Identification of NSF as a beta-arrestin1-binding protein. Implications for beta2-adrenergic receptor regulation - PubMed
- ️Fri Jan 01 1999
. 1999 Apr 16;274(16):10677-80.
doi: 10.1074/jbc.274.16.10677.
Affiliations
- PMID: 10196135
- DOI: 10.1074/jbc.274.16.10677
Free article
Identification of NSF as a beta-arrestin1-binding protein. Implications for beta2-adrenergic receptor regulation
P H McDonald et al. J Biol Chem. 1999.
Free article
Abstract
Previous studies have demonstrated that beta-arrestin1 serves to target G protein-coupled receptors for internalization via clathrin-coated pits and that its endocytic function is regulated by dephosphorylation at the plasma membrane. Using the yeast two-hybrid system, we have identified a novel beta-arrestin1-binding protein, NSF (N-ethylmaleimide-sensitive fusion protein), an ATPase essential for many intracellular transport reactions. We demonstrate that purified recombinant beta-arrestin1 and NSF interact in vitro and that these proteins can be coimmunoprecipitated from cells. beta-Arrestin1-NSF complex formation exhibits a conformational dependence with beta-arrestin1 preferentially interacting with the ATP bound form of NSF. In contrast to the beta-arrestin1-clathrin interaction, however, the phosphorylation state of beta-arrestin1 does not affect NSF binding. Functionally, overexpression of NSF in HEK 293 cells significantly enhances agonist-mediated beta2-adrenergic receptor (beta2-AR) internalization. Furthermore, when coexpressed with a beta-arrestin1 mutant (betaarr1S412D) that mimics a constitutively phosphorylated form of beta-arrestin1 and that acts as a dominant negative with regards to beta2-AR internalization, NSF rescues the betaarr1S412D-mediated inhibition of beta2-AR internalization. The demonstration of beta-arrestin1-NSF complex formation and the functional consequences of NSF overexpression suggest a hitherto unappreciated role for NSF in facilitating clathrin coat-mediated G protein-coupled receptor internalization.
Similar articles
-
Lin FT, Krueger KM, Kendall HE, Daaka Y, Fredericks ZL, Pitcher JA, Lefkowitz RJ. Lin FT, et al. J Biol Chem. 1997 Dec 5;272(49):31051-7. doi: 10.1074/jbc.272.49.31051. J Biol Chem. 1997. PMID: 9388255
-
N-terminal tyrosine modulation of the endocytic adaptor function of the beta-arrestins.
Marion S, Fralish GB, Laporte S, Caron MG, Barak LS. Marion S, et al. J Biol Chem. 2007 Jun 29;282(26):18937-44. doi: 10.1074/jbc.M700090200. Epub 2007 Apr 24. J Biol Chem. 2007. PMID: 17456469
-
Anborgh PH, Seachrist JL, Dale LB, Ferguson SS. Anborgh PH, et al. Mol Endocrinol. 2000 Dec;14(12):2040-53. doi: 10.1210/mend.14.12.0565. Mol Endocrinol. 2000. PMID: 11117533
-
Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor.
Whiteheart SW, Matveeva EA. Whiteheart SW, et al. J Struct Biol. 2004 Apr-May;146(1-2):32-43. doi: 10.1016/j.jsb.2003.09.015. J Struct Biol. 2004. PMID: 15037235 Review.
Cited by
-
Ubiquitin-mediated regulation of endocytosis by proteins of the arrestin family.
Becuwe M, Herrador A, Haguenauer-Tsapis R, Vincent O, Léon S. Becuwe M, et al. Biochem Res Int. 2012;2012:242764. doi: 10.1155/2012/242764. Epub 2012 Sep 4. Biochem Res Int. 2012. PMID: 22988512 Free PMC article.
-
A genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth.
Laviolette MJ, Nunes P, Peyre JB, Aigaki T, Stewart BA. Laviolette MJ, et al. Genetics. 2005 Jun;170(2):779-92. doi: 10.1534/genetics.104.035691. Epub 2005 Apr 16. Genetics. 2005. PMID: 15834148 Free PMC article.
-
Kook S, Gurevich VV, Gurevich EV. Kook S, et al. Handb Exp Pharmacol. 2014;219:309-39. doi: 10.1007/978-3-642-41199-1_16. Handb Exp Pharmacol. 2014. PMID: 24292837 Free PMC article.
-
Multifaceted role of β-arrestins in inflammation and disease.
Sharma D, Parameswaran N. Sharma D, et al. Genes Immun. 2015 Dec;16(8):499-513. doi: 10.1038/gene.2015.37. Epub 2015 Sep 17. Genes Immun. 2015. PMID: 26378652 Free PMC article. Review.
-
Defective lymphocyte chemotaxis in beta-arrestin2- and GRK6-deficient mice.
Fong AM, Premont RT, Richardson RM, Yu YR, Lefkowitz RJ, Patel DD. Fong AM, et al. Proc Natl Acad Sci U S A. 2002 May 28;99(11):7478-83. doi: 10.1073/pnas.112198299. Proc Natl Acad Sci U S A. 2002. PMID: 12032308 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous