Action of DNA repair endonuclease ERCC1/XPF in living cells - PubMed

Action of DNA repair endonuclease ERCC1/XPF in living cells

A B Houtsmuller et al. Science. 1999.

Abstract

To study the nuclear organization and dynamics of nucleotide excision repair (NER), the endonuclease ERCC1/XPF (for excision repair cross complementation group 1/xeroderma pigmentosum group F) was tagged with green fluorescent protein and its mobility was monitored in living Chinese hamster ovary cells. In the absence of DNA damage, the complex moved freely through the nucleus, with a diffusion coefficient (15 +/- 5 square micrometers per second) consistent with its molecular size. Ultraviolet light-induced DNA damage caused a transient dose-dependent immobilization of ERCC1/XPF, likely due to engagement of the complex in a single repair event. After 4 minutes, the complex regained mobility. These results suggest (i) that NER operates by assembly of individual NER factors at sites of DNA damage rather than by preassembly of holocomplexes and (ii) that ERCC1/XPF participates in repair of DNA damage in a distributive fashion rather than by processive scanning of large genome segments.

Similar articles

• Mislocalization of XPF-ERCC1 nuclease contributes to reduced DNA repair in XP-F patients.

  Ahmad A, Enzlin JH, Bhagwat NR, Wijgers N, Raams A, Appledoorn E, Theil AF, J Hoeijmakers JH, Vermeulen W, J Jaspers NG, Schärer OD, Niedernhofer LJ. Ahmad A, et al. PLoS Genet. 2010 Mar 5;6(3):e1000871. doi: 10.1371/journal.pgen.1000871. PLoS Genet. 2010. PMID: 20221251 Free PMC article.

• The formation of UV-induced chromosome aberrations involves ERCC1 and XPF but not other nucleotide excision repair genes.

  Chipchase MD, Melton DW. Chipchase MD, et al. DNA Repair (Amst). 2002 Apr 29;1(4):335-40. doi: 10.1016/s1568-7864(02)00010-1. DNA Repair (Amst). 2002. PMID: 12509251

• Recruitment of the nucleotide excision repair endonuclease XPG to sites of UV-induced dna damage depends on functional TFIIH.

  Zotter A, Luijsterburg MS, Warmerdam DO, Ibrahim S, Nigg A, van Cappellen WA, Hoeijmakers JH, van Driel R, Vermeulen W, Houtsmuller AB. Zotter A, et al. Mol Cell Biol. 2006 Dec;26(23):8868-79. doi: 10.1128/MCB.00695-06. Epub 2006 Sep 25. Mol Cell Biol. 2006. PMID: 17000769 Free PMC article.

• Function and Interactions of ERCC1-XPF in DNA Damage Response.

  Faridounnia M, Folkers GE, Boelens R. Faridounnia M, et al. Molecules. 2018 Dec 5;23(12):3205. doi: 10.3390/molecules23123205. Molecules. 2018. PMID: 30563071 Free PMC article. Review.

• Interstrand crosslink repair: can XPF-ERCC1 be let off the hook?

  Bergstralh DT, Sekelsky J. Bergstralh DT, et al. Trends Genet. 2008 Feb;24(2):70-6. doi: 10.1016/j.tig.2007.11.003. Epub 2008 Jan 14. Trends Genet. 2008. PMID: 18192062 Review.

Cited by

• Attenuated NER expressions of XPF and XPC associated with smoking are involved in the recurrence of bladder cancer.

  Qiu J, Wang X, Meng X, Zheng Y, Li G, Ma J, Ye G, Li Y, Li J. Qiu J, et al. PLoS One. 2014 Dec 23;9(12):e115224. doi: 10.1371/journal.pone.0115224. eCollection 2014. PLoS One. 2014. PMID: 25535740 Free PMC article.

• Single Cell Quantification of Reporter Gene Expression in Live Adult Caenorhabditis elegans Reveals Reproducible Cell-Specific Expression Patterns and Underlying Biological Variation.

  Mendenhall AR, Tedesco PM, Sands B, Johnson TE, Brent R. Mendenhall AR, et al. PLoS One. 2015 May 6;10(5):e0124289. doi: 10.1371/journal.pone.0124289. eCollection 2015. PLoS One. 2015. PMID: 25946008 Free PMC article.

• Cdt1 associates dynamically with chromatin throughout G1 and recruits Geminin onto chromatin.

  Xouri G, Squire A, Dimaki M, Geverts B, Verveer PJ, Taraviras S, Nishitani H, Houtsmuller AB, Bastiaens PI, Lygerou Z. Xouri G, et al. EMBO J. 2007 Mar 7;26(5):1303-14. doi: 10.1038/sj.emboj.7601597. Epub 2007 Feb 22. EMBO J. 2007. PMID: 17318181 Free PMC article.

• Nuclear dynamics of PCNA in DNA replication and repair.

  Essers J, Theil AF, Baldeyron C, van Cappellen WA, Houtsmuller AB, Kanaar R, Vermeulen W. Essers J, et al. Mol Cell Biol. 2005 Nov;25(21):9350-9. doi: 10.1128/MCB.25.21.9350-9359.2005. Mol Cell Biol. 2005. PMID: 16227586 Free PMC article.

• Domain swapping between FEN-1 and XPG defines regions in XPG that mediate nucleotide excision repair activity and substrate specificity.

  Hohl M, Dunand-Sauthier I, Staresincic L, Jaquier-Gubler P, Thorel F, Modesti M, Clarkson SG, Schärer OD. Hohl M, et al. Nucleic Acids Res. 2007;35(9):3053-63. doi: 10.1093/nar/gkm092. Epub 2007 Apr 22. Nucleic Acids Res. 2007. PMID: 17452369 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Molecular Biology Databases

  • BioCyc