CN215688729U - Double-balloon fixable animal perfusion needle - Google Patents
- ️Tue Feb 01 2022
CN215688729U - Double-balloon fixable animal perfusion needle - Google Patents
Double-balloon fixable animal perfusion needle Download PDFInfo
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- CN215688729U CN215688729U CN202022994910.XU CN202022994910U CN215688729U CN 215688729 U CN215688729 U CN 215688729U CN 202022994910 U CN202022994910 U CN 202022994910U CN 215688729 U CN215688729 U CN 215688729U Authority
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- 238000002347 injection Methods 0.000 claims abstract description 45
- 239000011229 interlayer Substances 0.000 claims abstract description 38
- 238000002955 isolation Methods 0.000 claims abstract description 9
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- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
本实用新型提供了一种双球囊可固定动物灌注针，包括套管，套管内设置有穿刺针，套管外设置有一双球囊固定夹层；双球囊固定夹层包括两个可膨胀扩大的球囊，用以进行膨胀扩大并固定灌注针；套管固定于设置有空腔的套管底座，穿刺针连接于穿刺针底座，穿刺针和穿刺针底座通过隔离塞固定在套管底座内；套管底座的侧壁上还连接有一固定注射装置，固定注射装置与双球囊固定夹层连通。本实用新型提供的双球囊可固定动物灌注针，通过在灌注针上设置双球囊固定夹层，使灌注针主体固定在小鼠心脏心室壁上，可有效避免现有技术中灌注针在灌注过程中脱落，灌注针头刺破左心室内壁进入其他心腔，以及针刺部位针孔变大后导致心内压力不足，灌注剂泄露等情况。
The utility model provides a double-balloon fixable animal perfusion needle, which comprises a cannula, a puncture needle is arranged in the cannula, and a double-balloon fixing interlayer is arranged outside the casing; the double-balloon fixing interlayer comprises two expandable and expandable The balloon is used to expand and fix the perfusion needle; the cannula is fixed on the cannula base provided with the cavity, the puncture needle is connected to the puncture needle base, and the puncture needle and the puncture needle base are fixed in the cannula base through the isolation plug; A fixed injection device is also connected to the side wall of the casing base, and the fixed injection device is communicated with the double-balloon fixed interlayer. The double-balloons provided by the utility model can fix the animal perfusion needle. By arranging the double-balloon fixing interlayer on the perfusion needle, the main body of the perfusion needle is fixed on the ventricular wall of the mouse heart, which can effectively avoid the perfusion needle in the prior art. During the process, the perfusion needle pierced the inner wall of the left ventricle and entered other cardiac chambers, and the needle hole at the acupuncture site became larger, resulting in insufficient intracardiac pressure and leakage of perfusate.
Description
Technical Field
The utility model relates to the technical field of perfusion needles, in particular to a double-balloon fixable animal perfusion needle.
Background
In the field of medical scientific research, animal tissue can be well fixed by heart perfusion of animal specimens, the lightest autolysis degree and the best morphological and tissue antigen retention degree are ensured, and meanwhile, a certain experimental basis is provided for quantitative protein analysis, nucleic acid molecule detection and morphological observation of the microstructure of the tissue. In the conventional perfusion, a common syringe needle is used for puncturing from the left ventricle, then physiological saline or corrosive liquid (formalin and the like) or blood vessel perfusion agent is injected into the syringe for perfusion fixation or perfusion development, the perfusion liquid enters into all levels of arteries after entering an aorta through the left ventricle, and then returns to the right atrium through all levels of veins through capillary vessels of the whole body. During the perfusion process, the perfusion needle is very easy to fall off due to the heart movement or puncture the inner wall of the left ventricle, so that the perfusion liquid flows into the right ventricle to cause perfusion failure. Meanwhile, in some animal experiments, such as animal blood vessel perfusion experiments, due to the fact that the skin near a puncture needle hole is hardened due to formalin perfusion, the puncture needle hole is enlarged, pressure is reduced when contrast medium is injected, and the contrast medium leaks to cause perfusion failure of a small blood vessel at the far end. And the traditional perfusion mode has low perfusion success rate, needs two people to operate simultaneously, can only operate 1-2 animals at a time, has long experiment time consumption and low efficiency.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects of the prior art, the utility model provides the double-balloon fixable animal perfusion needle which is convenient and practical, can fix the perfusion needle on the wall of the left ventricle and is convenient for heart perfusion.
