Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes - PubMed

Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes

G Marbaix et al. Proc Natl Acad Sci U S A. 1975 Aug.

Abstract

Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.

Similar articles

• Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.

  Nudel U, Soreq H, Littauer UZ. Nudel U, et al. Eur J Biochem. 1976 Apr 15;64(1):115-21. doi: 10.1111/j.1432-1033.1976.tb10279.x. Eur J Biochem. 1976. PMID: 776610

• [Proceedings: Accelerated degradation of globin messenger RNA after removal of polyadenylic segment in Xenopus oocytes].

  Marbaix G, Huez G, Burny A, Cleuter Y, Hubert E, Leclercq M, Chantrenne H, Soreq H, Nudel U, Salomon R, Littauer U. Marbaix G, et al. Arch Int Physiol Biochim. 1975 May;83(2):385-6. Arch Int Physiol Biochim. 1975. PMID: 54100 French. No abstract available.

• Readenylation of polyadenylate-free globin messenger RNA restores its stability in vivo.

  Huez G, Marbaix G, Hubert E, Cleuter Y, Leclercq M, Chantrenne H, Devos R, Soreq H, Nudel U, Littauer UZ. Huez G, et al. Eur J Biochem. 1975 Nov 15;59(2):589-92. doi: 10.1111/j.1432-1033.1975.tb02486.x. Eur J Biochem. 1975. PMID: 1107033

• The Role of the poly(A) sequence in mammalian messenger RNA.

  Brawerman G. Brawerman G. CRC Crit Rev Biochem. 1981;10(1):1-38. doi: 10.3109/10409238109114634. CRC Crit Rev Biochem. 1981. PMID: 6111419 Review.

• Post-synthetic fate of the translation products of messenger RNA microinjected into Xenopus oocytes.

  Asselbergs FA. Asselbergs FA. Mol Biol Rep. 1979 Dec 31;5(4):199-208. doi: 10.1007/BF00782889. Mol Biol Rep. 1979. PMID: 393994 Review. No abstract available.

Cited by

• Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae.

  Herrick D, Parker R, Jacobson A. Herrick D, et al. Mol Cell Biol. 1990 May;10(5):2269-84. doi: 10.1128/mcb.10.5.2269-2284.1990. Mol Cell Biol. 1990. PMID: 2183028 Free PMC article.

• Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences.

  Soreq H, Sagar AD, Sehgal PB. Soreq H, et al. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1741-5. doi: 10.1073/pnas.78.3.1741. Proc Natl Acad Sci U S A. 1981. PMID: 6165016 Free PMC article.

• Determinants of mRNA stability in Dictyostelium discoideum amoebae: differences in poly(A) tail length, ribosome loading, and mRNA size cannot account for the heterogeneity of mRNA decay rates.

  Shapiro RA, Herrick D, Manrow RE, Blinder D, Jacobson A. Shapiro RA, et al. Mol Cell Biol. 1988 May;8(5):1957-69. doi: 10.1128/mcb.8.5.1957-1969.1988. Mol Cell Biol. 1988. PMID: 2898728 Free PMC article.

• Oligoadenylate is present in the mitochondrial RNA of Saccharomyces cerevisiae.

  Yuckenberg PD, Phillips SL. Yuckenberg PD, et al. Mol Cell Biol. 1982 Apr;2(4):450-6. doi: 10.1128/mcb.2.4.450-456.1982. Mol Cell Biol. 1982. PMID: 7050672 Free PMC article.

• Exogenous mRNA encoding tetanus or botulinum neurotoxins expressed in Aplysia neurons.

  Mochida S, Poulain B, Eisel U, Binz T, Kurazono H, Niemann H, Tauc L. Mochida S, et al. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7844-8. doi: 10.1073/pnas.87.20.7844. Proc Natl Acad Sci U S A. 1990. PMID: 2236000 Free PMC article.

References

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central