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Immunohistological distribution of 5T4 antigen in normal and malignant tissues

Abstract

A trophoblast cell surface antigen has been characterised by a monoclonal antibody (mAb) 5T4, raised following immunisation with solubilised wheat germ agglutinin binding glycoproteins from human syncytiotrophoblast plasma membrane (StMPM). The expression of the 72 kDa glycoprotein was assessed on cryostat sections of a range of neoplastic and non-neoplastic tissues, using an avidin-biotin immunoperoxidase technique. In products of conception, intense reactions were noted with villous syncytiotrophoblast membrane in normal early and term placenta, with weaker positivity of placental site trophoblast. Most normal or non-neoplastic tissues were negative, including liver, kidney, spleen, small intestine, ovary and testis. Faint or moderate positive reactions were present in some specialised epithelia. Of 115 neoplasms examined, 76 showed reactions with tumour cells including carcinomas of the bladder, breast, cervix, endometrium, lung, oesophagus, ovary, pancreas, stomach and testicular non-seminomatous germ cell tumours. Choriocarcinomas and placental site trophoblastic tumours were also positive. Most adenocarcinomas of colon and seminomas were negative as were all malignant melanomas and malignant lymphomas. A radioimmunoassay did not detect the antigen in either normal or pregnancy serum. The relatively low level of expression in normal tissues and reactivity with a wide range of carcinomas suggested that the antibody may be useful in diagnostic or targeting studies.

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Selected References

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  1. Epenetos A. A., Snook D., Hooker G., Begent R., Durbin H., Oliver R. T., Bodmer W. F., Lavender J. P. Indium-111 labelled monoclonal antibody to placental alkaline phosphatase in in the detection of neoplasms of testis, ovary, and cervix. Lancet. 1985 Aug 17;2(8451):350–353. doi: 10.1016/s0140-6736(85)92495-x. [DOI] [PubMed] [Google Scholar]
  2. Epenetos A. A., Travers P., Gatter K. C., Oliver R. D., Mason D. Y., Bodmer W. F. An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase. Br J Cancer. 1984 Jan;49(1):11–15. doi: 10.1038/bjc.1984.3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hole N., Stern P. L. A 72 kD trophoblast glycoprotein defined by a monoclonal antibody. Br J Cancer. 1988 Mar;57(3):239–246. doi: 10.1038/bjc.1988.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kardana A., Taylor M. E., Southall P. J., Boxer G. M., Rowan A. J., Bagshawe K. D. Urinary gonadotrophin peptide--isolation and purification, and its immunohistochemical distribution in normal and neoplastic tissues. Br J Cancer. 1988 Sep;58(3):281–286. doi: 10.1038/bjc.1988.204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Loke Y. W., Butterworth B. H., Margetts J. J., Burland K. Identification of cytotrophoblast colonies in cultures of human placental cells using monoclonal antibodies. Placenta. 1986 May-Jun;7(3):221–231. doi: 10.1016/s0143-4004(86)80160-6. [DOI] [PubMed] [Google Scholar]
  6. Martin K. W., Halpern S. E. Carcinoembryonic antigen production, secretion, and kinetics in BALB/c mice and a nude mouse-human tumor model. Cancer Res. 1984 Dec;44(12 Pt 1):5475–5481. [PubMed] [Google Scholar]
  7. McLaughlin P. J., Cheng M. H., Slade M. B., Johnson P. M. Expression on cultured human tumour cells of placental trophoblast membrane antigens and placental alkaline phosphatase defined by monoclonal antibodies. Int J Cancer. 1982 Jul 15;30(1):21–26. doi: 10.1002/ijc.2910300105. [DOI] [PubMed] [Google Scholar]
  8. McLaughlin P. J., Gee H., Johnson P. M. Placental-type alkaline phosphatase in pregnancy and malignancy plasma: specific estimation using a monoclonal antibody in a solid phase enzyme immunoassay. Clin Chim Acta. 1983 May 30;130(2):199–209. doi: 10.1016/0009-8981(83)90117-1. [DOI] [PubMed] [Google Scholar]
  9. Stern P. L., Beresford N., Thompson S., Johnson P. M., Webb P. D., Hole N. Characterization of the human trophoblast-leukocyte antigenic molecules defined by a monoclonal antibody. J Immunol. 1986 Sep 1;137(5):1604–1609. [PubMed] [Google Scholar]
  10. Welte K., Platzer E., Lu L., Gabrilove J. L., Levi E., Mertelsmann R., Moore M. A. Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1526–1530. doi: 10.1073/pnas.82.5.1526. [DOI] [PMC free article] [PubMed] [Google Scholar]