The utility model adopts the following technical scheme for solving the technical problems:
the utility model provides a double-balloon fixable animal perfusion needle, which comprises a sleeve, wherein a puncture needle is arranged in the sleeve, and a double-balloon fixed interlayer is arranged outside the sleeve;
the double-balloon fixing interlayer comprises two inflatable and expandable balloons for inflation and expansion and fixing the perfusion needle;
the sleeve is fixed on a sleeve base provided with a cavity and communicated with the cavity, the puncture needle is connected with the puncture needle base, and the puncture needle base are fixed in the sleeve base through an isolation plug; an isolation plug disposed in the cavity
The side wall of the sleeve base is also connected with a fixed injection device which is communicated with the double-balloon fixed interlayer.
Further, the double-balloon fixing interlayer comprises a first balloon close to the tip of the puncture needle and a second balloon far away from the tip of the puncture needle; the first sacculus and the second sacculus on the double-sacculus fixing interlayer are made of elastic materials.
Furthermore, the second balloon is connected with the first balloon through a connecting part, and one end of the first balloon, which is far away from the second balloon, is communicated with the fixed injection device through the bottom of the interlayer; the connecting portion and the bottom portion of the interlayer are not deformable.
Further, the fixed injection device comprises an injection part, and the material of the injection part is selected from one of rubber and silica gel.
Further, the material of the isolation plug is selected from one of rubber and silica gel.
Further, the fixed injection device is connected with the perfusion needle through the injection tube and is communicated with the double-balloon fixed interlayer.
Further, a closing clip is arranged on the injection tube.
Further, the distance between the two balloons is 0.1-5 mm.
Furthermore, one side of the puncture needle base is provided with a holding part for taking the puncture needle base and the puncture needle out of the cannula base and the cannula.
Furthermore, the holding part is provided with anti-skid convex points.
By adopting the technical scheme, compared with the prior art, the utility model has the following technical effects:
the double-balloon fixable animal perfusion needle provided by the utility model has the advantages that the double-balloon fixable interlayer is arranged on the perfusion needle, after the perfusion needle is penetrated into the wall of the left ventricle of the heart of a mouse, air or liquid is injected into the double-balloon fixable interlayer, so that the two balloons positioned inside and outside the wall of the ventricle are expanded and enlarged, the main body of the perfusion needle is fixed on the wall of the heart ventricle of the heart of the mouse, and the problems that the perfusion needle is easy to fall off in the perfusion process, or the perfusion penetrates the inner wall of the left ventricle of a needle head to enter other heart chambers, the intracardiac pressure is insufficient after a needle hole of a needle punching part is enlarged, and the perfusion fails due to the leakage of a perfusion agent in the prior art can be effectively avoided. Greatly improving the success rate of animal experiment perfusion.
Drawings
FIG. 1 is a schematic diagram of a double-balloon fixable animal injection needle according to an embodiment of the utility model;
FIG. 2 is a schematic view of the assembly of a closure clip and syringe in a double-balloon fixable animal injection needle provided in accordance with an embodiment of the present invention;
FIG. 3 is a schematic structural view of a double-balloon fixable animal injection needle (when the balloon is inflated) provided by an embodiment of the utility model;
FIG. 4 is a schematic view of a double-balloon fixable animal infusion needle provided in accordance with an embodiment of the present invention penetrating a wall of a mouse ventricle;
FIG. 5 is a schematic diagram of balloon inflation after a double-balloon fixable animal injection needle provided by an embodiment of the utility model is inserted into a ventricular wall of a mouse;
FIG. 6 is a schematic view of a double-balloon fixable animal perfusion needle provided in accordance with an embodiment of the present invention, taken out of a puncture needle and its base after the needle has penetrated into a mouse ventricle wall;
wherein the reference numerals are: 1-sleeve, 2-puncture needle, 3-double-balloon fixing interlayer, 31-first balloon, 32-second balloon, 33-connecting part, 34-interlayer bottom, 4-sleeve base, 5-puncture needle base, 51-holding part, 6-isolating plug, 7-fixing injection device, 71-injection part, 72-sealing clip and 8-injection tube.
