Rho-specific binding and guanine nucleotide exchange catalysis by KIAA0380, a dbl family member - PubMed

Rho-specific binding and guanine nucleotide exchange catalysis by KIAA0380, a dbl family member

U Rümenapp et al. FEBS Lett. 1999.

Free article

Erratum in

• FEBS Lett 2000 Feb 4;467(1):134-5

Abstract

Several guanine nucleotide exchange factors (GEFs) for Rho-GTPases have been identified, all of them containing a Dbl homology (DH) and pleckstrin homology (PH) domain, but exhibiting different specificities to the Rho family members, Rho, Rac and Cdc42. We report here that KIAA0380, a protein with a tandem DH/PH domain, an amino-terminal PDZ domain and a regulator of G protein signalling (RGS) homology domain, is a specific GEF for RhoA, but not for Rac1 and Cdc42, as determined by GDP release, guanosine 5'-O-(3-thio)triphosphate (GTPgammaS) binding and protein binding assays. When expressed in J82 cells, DH/PH domain-containing forms of KIAA0380 induced actin stress fibers, whereas expression of the RGS homology domain prevented lysophosphatidic acid (LPA)-induced stress fiber formation.

Similar articles

• Mechanistic insights into specificity, activity, and regulatory elements of the regulator of G-protein signaling (RGS)-containing Rho-specific guanine nucleotide exchange factors (GEFs) p115, PDZ-RhoGEF (PRG), and leukemia-associated RhoGEF (LARG).

  Jaiswal M, Gremer L, Dvorsky R, Haeusler LC, Cirstea IC, Uhlenbrock K, Ahmadian MR. Jaiswal M, et al. J Biol Chem. 2011 May 20;286(20):18202-12. doi: 10.1074/jbc.M111.226431. Epub 2011 Mar 28. J Biol Chem. 2011. PMID: 21454492 Free PMC article.

• Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases.

  Ren Y, Li R, Zheng Y, Busch H. Ren Y, et al. J Biol Chem. 1998 Dec 25;273(52):34954-60. doi: 10.1074/jbc.273.52.34954. J Biol Chem. 1998. PMID: 9857026

• Physical and functional interactions of the lysophosphatidic acid receptors with PDZ domain-containing Rho guanine nucleotide exchange factors (RhoGEFs).

  Yamada T, Ohoka Y, Kogo M, Inagaki S. Yamada T, et al. J Biol Chem. 2005 May 13;280(19):19358-63. doi: 10.1074/jbc.M414561200. Epub 2005 Mar 8. J Biol Chem. 2005. PMID: 15755723

• The guanine nucleotide exchange factor Tiam1: a Janus-faced molecule in cellular signaling.

  Boissier P, Huynh-Do U. Boissier P, et al. Cell Signal. 2014 Mar;26(3):483-91. doi: 10.1016/j.cellsig.2013.11.034. Epub 2013 Dec 2. Cell Signal. 2014. PMID: 24308970 Review.

• The GAPs, GEFs, and GDIs of heterotrimeric G-protein alpha subunits.

  Siderovski DP, Willard FS. Siderovski DP, et al. Int J Biol Sci. 2005;1(2):51-66. doi: 10.7150/ijbs.1.51. Epub 2005 Apr 1. Int J Biol Sci. 2005. PMID: 15951850 Free PMC article. Review.

Cited by

• RHGF-2 is an essential Rho-1 specific RhoGEF that binds to the multi-PDZ domain scaffold protein MPZ-1 in Caenorhabditis elegans.

  Lin L, Tran T, Hu S, Cramer T, Komuniecki R, Steven RM. Lin L, et al. PLoS One. 2012;7(2):e31499. doi: 10.1371/journal.pone.0031499. Epub 2012 Feb 20. PLoS One. 2012. PMID: 22363657 Free PMC article.

• p63RhoGEF and GEFT are Rho-specific guanine nucleotide exchange factors encoded by the same gene.

  Lutz S, Freichel-Blomquist A, Rümenapp U, Schmidt M, Jakobs KH, Wieland T. Lutz S, et al. Naunyn Schmiedebergs Arch Pharmacol. 2004 May;369(5):540-6. doi: 10.1007/s00210-004-0926-5. Epub 2004 Apr 7. Naunyn Schmiedebergs Arch Pharmacol. 2004. PMID: 15069594

• R1467H Variants of Rho Guanine Nucleotide Exchange Factor 11 (ARHGEF11) are Associated with Type 2 Diabetes Mellitus in Koreans.

  Jin QS, Kim SH, Piao SJ, Lim HA, Lee SY, Hong SB, Kim YS, Lee HJ, Nam M. Jin QS, et al. Korean Diabetes J. 2010 Dec;34(6):368-73. doi: 10.4093/kdj.2010.34.6.368. Epub 2010 Dec 31. Korean Diabetes J. 2010. PMID: 21246010 Free PMC article.

• Human Rho Guanine Nucleotide Exchange Factor 11 (ARHGEF11) Regulates Dendritic Morphogenesis.

  Mizuki Y, Takaki M, Sakamoto S, Okamoto S, Kishimoto M, Okahisa Y, Itoh M, Yamada N. Mizuki Y, et al. Int J Mol Sci. 2016 Dec 29;18(1):67. doi: 10.3390/ijms18010067. Int J Mol Sci. 2016. PMID: 28036092 Free PMC article.

• Signalling mechanisms of RhoGTPase regulation by the heterotrimeric G proteins G12 and G13.

  Kozasa T, Hajicek N, Chow CR, Suzuki N. Kozasa T, et al. J Biochem. 2011 Oct;150(4):357-69. doi: 10.1093/jb/mvr105. Epub 2011 Aug 26. J Biochem. 2011. PMID: 21873336 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science
  • Wiley

• Molecular Biology Databases

  • BioCyc
  • GlyGen glycoinformatics resource

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal