PROSITE

Description

Granulocyte-macrophage colony-stimulating factor (GM-CSF) [1,2] is a cytokine that acts in hematopoiesis by stimulating the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

GM-CSF is a glycoprotein of about 120 amino acid residues that contains four conserved cysteine residues involved in two disulfide bonds, as shown in the following schematic representation.

                                                   +---------------+
                                                   |               |
        xxxxxxxxxxxxxxxxxxxxxxxxxxCxxxxxxxxxxxxxxxxCxxxCxxxxxxxxxxxCxxx
                                  |                *****
                                  +--------------------+

'C': conserved cysteine involved in a disulfide bond.
'*': position of the pattern.

As a signature pattern, we selected the region that includes the second and third conserved cysteines.

Last update:

May 2004 / Text revised.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

GM_CSF, PS00702; Granulocyte-macrophage colony-stimulating factor signature  (PATTERN)

• Consensus pattern:
  C-P-[LP]-T-x-E-[ST]-x-C
  The 2 C's are involved in disulfide bonds
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 11
  • detected by PS00702: 11 (true positives)
  • undetected by PS00702: 0 (false negative or 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS00702:
  NONE.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00702
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00702
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS00702
• Matching PDB structures: 1CSG 2GMF 4NKQ 4RS1 ... [ALL]

References

1	Authors	Morstyn G. Burgess A.W.
Title	Hemopoietic growth factors: a review.
Source	Cancer Res. 48:5624-5637(1988).
PubMed ID	2458827

2	Authors	Walter M.R. Cook W.J. Ealick S.E. Nagabhushan T.L. Trotta P.P. Bugg C.E.
Title	Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor.
Source	J. Mol. Biol. 224:1075-1085(1992).
PubMed ID	1569568

Copyright

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