Aspirin induces cell death and caspase-dependent phosphatidylserine externalization in HT-29 human colon adenocarcinoma cells - PubMed

Aspirin induces cell death and caspase-dependent phosphatidylserine externalization in HT-29 human colon adenocarcinoma cells

E Castaño et al. Br J Cancer. 1999 Sep.

Free PMC article

Abstract

The induction of cell death by aspirin was analysed in HT-29 colon carcinoma cells. Aspirin induced two hallmarks of apoptosis: nuclear chromatin condensation and increase in phosphatidylserine externalization. However, aspirin did not induce either oligonucleosomal fragmentation of DNA, decrease in DNA content or nuclear fragmentation. The effect of aspirin on Annexin V binding was inhibited by the caspase inhibitor Z-VAD.fmk, indicating the involvement of caspases in the apoptotic action of aspirin. However, aspirin did not induce proteolysis of PARP, suggesting that aspirin does not increase nuclear caspase 3-like activity in HT-29 cells. This finding may be related with the 'atypical' features of aspirin-induced apoptosis in HT-29 cells.

Similar articles

• Tumor necrosis factor-alpha induces apoptosis associated with poly(ADP-ribose) polymerase cleavage in HT-29 colon cancer cells.

  Vaculová A, Hofmanova J, Soucek K, Kovariková M, Kozubík A. Vaculová A, et al. Anticancer Res. 2002 May-Jun;22(3):1635-9. Anticancer Res. 2002. PMID: 12168847

• Synthetic 1,4-anthracenedione analogs induce cytochrome c release, caspase-9, -3, and -8 activities, poly(ADP-ribose) polymerase-1 cleavage and internucleosomal DNA fragmentation in HL-60 cells by a mechanism which involves caspase-2 activation but not Fas signaling.

  Perchellet EM, Wang Y, Weber RL, Sperfslage BJ, Lou K, Crossland J, Hua DH, Perchellet JP. Perchellet EM, et al. Biochem Pharmacol. 2004 Feb 1;67(3):523-37. doi: 10.1016/j.bcp.2003.09.012. Biochem Pharmacol. 2004. PMID: 15037204

• Mechanism of ricin-induced apoptosis in human cervical cancer cells.

  Rao PV, Jayaraj R, Bhaskar AS, Kumar O, Bhattacharya R, Saxena P, Dash PK, Vijayaraghavan R. Rao PV, et al. Biochem Pharmacol. 2005 Mar 1;69(5):855-65. doi: 10.1016/j.bcp.2004.11.010. Epub 2005 Jan 22. Biochem Pharmacol. 2005. PMID: 15710362

• Apoptotic Features in Non-Apoptotic Processes.

  Savitskaya MA, Zakharov II, Onishchenko GE. Savitskaya MA, et al. Biochemistry (Mosc). 2022 Mar;87(3):191-206. doi: 10.1134/S0006297922030014. Biochemistry (Mosc). 2022. PMID: 35526851 Review.

• [Biochemical changes in apoptosis and methods for their determination (review)].

  Sedláková A, Kohút A, Kalina I. Sedláková A, et al. Cesk Fysiol. 1999 Aug;48(3):107-18. Cesk Fysiol. 1999. PMID: 10568073 Review. Slovak.

Cited by

• Aspirin-induced mucosal cell death in human gastric cells: evidence supporting an apoptotic mechanism.

  Power JJ, Dennis MS, Redlak MJ, Miller TA. Power JJ, et al. Dig Dis Sci. 2004 Sep;49(9):1518-25. doi: 10.1023/b:ddas.0000042258.41480.30. Dig Dis Sci. 2004. PMID: 15481331

• The historical analysis of aspirin discovery, its relation to the willow tree and antiproliferative and anticancer potential.

  Mahdi JG, Mahdi AJ, Mahdi AJ, Bowen ID. Mahdi JG, et al. Cell Prolif. 2006 Apr;39(2):147-55. doi: 10.1111/j.1365-2184.2006.00377.x. Cell Prolif. 2006. PMID: 16542349 Free PMC article. Review.

• Evidence for colorectal cancer cell specificity of aspirin effects on NF kappa B signalling and apoptosis.

  Din FV, Dunlop MG, Stark LA. Din FV, et al. Br J Cancer. 2004 Jul 19;91(2):381-8. doi: 10.1038/sj.bjc.6601913. Br J Cancer. 2004. PMID: 15188000 Free PMC article.

• Aspirin-induced apoptosis in jurkat cells is not mediated by peroxisome proliferator-activated receptor delta.

  López JM, Fernández MA, Piqué M, Gil J. López JM, et al. Mol Cell Biochem. 2004 Nov;266(1-2):57-63. doi: 10.1023/b:mcbi.0000049138.67699.7b. Mol Cell Biochem. 2004. PMID: 15646027

• The proapoptotic effect of traditional and novel nonsteroidal anti-inflammatory drugs in mammalian and yeast cells.

  Farrugia G, Balzan R. Farrugia G, et al. Oxid Med Cell Longev. 2013;2013:504230. doi: 10.1155/2013/504230. Epub 2013 Aug 1. Oxid Med Cell Longev. 2013. PMID: 23983899 Free PMC article. Review.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Diposit Digital de la Universitat de Barcelona
  • Europe PubMed Central
  • Nature Publishing Group
  • PubMed Central

• Research Materials

  • NCI CPTC Antibody Characterization Program