Expression of nephrin in acquired human glomerular disease - PubMed
Expression of nephrin in acquired human glomerular disease
Wooseong Huh et al. Nephrol Dial Transplant. 2002 Mar.
Abstract
Background: Nephrin is a recently identified protein, which is synthesized in the podocytes and localized in the slit diaphragm area. Nephrin is a cell adhesion molecule of the immunoglobulin superfamily, and presumably is a part of the zipper-like structure of the slit membrane. As the mutation of the gene coding nephrin induces congenital nephrotic syndrome of Finnish type, which is a prototype of nephrotic syndrome, it has been suggested that nephrin also plays a role in acquired proteinuric kidney disease.
Methods: To address the above issue, the expression of nephrin in acquired human glomerular disease was studied by immunoelectron microscopy employing a polyclonal antibody against nephrin. Four normal human kidneys from nephrectomy specimens and eight kidney biopsy specimens from glomerular disease patients (one minimal change disease, one membranous glomerulonephritis (GN), one membranoproliferative GN, four IgA nephropathy, and one lupus nephritis) were studied. Proteinuria of the patients ranged from 448 to 11725 mg/day. Effacement of the foot processes was observed in all patients.
Results: The study demonstrated that the number and distribution of gold particles in the glomerular region, where the podocyte foot process was well preserved, were similar to that found in normal kidneys; however, gold particles were almost always absent in regions where the foot processes were effaced. The number of gold particles per foot process interspace was not different between normal controls and GN patients; however, the number of gold particles per defined length (1000 nm) of the glomerular basement membrane underlying the foot processes was significantly reduced in GN patients.
Conclusion: Using immunoelectron microscopy, we observed that the expression of nephrin in GN was lower in regions where the foot processes were effaced, and comparable with that of normal controls where the foot process interspaces were preserved. The significance of our observation in the context of proteinuria in acquired GN needs further clarification.
Similar articles
-
Ultrastructural study on nephrin expression in experimental puromycin aminonucleoside nephrosis.
Lee YK, Kwon T, Kim DJ, Huh W, Kim YG, Oh HY, Kawachi H. Lee YK, et al. Nephrol Dial Transplant. 2004 Dec;19(12):2981-6. doi: 10.1093/ndt/gfh489. Epub 2004 Sep 22. Nephrol Dial Transplant. 2004. PMID: 15385636
-
Kim SY, Choi YY, Kwon EJ, Seo S, Kim WY, Park SH, Park S, Chin HJ, Na KY, Kim S. Kim SY, et al. Int J Mol Sci. 2024 May 8;25(10):5121. doi: 10.3390/ijms25105121. Int J Mol Sci. 2024. PMID: 38791159 Free PMC article.
-
Targeted downregulation of extracellular nephrin in human IgA nephropathy.
Gagliardini E, Benigni A, Tomasoni S, Abbate M, Kalluri R, Remuzzi G. Gagliardini E, et al. Am J Nephrol. 2003 Jul-Aug;23(4):277-86. doi: 10.1159/000072281. Epub 2003 Jul 9. Am J Nephrol. 2003. PMID: 12853730
-
[Histological classification of chronic glomerular diseases].
Noël LH, Gubler MC. Noël LH, et al. Rev Prat. 2003 Nov 30;53(18):2005-12. Rev Prat. 2003. PMID: 15008214 Review. French.
-
[Structure and function of the glomerular filtration barrier].
Musiał K, Zwolińska D. Musiał K, et al. Pol Merkur Lekarski. 2005 Mar;18(105):317-20. Pol Merkur Lekarski. 2005. PMID: 15997642 Review. Polish.
Cited by
-
Cystinosis (ctns) zebrafish mutant shows pronephric glomerular and tubular dysfunction.
Elmonem MA, Khalil R, Khodaparast L, Khodaparast L, Arcolino FO, Morgan J, Pastore A, Tylzanowski P, Ny A, Lowe M, de Witte PA, Baelde HJ, van den Heuvel LP, Levtchenko E. Elmonem MA, et al. Sci Rep. 2017 Feb 15;7:42583. doi: 10.1038/srep42583. Sci Rep. 2017. PMID: 28198397 Free PMC article.
-
Menendez-Castro C, Nitz D, Cordasic N, Jordan J, Bäuerle T, Fahlbusch FB, Rascher W, Hilgers KF, Hartner A. Menendez-Castro C, et al. Sci Rep. 2018 Mar 14;8(1):4542. doi: 10.1038/s41598-018-22733-8. Sci Rep. 2018. PMID: 29540722 Free PMC article.
-
Tarabra E, Giunti S, Barutta F, Salvidio G, Burt D, Deferrari G, Gambino R, Vergola D, Pinach S, Perin PC, Camussi G, Gruden G. Tarabra E, et al. Diabetes. 2009 Sep;58(9):2109-18. doi: 10.2337/db08-0895. Epub 2009 Jul 8. Diabetes. 2009. PMID: 19587356 Free PMC article.
-
Nephrin Preserves Podocyte Viability and Glomerular Structure and Function in Adult Kidneys.
Li X, Chuang PY, D'Agati VD, Dai Y, Yacoub R, Fu J, Xu J, Taku O, Premsrirut PK, Holzman LB, He JC. Li X, et al. J Am Soc Nephrol. 2015 Oct;26(10):2361-77. doi: 10.1681/ASN.2014040405. Epub 2015 Feb 2. J Am Soc Nephrol. 2015. PMID: 25644109 Free PMC article.
-
Yamashita M, Yoshida T, Suzuki S, Homma K, Hayashi M. Yamashita M, et al. Clin Exp Nephrol. 2017 Feb;21(1):16-26. doi: 10.1007/s10157-016-1268-6. Epub 2016 Apr 18. Clin Exp Nephrol. 2017. PMID: 27089875
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous