xi Qingchun Agricultural Technology Co., Ltd., active ingredient content ≥ 70%), dissolved in 68ml deionized water, the quality of each active ingredient The percentages are respectively 29% of mineral potassium fulvic acid, 5% of animal amino acids, 16% of photosynthetic bacteria, and 16% of Acetobacter xylinum, which can be stored at room temperature in the dark.

上述拌种剂浓缩液中加入黄腐植酸钾中具有羧基、羰基、醇羟基、酚羟基等官能团，有较强的离子交换和吸附能力，能减少铵态氮损失，提高氮肥利用率。同时也黄腐植酸钾也具备改善土壤的理化性质，在改善土壤结构、增强土壤保水保肥性、调节土壤pH值等方面发挥着重要作用，还能增进氮、磷、钾的肥效，提高它们的利用率；The potassium humate added to the above-mentioned seed dressing concentrate has functional groups such as carboxyl group, carbonyl group, alcohol hydroxyl group, phenolic hydroxyl group, etc., and has strong ion exchange and adsorption capacity, which can reduce the loss of ammonium nitrogen and improve the utilization rate of nitrogen fertilizer. At the same time, potassium humate also has the ability to improve the physical and chemical properties of soil. It plays an important role in improving soil structure, enhancing soil water and fertilizer retention, and adjusting soil pH. utilization rate;

上述配方中加入的动物氨基酸与黄腐植酸钾吸附螯合后，并与其发生氨化反应，生成解离度很低的腐植酸铵盐，既可为作物提供NH⁴⁺，又减少氨的挥发，及增强了吸附离子及固氮能力，水稻等作物可直接吸收氨基酸态氮并达到很好的生长效果，减少氮素流失。木醋液中的醇类、酚类对水稻生长起调节作用,木醋液具有促进水解的作用，在本拌种剂中经水合反应成NH⁴⁺，与黄腐酸钾相配合固氮，减少耗能。土壤中NH⁴⁺很快被据研究黄腐植酸钾中羧基、羰基、醇羟基、酚羟基等官能团，有较强的离子交换和吸附能力，能减少铵态氮损失，提高氮肥利用率。光合细菌发酵液中含有多种生理活性物质，如叶绿素A、叶绿素B、类胡萝卜素、多种维生素、多种植物激素和核酸、水杨酸、多种氨基酸以及单细胞蛋白质等，这些物质能促进种子发芽，打破种子休眠，起到催芽短时间的作用，同时保证了大量元素的缓慢释放和充分利用，又保证了微量元素的稳效与长效。The animal amino acid added in the above formula is adsorbed and chelated with potassium fulvic acid, and undergoes an ammoniation reaction with it to generate ammonium humate with a very low degree of dissociation, which can not only provide NH ⁴⁺ for crops, but also reduce the volatilization of ammonia. , and enhance the adsorption ion and nitrogen fixation ability, rice and other crops can directly absorb amino acid nitrogen and achieve good growth effect, reduce nitrogen loss. The alcohols and phenols in the wood vinegar have a regulating effect on the growth of rice, and the wood vinegar has the effect of promoting hydrolysis. In this seed dressing agent, NH ⁴⁺ is reacted by hydration, and it is combined with potassium fulvic acid to fix nitrogen, reducing the energy consumption. According to research, NH ⁴⁺ in soil has been quickly identified as carboxyl, carbonyl, alcohol hydroxyl, phenolic hydroxyl and other functional groups in potassium humate, which has strong ion exchange and adsorption capacity, can reduce the loss of ammonium nitrogen and improve the utilization rate of nitrogen fertilizer. The fermentation broth of photosynthetic bacteria contains a variety of physiologically active substances, such as chlorophyll A, chlorophyll B, carotenoids, a variety of vitamins, a variety of plant hormones and nucleic acids, salicylic acid, a variety of amino acids and single-cell proteins, etc. Promote seed germination, break seed dormancy, and play a role in accelerating germination for a short time. At the same time, it ensures the slow release and full utilization of a large number of elements, and ensures the stable and long-term effect of trace elements.

