Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool - PubMed

Affiliations

• PMID: 10357847

Free article

Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool

A K Batta et al. J Lipid Res. 1999 Jun.

Free article

Abstract

A simple method for the gas-liquid chromatographic quantitation of human fecal bile acids and sterols is described where bile acids are subjected to n-butyl ester derivatization, without prior isolation from the stool, followed by trimethylsilylation of the sterols and bile acids. Under these conditions, bile acid derivatives are well resolved from each other and from the trimethylsilyl ether derivatives of fecal sterols and no overlap occurs. The method was shown to be highly reproducible and recoveries were similar to those obtained with other methods used for fecal bile acid analysis. Application of the method for bile acid and sterol analysis in human stool is described.

Similar articles

• Simultaneous quantitation of fatty acids, sterols and bile acids in human stool by capillary gas-liquid chromatography.

  Batta AK, Salen G, Batta P, Tint GS, Alberts DS, Earnest DL. Batta AK, et al. J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Aug 5;775(2):153-61. doi: 10.1016/s1570-0232(02)00289-1. J Chromatogr B Analyt Technol Biomed Life Sci. 2002. PMID: 12113981

• Fecal steroids in polyposis coli and ileorectostomy patients.

  Watne AL, Core SK. Watne AL, et al. J Surg Res. 1975 Sep;19(3):157-61. doi: 10.1016/0022-4804(75)90075-x. J Surg Res. 1975. PMID: 1100901 No abstract available.

• Faecal pH, bile acid and sterol concentrations in premenopausal Indian and white vegetarians compared with white omnivores.

  Reddy S, Sanders TA, Owen RW, Thompson MH. Reddy S, et al. Br J Nutr. 1998 Jun;79(6):495-500. doi: 10.1079/bjn19980087. Br J Nutr. 1998. PMID: 9771336

• Microbiological aspects of enterohepatic neutral sterol and bile acid metabolism.

  Kellogg TF. Kellogg TF. Fed Proc. 1971 Nov-Dec;30(6):1808-14. Fed Proc. 1971. PMID: 4942052 Review. No abstract available.

• Analysis of sterols: application to sterol balance studies.

  Subbiah MT. Subbiah MT. Am J Clin Nutr. 1972 Aug;25(8):780-8. doi: 10.1093/ajcn/25.8.780. Am J Clin Nutr. 1972. PMID: 4558369 Review. No abstract available.

Cited by

• The role of bile Acid excretion in atherosclerotic coronary artery disease.

  Charach G, Rabinovich A, Argov O, Weintraub M, Rabinovich P. Charach G, et al. Int J Vasc Med. 2012;2012:949672. doi: 10.1155/2012/949672. Epub 2011 Sep 12. Int J Vasc Med. 2012. PMID: 21918722 Free PMC article.

• Muricholic Acids Promote Resistance to Hypercholesterolemia in Cholesterol-Fed Mice.

  Gaillard D, Masson D, Garo E, Souidi M, Pais de Barros JP, Schoonjans K, Grober J, Besnard P, Thomas C. Gaillard D, et al. Int J Mol Sci. 2021 Jul 2;22(13):7163. doi: 10.3390/ijms22137163. Int J Mol Sci. 2021. PMID: 34281217 Free PMC article.

• Determination of Fecal Sterols Following a Diet with and without Plant Sterols.

  Cuevas-Tena M, Alegría A, Lagarda MJ. Cuevas-Tena M, et al. Lipids. 2017 Oct;52(10):871-884. doi: 10.1007/s11745-017-4286-6. Epub 2017 Sep 6. Lipids. 2017. PMID: 28879642

• Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals Biomarkers Relevant to Personalized Treatments.

  Guo N, Wang P, Yang J, Yang X, van der Voet M, Wildwater M, Wei J, Tang X, Wang M, Yang H. Guo N, et al. Front Pharmacol. 2021 Jun 14;12:664320. doi: 10.3389/fphar.2021.664320. eCollection 2021. Front Pharmacol. 2021. PMID: 34194326 Free PMC article.

• Procollagen C-endopeptidase Enhancer Protein 2 (PCPE2) Reduces Atherosclerosis in Mice by Enhancing Scavenger Receptor Class B1 (SR-BI)-mediated High-density Lipoprotein (HDL)-Cholesteryl Ester Uptake.

  Pollard RD, Blesso CN, Zabalawi M, Fulp B, Gerelus M, Zhu X, Lyons EW, Nuradin N, Francone OL, Li XA, Sahoo D, Thomas MJ, Sorci-Thomas MG. Pollard RD, et al. J Biol Chem. 2015 Jun 19;290(25):15496-15511. doi: 10.1074/jbc.M115.646240. Epub 2015 May 6. J Biol Chem. 2015. PMID: 25947382 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Medical

  • MedlinePlus Health Information