Detailed Description
The utility model provides a double-balloon fixable animal perfusion needle.
The following detailed description of the present invention is provided in connection with the accompanying drawings for better understanding of the present invention, but not to limit the scope of the present invention.
As shown in figure 1, the utility model provides a double-balloon fixable animal perfusion needle, which comprises a
sleeve1, wherein a
puncture needle2 is arranged in the
sleeve1, and a double-balloon fixed
interlayer3 is arranged outside the
sleeve1;
the double-
balloon fixing interlayer3 comprises two inflatable and expandable balloons for inflating and expanding and fixing the perfusion needle;
the double-
balloon fixing interlayer3 comprises a
second balloon32 close to the tip of the
puncture needle2 and a
first balloon31 far away from the tip of the
puncture needle2; the
first balloon31 and the
second balloon32 on the double-
balloon fixing interlayer3 are made of elastic materials. The
second balloon32 is connected with the
first balloon31 through a connecting
part33, and one end of the
first balloon31 far away from the
second balloon32 is communicated with the fixed injection device 7 through an
interlayer bottom part34; the
connection portion32 and the
interlayer base34 are not deformable. The
first balloon31 and the
second balloon32 in the double
balloon fixing interlayer3 can be inflated and enlarged by injecting gas or liquid into the double
balloon fixing interlayer3, and the shapes of the connecting
part32 and the
interlayer bottom part34 are kept unchanged.
The
sleeve1 is fixed on a
sleeve base4 provided with a cavity and is communicated with the cavity; the
puncture needle2 is fixed on a
puncture needle base5, and the
puncture needle base5 is fixed in a
sleeve base4 through an
isolation plug6; the
isolating plug6 is arranged in the cavity of the
sleeve base4; one side on the
puncture needle base5 is provided with a
holding part51 for taking the
puncture needle base5 and the
puncture needle2 out of the
cannula base4 and the
cannula1, and the
holding part51 is provided with anti-skid convex points, so that the taking out of the
puncture needle base5 and the
puncture needle2 is easier through the anti-skid convex points.
The material of the
isolation plug6 is selected from one of rubber and silicone. The
puncture needle2 can be taken out from the
sleeve1 and the
isolating plug6 through the
puncture needle base5, and the
puncture needle base5 is also taken out from the
sleeve base4, at the moment, the
isolating plug6 can be automatically closed after the
puncture needle2 is pulled out because the
isolating plug6 is made of rubber or silica gel. After the
puncture needle2 is pulled out, normal saline or perfusate can be injected into the
sleeve1 through the
isolating plug6, and the isolating plug can be automatically closed after the completion of primary perfusion, so that the normal saline or the perfusate is prevented from flowing out of the isolating plug.
The automatic recovery of the seal by pulling out the needle after the rubber or
silicone septum6 has been pierced by the needle is well known in the art and will not be described herein.
The side wall of the
sleeve base4 is also connected with a fixed injection device 7, and the fixed injection device 7 is communicated with the double-balloon
fixed interlayer3.
As shown in fig. 3, the fixed injection device 7 includes an
injection part71, and the material of the
injection part71 is selected from one of rubber and silicone. The fixed injection device 7 is connected with the perfusion needle through an
injection tube8 and is communicated with the double-
sacculus fixing interlayer3. Gas or liquid can be injected through the
injection part71, and the gas or liquid flows into the double-
balloon fixing interlayer3 through the
injection pipe8, so that the
first balloon31 and the
second balloon32 are expanded, and the perfusion needle is fixed on the ventricular wall of the mouse.
