Quantal release of serotonin - PubMed
Quantal release of serotonin
D Bruns et al. Neuron. 2000 Oct.
Free article
Abstract
We have studied the origin of quantal variability for small synaptic vesicles (SSVs) and large dense-cored vesicles (LDCVs). As a model, we used serotonergic Retzius neurons of leech that allow for combined amperometrical and morphological analyses of quantal transmitter release. We find that the transmitter amount released by a SSV varies proportionally to the volume of the vesicle, suggesting that serotonin is stored at a constant intravesicular concentration and is completely discharged during exocytosis. Transmitter discharge from LDCVs shows a higher degree of variability than is expected from their size distribution, and bulk release from LDCVs is slower than release from SSVs. On average, differences in the transmitter amount released from SSVs and LDCVs are proportional to the size differences of the organelles, suggesting that transmitter is stored at similar concentrations in SSVs and LDCVs.
Comment in
-
Vesicles: equal in neurotransmitter concentration but not in volume.
Sulzer D, Edwards R. Sulzer D, et al. Neuron. 2000 Oct;28(1):5-7. doi: 10.1016/s0896-6273(00)00077-5. Neuron. 2000. PMID: 11086975 No abstract available.
Similar articles
-
Real-time measurement of transmitter release from single synaptic vesicles.
Bruns D, Jahn R. Bruns D, et al. Nature. 1995 Sep 7;377(6544):62-5. doi: 10.1038/377062a0. Nature. 1995. PMID: 7659162
-
Splitting the quantum: regulation of quantal release during vesicle fusion.
Burgoyne RD, Barclay JW. Burgoyne RD, et al. Trends Neurosci. 2002 Apr;25(4):176-8. doi: 10.1016/s0166-2236(00)02126-3. Trends Neurosci. 2002. PMID: 11998682 Review.
-
Henderson LP, Kuffler DP, Nicholls J, Zhang R. Henderson LP, et al. J Physiol. 1983 Jul;340:347-58. doi: 10.1113/jphysiol.1983.sp014766. J Physiol. 1983. PMID: 6310097 Free PMC article.
-
Kuffler DP, Nicholls J, Drapeau P. Kuffler DP, et al. J Comp Neurol. 1987 Feb 22;256(4):516-26. doi: 10.1002/cne.902560404. J Comp Neurol. 1987. PMID: 2435767
-
Synaptic and extrasynaptic secretion of serotonin.
De-Miguel FF, Trueta C. De-Miguel FF, et al. Cell Mol Neurobiol. 2005 Mar;25(2):297-312. doi: 10.1007/s10571-005-3061-z. Cell Mol Neurobiol. 2005. PMID: 16047543 Free PMC article. Review.
Cited by
-
Serotonin release from the neuronal cell body and its long-lasting effects on the nervous system.
De-Miguel FF, Leon-Pinzon C, Noguez P, Mendez B. De-Miguel FF, et al. Philos Trans R Soc Lond B Biol Sci. 2015 Jul 5;370(1672):20140196. doi: 10.1098/rstb.2014.0196. Philos Trans R Soc Lond B Biol Sci. 2015. PMID: 26009775 Free PMC article.
-
De-Miguel FF, Santamaría-Holek I, Noguez P, Bustos C, Hernández-Lemus E, Rubí JM. De-Miguel FF, et al. PLoS One. 2012;7(10):e45454. doi: 10.1371/journal.pone.0045454. Epub 2012 Oct 3. PLoS One. 2012. PMID: 23056204 Free PMC article.
-
Membrane transporters as mediators of synaptic dopamine dynamics: implications for disease.
Lohr KM, Masoud ST, Salahpour A, Miller GW. Lohr KM, et al. Eur J Neurosci. 2017 Jan;45(1):20-33. doi: 10.1111/ejn.13357. Epub 2016 Sep 2. Eur J Neurosci. 2017. PMID: 27520881 Free PMC article. Review.
-
Croft BG, Fortin GD, Corera AT, Edwards RH, Beaudet A, Trudeau LE, Fon EA. Croft BG, et al. Mol Biol Cell. 2005 Jan;16(1):306-15. doi: 10.1091/mbc.e04-07-0559. Epub 2004 Oct 20. Mol Biol Cell. 2005. PMID: 15496457 Free PMC article.
-
Raghupathi R, Duffield MD, Zelkas L, Meedeniya A, Brookes SJ, Sia TC, Wattchow DA, Spencer NJ, Keating DJ. Raghupathi R, et al. J Physiol. 2013 Dec 1;591(23):5959-75. doi: 10.1113/jphysiol.2013.259796. Epub 2013 Oct 7. J Physiol. 2013. PMID: 24099799 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials