CN2418882Y - In vitro pulmonary circulation and perfusion device - Google Patents

The utility model relates to a pulmonary circulation and perfusion device in vitro, composed of a respiration aerating system, a blood circulating system and a constant temperature system. The utility model is characterized in that an oxygen-removing device inside the blood circulating system is composed of a foaming board, a foaming chamber, a defoaming and filtering chamber and a blood-storing chamber and is arranged between a blood-storing groove and a blood pump. The oxygen-removing device can convert arterial blood formed by pulmonary perfusion in vitro into venous blood and is used for repeating the pulmonary perfusion in vitro. The device of the utility model has the advantages of physiological perfusion accordance, satisfiability for long-time experimental requirements, less blood-supply experimental animals, low experimental expenses, and reliability for experimental results.

Pulmonary circulation perfusion new equipment exsomatizes

This utility model belongs to the medical experiment device, mainly proposes a kind of stripped pulmonary circulation perfusion new equipment.

Lung transplantation successfully is applied to treat lung disease and pulmonary vascular disease in whole latter stage, and preserving quality for lung is the key link of lung transplantation success.When medical circle screening both at home and abroad at present and evaluation lung store method, mostly adopt the lung perfusion experiment device that exsomatizes.This type of device has 2 basic demands, and the first will meet the physiology perfusion, promptly with the stripped lung of venous blood perfusion; It two is that requirement experiment can be kept certain hour, and too short experimental period can not correctly be estimated lung and preserve.The lung perfusion device that exsomatizes at present has two types: first kind is disposable perfusion device, promptly adopts the disposable perfusion of the venous blood lung that exsomatizes.The method meets physiological requirement, but the blood volume of laboratory animal self can not satisfy the requirement of long-time perfusion, must gather venous blood from other laboratory animals, therefore wastes laboratory animal, increases experimental expenses.Second kind is the circulation perfusion device, and promptly the venous blood perfusion exsomatizes and to become arterial blood behind the lung, the reuse arterial blood circulation perfusion lung that exsomatizes.Though the method can satisfy long period of experiments and reduce the requirement of laboratory animal expense, but do not meet physiological requirement with the stripped lung of arterial blood perfusion, thereby experimental result is unreliable (consults paper: 1.The effect of PGE1 andtemperature on lung function following preservation.Ueno T.Transplantation, 1991, Vol 52, No4,626-630.2. rat separates lung perfusion Preparation of model and application.Gao Yuqi, etc.The Chinese Pharmacological circular, 1991 the 7th volume the 6th phase 474-477 pages or leaves.3．Hemodilution?reduces?early?reperfusion?injury?in?ExVivo?rabbit?lung?preservation?model.Puskas?JD.Ann?Thorac?Surg，1994，vol?57，731-735)。

The purpose of this utility model is to propose a kind of stripped pulmonary circulation perfusion new equipment, this device has the advantage of disposable perfusion device and circulation perfusion device simultaneously concurrently, make it both meet physiological requirement, experimental result accurately and reliably, perfusion and reduce the laboratory animal expense for a long time again.

For achieving the above object, this utility model has been taked following technical scheme: this utility model is made up of respiratory ventilation system, blood circulation and constant temperature system three parts; The respiratory ventilation system is made up of respirator, airway and pressure monitoring mouth, is used for the breathing of lung; Blood circulation is made up of stripped lung, blood storage groove, blood pump, connection emissary vein, blood sampling mouth, is used for the lung perfusion that exsomatizes; Constant temperature system is made up of constant temperature lung box, thermostat, water pump, heat exchanger and water circulating pipe, is used for guaranteeing that experiment blood and stripped lung are in temperature constant state.This utility model is provided with deaerator in blood circulation, its effect is arterial blood to be handled form venous blood.Deaerator is made up of foaming plate, foaming chamber, froth breaking and filter chamber and blood storage chamber.Deaerator is connected between blood storage groove and the blood pump, and promptly the foaming chamber of deaerator is communicated with blood storage groove, and its blood storage chamber is communicated with blood pump.

This utility model is to have installed deaerator additional in the stripped pulmonary circulation perfusion device of prior art, thereby taken into account the advantage of circulation perfusion device and disposable perfusion device, overcome the shortcoming of the two, reached long-time perfusion, reduce experimental expenses and meet physiological requirement, experimental result purpose accurately.

Accompanying drawing 1 is this utility model structural representation.

In conjunction with the accompanying drawings this utility model embodiment is illustrated:

As shown in Figure 1, the stripped pulmonary circulation perfusion device of this utility model is made up of respiratory ventilation system, blood circulation and constant temperature system three parts; The respiratory ventilation system is made up of respirator 1-1, airway 1-2 and pressure monitoring mouth 1-3; Blood circulation is made up of stripped lung 2-1, blood storage groove 2-2, blood pump 2-4, connection emissary vein 2-5, blood sampling mouth 2-6; Constant temperature system is made up of constant temperature lung box 3-1, thermostat 3-2, water pump 3-3, heat exchanger 3-4 and water circulating pipe 3-5; More than adopt the structure of the existing pulmonary circulation perfusion device that exsomatizes.This utility model is provided with deaerator 2-3 in blood circulation.Deaerator is made up of foaming plate 2-3-1, foaming chamber 2-3-2, froth breaking and the 2-3-3 of filter chamber and blood storage chamber 2-3-4; Deaerator is connected between blood storage groove 2-2 and the blood pump 2-4, and promptly the foaming chamber 2-3-2 of deaerator is communicated with blood storage groove 2-2, and its blood storage chamber 2-3-4 is communicated with blood pump 2-4.The operation principle of this part is: become arterial blood by oxygenate behind the stripped lung 2-1 of venous blood perfusion, be pooled to blood storage groove 2-2, enter deaerator 2-3 through emissary vein 2-5.In deaerator, form venous blood after the foaming of arterial blood process, froth breaking, the filtration.Venous blood is by the extruded emissary vein 2-5 stripped lung of perfusion again of crossing of blood pump 2-4, and formation meets physiological circulation perfusion, reaches experiment purpose.In the deaerator, foaming plate 2-3-1 is a kind of titanium alloy or lucite, and foaming chamber 2-3-2 and blood storage chamber 2-3-4 shell are made by lucite or glass, and froth breaking and the 2-3-3 of filter chamber are made of foam sponge that has defoamer and filter screen.

1. a stripped pulmonary circulation perfusion new equipment is made up of respiratory ventilation system, blood circulation and constant temperature system three parts; The respiratory ventilation system is made up of respirator (1-1), airway (1-2) and pressure monitoring mouth (1-3); Blood circulation is made up of stripped lung (2-1), blood storage groove (2-2), blood pump (2-4), connection emissary vein (2-5), blood sampling mouth (2-6); Constant temperature system is made up of constant temperature lung box (3-1), thermostat (3-2), water pump (3-3), heat exchanger (3-4) and water circulating pipe (3-5); It is characterized in that: deaerator (2-3) is set in blood circulation; Deaerator is made up of foaming plate (2-3-1), foaming chamber (2-3-2), froth breaking and filter chamber (2-3-3) and blood storage chamber (2-3-4); Deaerator is connected between blood storage groove (2-2) and the blood pump (2-4), and promptly the foaming chamber of deaerator (2-3-2) is communicated with blood storage groove (2-2), and its blood storage chamber (2-3-4) is communicated with blood pump (2-4).