As shown in FIG. 2, a
closure clip72 is provided on the
syringe8. The
injection part71 is made of one of rubber and silica gel, after gas or liquid is injected through the
injection part71, an injection needle for injecting the gas or the liquid is pulled out, the
injection part71 can be automatically closed, at the moment, the
injection tube8 can be squeezed into a thin gap of the
closing clamp72, the
closing clamp72 can close the
injection tube8, and the double-
balloon fixing interlayer3 is prevented from leaking air by matching with the material of the
injection part71.
In a preferred embodiment of the utility model, the distance between the two balloons is 0.1-5 mm. Namely, the length of the connecting
part33 between the
first balloon31 and the
first balloon32 is 0.1-5 mm, the distance is selected according to the body type of the perfused mouse and the thickness of the ventricular wall of the left ventricle, and the perfusion needle can be fixed on the ventricular wall after the double-
balloon fixing interlayer3 is expanded.
As shown in fig. 4 to 6, a method for using a double-balloon fixable animal perfusion needle according to an embodiment of the present invention includes:
firstly, according to the heart perfusion and blood vessel perfusion modeling standard of a mouse, splitting the thoracic cavity of the mouse, exposing the apex of the heart, finding the position of a left ventricle, inserting a double-balloon perfusion needle from the left ventricle through a
puncture needle2 and a
sleeve1, and stopping needle insertion after breakthrough feeling occurs;
then, injecting gas or liquid into the double
balloon fixing interlayer3 through the
injection part71 of the fixing injection device 7 to expand the
first balloon31 and the
second balloon32, and fixing the perfusion needle body on the ventricular wall of the mouse through the squeezing action of the
first balloon31 and the
second balloon32;
then, the
puncture needle2 is pulled out through the
pinching part51 of the
puncture needle base5, the
cannula1 and the double-balloon fixed interlayer are left in the left ventricle, the
isolating plug6 is automatically closed at the moment, the injector is inserted from the
isolating plug6, physiological saline is injected into the left ventricle through the cavity of the
cannula base4 and the
cannula1, meanwhile, the opening of the right auricle of the heart of the mouse is formed, the flow rate of the infusion apparatus is adjusted to be consistent with the blood pumping speed of the heart at the moment, the effluent liquid of the right auricle of the heart of the mouse is clarified and bright, the tail and the four limbs become white and lose the blood color, and the infusion of the physiological saline is considered to be finished;
and finally, pulling out the normal saline injector, replacing corrosive fixing liquid such as formalin and the like, inserting the corrosive fixing liquid into the
isolating plug6 for perfusion, and keeping the perfusion needle on the wall of the heart chamber of the mouse to ensure the stability of the circulating pressure of the mouse body until the materials are obtained.
The heart puncture hole can be well plugged by the extrusion of the double saccules, so that the perfusion needle cannot fall off from the wall of the heart chamber in the perfusion process; the perfusion needle is fixed on the ventricular wall by the double balloons in the perfusion process, so that the perfusion failure caused by the fact that the perfusion needle is further inserted into the inner ventricular wall of the heart of the mouse to enter the right ventricle without paying attention in the perfusion process is avoided; and the extrusion of two sacculums makes the heart chamber maintain higher pressure all the time, is difficult to appear stationary liquid refluence or reveal from the puncture hole, makes animal experiment perfusion's success rate and efficiency increase substantially.
