Cellular and subcellular expression of monocarboxylate transporters in the pigment epithelium and retina of the rat - PubMed

Cellular and subcellular expression of monocarboxylate transporters in the pigment epithelium and retina of the rat

L Bergersen et al. Neuroscience. 1999 Apr.

Abstract

The cellular and subcellular expression of the monocarboxylate transporters MCT1, MCT2 and MCT4 [corresponding to MCT3 of Price N. T. et al. (1998) Biochem. J. 329, 321-328] were investigated in the pigment epithelium and outer retina of rats. Immunofluorescence and postembedding immunogold analyses revealed strong MCT1 labelling in the apical membrane of the pigment epithelial and no detectable signal in the basolateral membrane. In contrast, antibodies to the glucose transporter GLUT1 produced intense labelling in both membranes. Neither MCT1 nor GLUT1 was enriched in intracellular compartments. The monocarboxylate transporter MCT4 was very weakly expressed in the retinal pigment epithelium of adult animals, but occurred at higher concentrations at this site in 14-day-old rats. However, even at the latter stage, the immunolabelling of MCT4 was weak compared to that of MCT1. In the neural retina, the data were consistent with a predominant glial localization of MCT1. Specifically, immunogold particles signalling MCT1 occurred in Müller cell microvilli and in the velate processes between the photoreceptors. No labelling was obtained with antibodies to MCT2. Taken together with previous biochemical analyses, the present findings indicate that MCT1 is involved in the outward transport of lactate through the retinal pigment epithelial cells, and in the transfer of lactate between Müller cells and photoreceptors.

Similar articles

• Polarized expression of monocarboxylate transporters in human retinal pigment epithelium and ARPE-19 cells.

  Philp NJ, Wang D, Yoon H, Hjelmeland LM. Philp NJ, et al. Invest Ophthalmol Vis Sci. 2003 Apr;44(4):1716-21. doi: 10.1167/iovs.02-0287. Invest Ophthalmol Vis Sci. 2003. PMID: 12657613

• Distribution of monocarboxylate transporters MCT1 and MCT2 in rat retina.

  Gerhart DZ, Leino RL, Drewes LR. Gerhart DZ, et al. Neuroscience. 1999;92(1):367-75. doi: 10.1016/s0306-4522(98)00699-x. Neuroscience. 1999. PMID: 10392858

• Loss of MCT1, MCT3, and MCT4 expression in the retinal pigment epithelium and neural retina of the 5A11/basigin-null mouse.

  Philp NJ, Ochrietor JD, Rudoy C, Muramatsu T, Linser PJ. Philp NJ, et al. Invest Ophthalmol Vis Sci. 2003 Mar;44(3):1305-11. doi: 10.1167/iovs.02-0552. Invest Ophthalmol Vis Sci. 2003. PMID: 12601063

• Immunogold cytochemistry identifies specialized membrane domains for monocarboxylate transport in the central nervous system.

  Bergersen L, Rafiki A, Ottersen OP. Bergersen L, et al. Neurochem Res. 2002 Feb;27(1-2):89-96. doi: 10.1023/a:1014806723147. Neurochem Res. 2002. PMID: 11926280 Review.

• The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation.

  Halestrap AP, Price NT. Halestrap AP, et al. Biochem J. 1999 Oct 15;343 Pt 2(Pt 2):281-99. Biochem J. 1999. PMID: 10510291 Free PMC article. Review.

Cited by

• Inflammatory Regulation of CNS Barriers After Traumatic Brain Injury: A Tale Directed by Interleukin-1.

  Bodnar CN, Watson JB, Higgins EK, Quan N, Bachstetter AD. Bodnar CN, et al. Front Immunol. 2021 May 21;12:688254. doi: 10.3389/fimmu.2021.688254. eCollection 2021. Front Immunol. 2021. PMID: 34093593 Free PMC article. Review.

• Enhanced expression of three monocarboxylate transporter isoforms in the brain of obese mice.

  Pierre K, Parent A, Jayet PY, Halestrap AP, Scherrer U, Pellerin L. Pierre K, et al. J Physiol. 2007 Sep 1;583(Pt 2):469-86. doi: 10.1113/jphysiol.2007.138594. Epub 2007 Jun 28. J Physiol. 2007. PMID: 17599960 Free PMC article.

• Monocarboxylate transporter 1 is deficient on microvessels in the human epileptogenic hippocampus.

  Lauritzen F, de Lanerolle NC, Lee TS, Spencer DD, Kim JH, Bergersen LH, Eid T. Lauritzen F, et al. Neurobiol Dis. 2011 Feb;41(2):577-84. doi: 10.1016/j.nbd.2010.11.005. Epub 2010 Nov 25. Neurobiol Dis. 2011. PMID: 21081165 Free PMC article.

• Essential polyunsaturated fatty acids and the barrier to the brain: the components of a model for transport.

  Edmond J. Edmond J. J Mol Neurosci. 2001 Apr-Jun;16(2-3):181-93; discussion 215-21. doi: 10.1385/JMN:16:2-3:181. J Mol Neurosci. 2001. PMID: 11478373 Review.

• Transcriptome analysis of the planarian eye identifies ovo as a specific regulator of eye regeneration.

  Lapan SW, Reddien PW. Lapan SW, et al. Cell Rep. 2012 Aug 30;2(2):294-307. doi: 10.1016/j.celrep.2012.06.018. Epub 2012 Aug 2. Cell Rep. 2012. PMID: 22884275 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Miscellaneous

  • NCI CPTAC Assay Portal