pmc.ncbi.nlm.nih.gov

Interactions between potassium ions and glycine transport in the yeast Saccharomyces carlsbergensis

Abstract

A study has been made of the effects of both varying the pH and extracellular [K+] on the initial rate of uptake of glycine (v) by a strain of Saccharomyces carlsbergensis that concentrated the amino acid, with respect to the extracellular phase, by up to 1400 times. When no other substrate than glycine was provided and [glycine] was relatively small (≤0.2mm) (1) v increased fivefold when the pH was lowered from 7 to 4.5; (2) v fell by up to about 80% as [K+] rose, K+ behaving as a non-competitive inhibitor of the system, with Ki 0.33mequiv./l at pH7; (3) the absorption of glycine caused up to about 2 or 3 equiv. of K+ to leave the yeast cells. These three phenomena were each less evident when glucose was present. An analogy is drawn between the respective interactions of H+ and K+ with the yeast system and the well recognized effects of Na+ and K+ on amino acid transport in certain mammalian systems.

845

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CONWAY E. J., DUGGAN F. A cation carrier in the yeast cell wall. Biochem J. 1958 Jun;69(2):265–274. doi: 10.1042/bj0690265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Curran P. F., Schultz S. G., Chez R. A., Fuisz R. E. Kinetic relations of the Na-amino acid interaction at the mucosal border of intestine. J Gen Physiol. 1967 May;50(5):1261–1286. doi: 10.1085/jgp.50.5.1261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DAVIES R., FOLKES J. P., GALE E. F., BIGGER L. C. The assimilation of amino-acids by micro-organisms. XVI. Changes in sodium and potassium accompanying the accumulation of glutamic acid or lysine by bacteria and yeast. Biochem J. 1953 Jun;54(3):430–437. doi: 10.1042/bj0540430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EDDY A. A., RUDIN A. D. The structure of the yeast cell wall. I. Identification of charged groups at the surface. Proc R Soc Lond B Biol Sci. 1958 Mar 18;148(932):419–432. doi: 10.1098/rspb.1958.0035. [DOI] [PubMed] [Google Scholar]
  5. Eddy A. A. A net gain of sodium ions and a net loss of potassium ions accompanying the uptake of glycine by mouse ascites-tumour cells in the presence of sodium cyanide. Biochem J. 1968 Jun;108(2):195–206. doi: 10.1042/bj1080195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eddy A. A. A sodium ion concentration gradient formed during the absorption of glycine by mouse ascites-tumour cells. Biochem J. 1969 Nov;115(3):505–509. doi: 10.1042/bj1150505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eddy A. A., Backen K., Nowacki J. Translocation of protons and alkali-metal cations accompanying the uptake of neutral amino acids by yeast. Biochem J. 1970 Feb;116(4):34P–35P. doi: 10.1042/bj1160034pb. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eddy A. A., Hogg M. C. Further observations on the inhibitory effect of extracellular potassium ions on glycine uptake by mouse ascites-tumour cells. Biochem J. 1969 Oct;114(4):807–814. doi: 10.1042/bj1140807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eddy A. A., Mulcahy M. F., Thomson P. J. The effects of sodium ions and potassium ions on glycine uptake by mouse ascites-tumour cells in the presence and absence of selected metabolic inhibitors. Biochem J. 1967 Jun;103(3):863–876. doi: 10.1042/bj1030863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Eddy A. A. The effects of varying the cellular and extracellular concentrations of sodium and potassium ions on the uptake of glycine by mouse ascites-tumour cells in the presence and absence of sodium cyanide. Biochem J. 1968 Jul;108(3):489–498. doi: 10.1042/bj1080489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GALE E. F. Assimilation of amino acids by Gram-positive bacteria and some actions of antibiotics thereon. Adv Protein Chem. 1953;8:285–391. doi: 10.1016/s0065-3233(08)60094-7. [DOI] [PubMed] [Google Scholar]