最后，上述黄腐酸钾复合拌种剂的应用，将拌种剂与作物拌种、叶面及随水灌溉施用。另外施用黄腐酸产品后，因其能够对农药起到缓释增效的效果，可以适当减少农药使用量达到防治污染的效果。该拌种剂利用光合细菌发酵液的活性物质打破种子休眠，在木醋液提供的酸性水解环境中，利用黄腐酸与动物氨基酸充分进行氨化反应，充分螯合吸附作用，配合固氮，为植物提供了大量的直接吸收氨基酸态氮，减少铵态氮损失，同时也保证了大量元素的缓慢释放和充分利用，又保证了微量元素的稳定与长效，提高氮肥利用率。Finally, in the application of the above-mentioned potassium fulvic acid compound seed dressing agent, the seed dressing agent is applied with crop seed dressing, foliar surface and irrigation with water. In addition, after the application of fulvic acid products, because of its slow release and synergistic effect on pesticides, the use of pesticides can be appropriately reduced to achieve the effect of pollution control. The seed dressing uses the active substances of the photosynthetic bacteria fermentation broth to break the seed dormancy, and in the acidic hydrolysis environment provided by the wood vinegar, the fulvic acid and animal amino acids are used to fully carry out the ammoniation reaction, fully chelating and adsorbing, and cooperating with nitrogen fixation. Plants provide a large amount of direct absorption of amino acid nitrogen, reduce the loss of ammonium nitrogen, and at the same time ensure the slow release and full utilization of a large number of elements, and ensure the stability and long-term effect of trace elements, and improve the utilization rate of nitrogen fertilizer.

上述拌种剂与作物拌种时，取得所述黄腐酸钾复合拌种剂浓缩液，1:100比例混合使用。根据1:100比例，吸取黄腐酸钾复合拌种剂10ml制备成1L成伴种剂稀释，其中稀释液为去离子水。将此1L稀释液与100g水稻种搅拌混合均匀即浓缩液：稀释液：种子质量伴种剂施用体积质量比例为：1:100:10，避光静置48h，充分吸涨催化后播种，发芽育苗温度为28-32℃。When the above-mentioned seed dressing agent and crop seed dressing are used, the concentrated solution of the potassium fulvic acid composite seed dressing agent is obtained, and the mixture is used in a ratio of 1:100. According to the ratio of 1:100, absorb 10ml of potassium fulvic acid compound seed dressing agent to prepare 1L of seed companion agent for dilution, wherein the diluent is deionized water. Stir and mix this 1L diluent with 100g of rice seeds evenly, that is, the concentrated solution: diluent: seed quality with seed agent application volume-to-mass ratio: 1:100:10, let stand in the dark for 48h, fully absorb and catalyze, sow and germinate Seedling temperature is 28-32 ℃.

本实施方案采用实验室施用试验的方法，分别从单一配方中筛选最佳浓度组合成为复合伴种剂配方，并通过发芽率实验验证本发明的作用与功效；下面结合具体实施例对本发明作进一步说明，本发明的具体实施例和比较例中所用水均为蒸馏水，试验农作物选用水稻种子，施用方式为拌种喷洒。This embodiment adopts the method of laboratory application test, respectively selects the best concentration combination from a single formula to form a compound seed agent formula, and verifies the action and efficacy of the present invention through the germination rate experiment; the present invention is further described below with reference to specific examples. Note that the water used in the specific examples and comparative examples of the present invention is distilled water, and rice seeds are selected for the test crops, and the application method is seed dressing and spraying.

实施例1：Example 1:

为筛选出适合水稻发芽的黄腐酸钾溶液浓度。分别配置质量浓度为0.00％(对照组)，0.29％、0.33％、0.40％、0.50％，0.67％、0.1％的单一黄腐酸钾溶液，分别取30ml配置溶液，与水稻种子100粒搅拌混合均匀后，避光播种，实验重复三次，将发芽育苗温度为28-32℃，观察发芽情况计算平均发芽率，In order to screen out the concentration of potassium fulvic acid solution suitable for rice germination. Prepare a single potassium fulvic acid solution with a mass concentration of 0.00% (control group), 0.29%, 0.33%, 0.40%, 0.50%, 0.67%, 0.1%, respectively, take 30ml of the prepared solution and mix with 100 rice seeds. After uniformity, the seeds were planted in the dark, and the experiment was repeated three times.

筛选出适合水稻发芽的黄腐酸钾溶液浓度。结果如表1所示，黄腐酸溶液浓度为0.29％、0.33％时发芽率高于对照组。The concentration of potassium fulvic acid solution suitable for rice germination was screened out. The results are shown in Table 1. The germination rate was higher than that of the control group when the concentration of fulvic acid solution was 0.29% and 0.33%.

表1黄腐酸单配方筛选：Table 1 Fulvic acid single formula screening:

伴种剂配方Seed formulation 平均发芽率(％)Average germination rate (%) 误差error 黄腐酸钾0.0％(对照)Potassium humate 0.0% (control) 7272 1.241.24 黄腐酸钾0.29％Potassium humate 0.29% 7979 2.922.92 黄腐酸钾0.33％Potassium humate 0.33% 82.182.1 0.50.5 黄腐酸钾0.40％Potassium humate 0.40% 66.7766.77 1.561.56 黄腐酸钾0.50％Potassium humate 0.50% 62.5862.58 0.220.22 黄腐酸钾0.67％Potassium humate 0.67% 55.4455.44 1.971.97 黄腐酸1.00％Fulvic acid 1.00% 44.1944.19 1.551.55

