G1-phase and B-type cyclins exclude the DNA-replication factor Mcm4 from the nucleus - PubMed

G1-phase and B-type cyclins exclude the DNA-replication factor Mcm4 from the nucleus

K Labib et al. Nat Cell Biol. 1999 Nov.

Abstract

Cyclin-dependent kinases (CDKs) activate the firing of replication origins during the S phase of the cell cycle. They also block re-initiation of DNA replication within a single cell cycle, by preventing the assembly of prereplicative complexes at origins. We show here that, in budding yeast, CDKs exclude the essential prereplicative-complex component Mcm4 from the nucleus. Although origin firing can be triggered by the B-type cyclins only, both G1-phase and B-type cyclins cause exit of Mcm4 from the nucleus. These results suggest that G1 cyclins may diminish the cell's capacity to assemble prereplicative complexes before B-type cyclins trigger origin firing during S phase.

Comment in

• Saying a firm 'no' to DNA re-replication.

  Blow JJ, Prokhorova TA. Blow JJ, et al. Nat Cell Biol. 1999 Nov;1(7):E175-7. doi: 10.1038/15611. Nat Cell Biol. 1999. PMID: 10559996 Free PMC article.

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