  12. GALE E. F., FOLKES J. P. The assimilation of amino acids by bacteria. 18. The incorporation of glutamic acid into the protein fraction of Staphylococcus aureus. Biochem J. 1953 Dec;55(5):721–729. doi: 10.1042/bj0550721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gale E. F., Folkes J. P. The effect of lipids on the accumulation of certain amino acids by Staphylococcus aureus. Biochim Biophys Acta. 1967 Oct 2;144(2):461–466. doi: 10.1016/0005-2760(67)90177-4. [DOI] [PubMed] [Google Scholar]
  14. Gits J. J., Grenson M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. 3. Evidence for a specific methionine-transporting system. Biochim Biophys Acta. 1967 Jul 3;135(3):507–516. doi: 10.1016/0005-2736(67)90040-5. [DOI] [PubMed] [Google Scholar]
  15. Grenson M., Crabeel M., Wiame J. M., Béchet J. Inhibition of protein synthesis and simulation of permease turnover in yeast. Biochem Biophys Res Commun. 1968 Feb 26;30(4):414–419. doi: 10.1016/0006-291x(68)90760-2. [DOI] [PubMed] [Google Scholar]
  16. Harold F. M., Baarda J. R. Effects of nigericin and monactin on cation permeability of Streptococcus faecalis and metabolic capacities of potassium-depleted cells. J Bacteriol. 1968 Mar;95(3):816–823. doi: 10.1128/jb.95.3.816-823.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Harold F. M., Baarda J. R. Inhibition of membrane transport in Streptococcus faecalis by uncouplers of oxidative phosphorylation and its relationship to proton conduction. J Bacteriol. 1968 Dec;96(6):2025–2034. doi: 10.1128/jb.96.6.2025-2034.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. KEMPNER E. S., COWIE D. B. Metabolic pools and the utilization of amino acid analogs for protein synthesis. Biochim Biophys Acta. 1960 Aug 26;42:401–408. doi: 10.1016/0006-3002(60)90817-9. [DOI] [PubMed] [Google Scholar]
  19. Magaña-Schwencke N., Schwencke J. A proline transport system in Saccharomyces chevalieri. Biochim Biophys Acta. 1969 Mar 11;173(2):313–323. doi: 10.1016/0005-2736(69)90114-x. [DOI] [PubMed] [Google Scholar]
  20. SKOU J. C. ENZYMATIC BASIS FOR ACTIVE TRANSPORT OF NA+ AND K+ ACROSS CELL MEMBRANE. Physiol Rev. 1965 Jul;45:596–617. doi: 10.1152/physrev.1965.45.3.596. [DOI] [PubMed] [Google Scholar]
  21. STACHIEWICZ E., QUASTEL J. H. Amino acid transport in yeast and effects of nystatin. Can J Biochem Physiol. 1963 Feb;41:397–407. [PubMed] [Google Scholar]
  22. Schwencke J., Magaña-Schwencke N. Derepression of a proline transport system in Saccharomyces chevalieri by nitrogen starvation. Biochim Biophys Acta. 1969 Mar 11;173(2):302–312. doi: 10.1016/0005-2736(69)90113-8. [DOI] [PubMed] [Google Scholar]
  23. Surdin Y., Sly W., Sire J., Bordes A. M., Robichon-Szulmajster H. Propriétés et contrôle génétique du système d'accumulation des acides aminés chez Saccharomyces cerevisiae. Biochim Biophys Acta. 1965 Oct 18;107(3):546–566. [PubMed] [Google Scholar]
  24. VIDAVER G. A. GLYCINE TRANSPORT BY HEMOLYZED AND RESTORED PIGEON RED CELLS. Biochemistry. 1964 Jun;3:795–799. doi: 10.1021/bi00894a011. [DOI] [PubMed] [Google Scholar]
  25. WILBRANDT W., ROSENBERG T. The concept of carrier transport and its corollaries in pharmacology. Pharmacol Rev. 1961 Jun;13:109–183. [PubMed] [Google Scholar]
  26. Wong P. T., Thompson J., MacLeod R. A. Nutrition and metabolism of marine bacteria. XVII. Ion-dependent retention of alpha-aminoisobutyric acid and its relation to Na+ dependent transport in a marine pseudomonad. J Biol Chem. 1969 Feb 10;244(3):1016–1025. [PubMed] [Google Scholar]