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications or alterations to this practice will occur to those skilled in the art and are intended to be within the scope of this invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (10)
1.一种双球囊可固定动物灌注针，其特征在于，包括套管(1)，所述套管(1)内设置有穿刺针(2)，所述套管(1)外设置有一双球囊固定夹层(3)；1. A double-balloon fixable animal perfusion needle is characterized in that, comprising a cannula (1), a puncture needle (2) is provided in the cannula (1), and a puncture needle (2) is provided outside the cannula (1) A pair of balloons to fix the dissection (3); 所述双球囊固定夹层(3)包括两个可膨胀扩大的球囊，用以进行膨胀扩大并固定所述灌注针；The double-balloon fixation interlayer (3) includes two balloons that can be expanded and expanded, and used to expand and fix the perfusion needle; 所述套管(1)固定于设置有空腔的套管底座(4)并与所述空腔连通；所述穿刺针(2)固定于穿刺针底座(5)，所述穿刺针底座(5)通过隔离塞(6)固定在所述套管底座(4)内；所述隔离塞(6)设置于所述空腔内；The cannula (1) is fixed on a cannula base (4) provided with a cavity and communicates with the cavity; the puncture needle (2) is fixed on the puncture needle base (5), and the puncture needle base ( 5) be fixed in the casing base (4) by an isolation plug (6); the isolation plug (6) is arranged in the cavity; 所述套管底座(4)的侧壁上还连接有一固定注射装置(7)，所述固定注射装置(7)与所述双球囊固定夹层(3)连通。A fixed injection device (7) is also connected to the side wall of the sleeve base (4), and the fixed injection device (7) communicates with the double-balloon fixed interlayer (3). 2.根据权利要求1所述的灌注针，其特征在于，所述双球囊固定夹层(3)包括靠近所述穿刺针(2)尖端的第二球囊(32)和远离所述穿刺针(2)尖端的第一球囊(31)；所述第一球囊(31)和第二球囊(32)为弹性材料。2. The perfusion needle according to claim 1, wherein the double-balloon fixed interlayer (3) comprises a second balloon (32) close to the tip of the puncture needle (2) and a second balloon (32) away from the puncture needle (2) The first balloon (31) at the tip; the first balloon (31) and the second balloon (32) are elastic materials. 3.根据权利要求2所述的灌注针，其特征在于，所述第二球囊(32)和所述第一球囊(31)之间通过连接部(33)连接，所述第一球囊(31)远离所述第二球囊(32)的一端通过夹层底部(34)与所述固定注射装置(7)连通；所述连接部(33)和所述夹层底部(34)不可变形。3. The perfusion needle according to claim 2, wherein the second balloon (32) and the first balloon (31) are connected by a connecting portion (33), and the first balloon The end of the balloon (31) away from the second balloon (32) communicates with the fixed injection device (7) through the interlayer bottom (34); the connecting part (33) and the interlayer bottom (34) are not deformable . 4.根据权利要求1所述的灌注针，其特征在于，所述固定注射装置(7)包括注射部(71)，所述注射部(71)的材质选自橡胶和硅胶中的一种。4. The perfusion needle according to claim 1, wherein the fixed injection device (7) comprises an injection part (71), and the material of the injection part (71) is selected from one of rubber and silicone. 5.根据权利要求1所述的灌注针，其特征在于，所述隔离塞(6)的材料选自橡胶和硅胶中的一种。5. The perfusion needle according to claim 1, characterized in that, the material of the isolation plug (6) is selected from one of rubber and silicone. 6.根据权利要求1所述的灌注针，其特征在于，所述固定注射装置(7)通过注射管(8)连接所述灌注针，并与所述双球囊固定夹层(3)连通。6 . The perfusion needle according to claim 1 , wherein the fixed injection device ( 7 ) is connected to the perfusion needle through an injection tube ( 8 ) and communicated with the double-balloon fixed interlayer ( 3 ). 7 . 7.根据权利要求6所述的灌注针，其特征在于，所述注射管(8)上设置有一封闭夹(72)。7. The perfusion needle according to claim 6, wherein a closing clip (72) is provided on the injection tube (8). 8.根据权利要求1所述的灌注针，其特征在于，两个所述球囊之间的距离为0.1～5mm。8 . The perfusion needle according to claim 1 , wherein the distance between the two balloons is 0.1˜5 mm. 9 . 9.根据权利要求1所述的灌注针，其特征在于，所述穿刺针底座(5)上的一侧设置有捏持部(51)，用以将所述穿刺针底座(5)和穿刺针(2)从所述套管底座(4)和套管(1)中取出。9. The perfusion needle according to claim 1, characterized in that, a gripping portion (51) is provided on one side of the puncture needle base (5) for connecting the puncture needle base (5) to the puncture needle base (5). The needle (2) is removed from the cannula base (4) and cannula (1). 10.根据权利要求9所述的灌注针，其特征在于，所述捏持部(51)上设置有防滑凸点。10. The perfusion needle according to claim 9, characterized in that, anti-skid bumps are provided on the gripping portion (51).
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