实施例2：Example 2:

为筛选出适合水稻发芽的动物氨基酸溶液浓度。分别配置质量浓度为0.00％(对照组)，0.29％、0.05％、0.067％、0.1％，0.2％的单一动物氨基酸溶液，分别取30ml配置溶液，与水稻种子100粒搅拌混合均匀后，避光播种，实验重复三次，将发芽育苗温度为28-32℃，观察发芽情况计算平均发芽率，筛选出适合水稻发芽的动物氨基酸溶液浓度。结果如表2所示，动物氨基酸溶液浓度为0.05％、0.067％时发芽率高于对照组。In order to screen out the concentration of animal amino acid solution suitable for rice germination. Prepare single animal amino acid solution with mass concentration of 0.00% (control group), 0.29%, 0.05%, 0.067%, 0.1%, 0.2%, respectively, take 30ml of the prepared solution, stir and mix with 100 rice seeds, and protect from light. Sowing, the experiment was repeated three times, the temperature of germination and seedling was 28-32°C, the germination situation was observed to calculate the average germination rate, and the concentration of animal amino acid solution suitable for rice germination was screened. The results are shown in Table 2. When the concentration of animal amino acid solution is 0.05% and 0.067%, the germination rate is higher than that of the control group.

表2动物氨基酸单配方筛选：Table 2 animal amino acid single formula screening:

伴种剂配方Seed formulation 平均发芽率(％)Average germination rate (%) 误差error 动物氨基酸0.0％(对照)Animal amino acid 0.0% (control) 7272 1.241.24 动物氨基酸0.05％Animal Amino Acids 0.05% 75.1275.12 1.711.71 动物氨基酸0.067％Animal Amino Acids 0.067% 73.6273.62 0.50.5 动物氨基酸0.1％Animal Amino Acids 0.1% 62.2462.24 1.361.36 动物氨基酸0.2％Animal Amino Acids 0.2% 51.351.3 1.221.22

实施例3：Example 3:

为筛选出适合水稻发芽的光合细菌溶液浓度。分别配置质量浓度为0.00％(对照组)，0.16％、0.20％、0.25％、0.33％，0.2％的单一光合细菌溶液，分别取30ml配置溶液，与水稻种子100粒搅拌混合均匀后，避光播种，实验重复三次，将发芽育苗温度为28-32℃，观察发芽情况计算平均发芽率，筛选出适合水稻发芽的光合细菌溶液浓度。结果如表3所示，光合细菌溶液浓度为0.16％、0.20％时发芽率高于对照组。In order to screen out the concentration of photosynthetic bacteria solution suitable for rice germination. Prepare a single photosynthetic bacteria solution with a mass concentration of 0.00% (control group), 0.16%, 0.20%, 0.25%, 0.33%, and 0.2%, respectively, take 30ml of the prepared solution, stir and mix with 100 rice seeds, and protect from light. Sowing, the experiment was repeated three times, the germination temperature was 28-32°C, the germination situation was observed to calculate the average germination rate, and the concentration of photosynthetic bacteria solution suitable for rice germination was screened. The results are shown in Table 3. The germination rate was higher than the control group when the photosynthetic bacteria solution concentration was 0.16% and 0.20%.

表3光和细菌单配方筛选：Table 3 Light and Bacterial Single Recipe Screening:

伴种剂配方Seed formulation 平均发芽率(％)Average germination rate (%) 误差error 光合细菌0.0％(对照)Photosynthetic bacteria 0.0% (control) 7272 1.241.24 光合细菌0.16％Photosynthetic bacteria 0.16% 74.874.8 1.111.11 光合细菌0.20％Photosynthetic bacteria 0.20% 73.873.8 0.460.46 光合细菌0.25％Photosynthetic bacteria 0.25% 62.762.7 1.261.26 光合细菌0.33％Photosynthetic bacteria 0.33% 51.851.8 1.021.02

实施例4：Example 4:

为筛选出适合水稻发芽的木醋菌溶液浓度。分别配置质量浓度为0.00％(对照组)，0.16％、0.20％、0.25％、0.33％，0.2％的单一木醋菌溶液，分别取30ml配置溶液，与水稻种子100粒搅拌混合均匀后，避光播种，实验重复三次，将发芽育苗温度为28-32℃，观察发芽情况计算平均发芽率，筛选出适合水稻发芽的木醋菌溶液浓度。结果如表4所示，木醋菌溶液浓度为0.16％、0.20％时发芽率高于对照组。In order to screen out the concentration of Acetobacter xylinum suitable for rice germination. Prepare 0.00% (control group), 0.16%, 0.20%, 0.25%, 0.33%, and 0.2% single Acetobacter xylinum solution, respectively, take 30ml of the prepared solution, stir and mix with 100 rice seeds, avoid Light sowing, the experiment was repeated three times, the germination temperature was 28-32°C, the germination situation was observed to calculate the average germination rate, and the concentration of Acetobacter xylinum solution suitable for rice germination was screened. The results are shown in Table 4. When the concentration of Acetobacter xylinum solution was 0.16% and 0.20%, the germination rate was higher than that of the control group.

