pubmed.ncbi.nlm.nih.gov

Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth - PubMed

Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth

J R Kerr et al. J Clin Pathol. 1999 May.

Abstract

Aim: To examine strains of Pseudomonas aeruginosa for specific antifungal factors.

Methods: Two clinical strains of P aeruginosa with strong in vitro inhibition (by cross streak assay) of Candida albicans and Aspergillus fumigatus were examined. Both strains were isolated from sputum--one from a patient with cystic fibrosis and one from a patient with bronchiectasis. Bacterial extracts were fractionated by high performance liquid chromatography and examined by ultraviolet absorbance and mass spectroscopy. Antifungal activity against C albicans and A fumigatus was determined in a well plate assay.

Results: Pyocyanin was the major antifungal agent of P aeruginosa; 1-hydroxy-phenazine also possessed activity. Pyocyanin MICs for C albicans and A fumigatus were > 64 micrograms/ml. These phenazines were active against nine other yeast species pathogenic for man. Preliminary experiments also suggested possible inhibition of yeast mycelial transformation in C albicans by pyocyanin.

Conclusions: There may be a role for pyocyanin and 1-hydroxyphenazine in the prevention of pulmonary candidiasis in patients colonised by P aeruginosa.

PubMed Disclaimer

Cited by

Optimization of Culture Conditions for Mass Production of the Probiotics Pseudomonas MCCB 102 and 103 Antagonistic to Pathogenic Vibrios in Aquaculture.
Preetha R, Vijayan KK, Jayapraksh NS, Alavandi SV, Santiago TC, Singh IS. Preetha R, et al. Probiotics Antimicrob Proteins. 2015 Jun;7(2):137-45. doi: 10.1007/s12602-015-9185-7. Probiotics Antimicrob Proteins. 2015. PMID: 25644493
Microbial Nanoculture as an Artificial Microniche.
Niepa TH, Hou L, Jiang H, Goulian M, Koo H, Stebe KJ, Lee D. Niepa TH, et al. Sci Rep. 2016 Aug 1;6:30578. doi: 10.1038/srep30578. Sci Rep. 2016. PMID: 27476816 Free PMC article.
Inhibition of Aspergillus fumigatus and Its Biofilm by Pseudomonas aeruginosa Is Dependent on the Source, Phenotype and Growth Conditions of the Bacterium.
Ferreira JA, Penner JC, Moss RB, Haagensen JA, Clemons KV, Spormann AM, Nazik H, Cohen K, Banaei N, Carolino E, Stevens DA. Ferreira JA, et al. PLoS One. 2015 Aug 7;10(8):e0134692. doi: 10.1371/journal.pone.0134692. eCollection 2015. PLoS One. 2015. PMID: 26252384 Free PMC article.
Bacterial-fungal interactions and their impact on microbial pathogenesis.
MacAlpine J, Robbins N, Cowen LE. MacAlpine J, et al. Mol Ecol. 2023 May;32(10):2565-2581. doi: 10.1111/mec.16411. Epub 2022 Mar 14. Mol Ecol. 2023. PMID: 35231147 Free PMC article. Review.
Medically important bacterial-fungal interactions.
Peleg AY, Hogan DA, Mylonakis E. Peleg AY, et al. Nat Rev Microbiol. 2010 May;8(5):340-9. doi: 10.1038/nrmicro2313. Epub 2010 Mar 29. Nat Rev Microbiol. 2010. PMID: 20348933 Review.

References

1. Mycopathol Mycol Appl. 1973 Jul 31;50(3):261-9 - PubMed
1. G Batteriol Virol Immunol Microbiol. 1973 Jul-Dec;66(7-12):297-308 - PubMed
1. Anal Biochem. 1979 May;95(1):19-23 - PubMed
1. Infect Immun. 1988 Sep;56(9):2515-7 - PubMed
1. J Clin Invest. 1987 Jan;79(1):221-9 - PubMed

Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth - PubMed