表4木醋菌单配方筛选Table 4 Single formula screening of Acetobacter xylinum

伴种剂配方Seed formulation 平均发芽率(％)Average germination rate (%) 误差error 木醋菌0.0％(对照)Acetobacter xylinum 0.0% (control) 7272 1.241.24 木醋菌0.16％Acetobacter xylinum 0.16% 76.1376.13 0.920.92 木醋菌0.20％Acetobacter xylinum 0.20% 75.1275.12 0.350.35 木醋菌0.25％Acetobacter xylinum 0.25% 61.5861.58 1.461.46 木醋菌0.33％Acetobacter xylinum 0.33% 61.2561.25 2.022.02

实施例5：Example 5:

为得到最佳发芽配方进行组合，筛选出以黄腐酸钾为主，动物氨基酸、光和细菌、木醋菌为辅的复合伴种剂配方。选用实施案例1中黄腐酸钾最佳浓度0.29％、0.33％，实施案例2中动物氨基酸溶液最佳浓度0.05％、0.067％，实施案例3中光合细菌溶液最佳浓度0.16％、0.20％，实施案例4中木醋菌溶液最佳浓度0.16％、0.20％进行组合，分别取30ml配置溶液，与水稻种子100粒搅拌混合均匀后，避光播种，实验重复三次，将发芽育苗温度为28-32℃，观察发芽情况计算平均发芽率，筛选出适合水稻发芽的黄腐酸复合伴种液浓度。结果如表5所示，黄腐酸钾0.29％+动物氨基酸0.005％+光合细菌0.17％+木醋菌0.17％的配方发芽率最高可达95.94％，远高于对照组。In order to obtain the best germination formula for combination, the compound seeding agent formula was screened out, which was mainly potassium humate, supplemented by animal amino acids, light and bacteria, and xylinum acetic acid bacteria. The optimal concentrations of potassium fulvic acid in Case 1 were 0.29% and 0.33%, the optimal concentrations of animal amino acid solutions in Case 2 were 0.05% and 0.067%, and the optimal concentrations of photosynthetic bacteria solutions in Case 3 were 0.16% and 0.20%. In Example 4, the optimal concentration of Acetobacter xylinum solution was 0.16% and 0.20%, and 30ml of the prepared solution was taken respectively. After stirring and mixing with 100 rice seeds, the seeds were planted in the dark. The experiment was repeated three times. At 32°C, the germination conditions were observed to calculate the average germination rate, and the concentration of the fulvic acid compound concomitant seed solution suitable for rice germination was screened. The results are shown in Table 5. The germination rate of the formula of potassium humate 0.29% + animal amino acid 0.005% + photosynthetic bacteria 0.17% + xylinum 0.17% can reach the highest germination rate of 95.94%, which is much higher than the control group.

表5黄腐酸复合伴种剂配方筛选：Table 5 fulvic acid compound seed preparation formula screening:

将上述实施例和比较例各个浓度溶液拌种及喷施比较得出最佳配比。通过矿源黄腐酸有机复合肥料催芽实验，在育苗期提升水稻发芽率，在优化后期施肥、追肥分配比例，降低育苗期种植孵化成本，提高种植效率。The best proportions were obtained by comparing the seed dressing and spraying of each concentration solution of the above examples and comparative examples. Through the germination experiment of mineral source fulvic acid organic compound fertilizer, the germination rate of rice is improved in the seedling stage, and the distribution ratio of fertilization and top dressing is optimized in the later stage, so as to reduce the cost of planting and hatching in the seedling stage, and improve the planting efficiency.

本领域技术人员在考虑说明书及实践这里公开的发明后，将容易想到本发明的其它实施方案。本申请旨在涵盖本发明的任何变型、用途或者适应性变化，这些变型、用途或者适应性变化遵循本发明的一般性原理并包括本发明未公开的本技术领域中的公知常识或惯用技术手段。说明书和实施例仅被视为示例性的，本发明的真正范围和精神由权利要求指出。Other embodiments of the invention will readily occur to those skilled in the art upon consideration of the specification and practice of the invention disclosed herein. This application is intended to cover any variations, uses or adaptations of the invention which follow the general principles of the invention and which include common knowledge or techniques in the technical field not disclosed by the invention . The specification and examples are to be regarded as exemplary only, with the true scope and spirit of the invention being indicated by the claims.