pubmed.ncbi.nlm.nih.gov

Three-dimensional mapping of sensory innervation with substance p in porcine bronchial mucosa: comparison with human airways - PubMed

  • ️Tue Jan 01 2002

Comparative Study

. 2002 Nov 1;166(9):1269-81.

doi: 10.1164/rccm.2112018.

Affiliations

Comparative Study

Three-dimensional mapping of sensory innervation with substance p in porcine bronchial mucosa: comparison with human airways

Jasmine P Lamb et al. Am J Respir Crit Care Med. 2002.

Abstract

In asthma, neurogenic inflammation in bronchial airways may occur though the release of neuropeptides from C fibers via an axon reflex. Structural evidence for this neural pathway was sought in the pig and in humans by three-dimensional mapping of substance P-immunoreactive (SP-IR) nerves in whole mounts of mucosa using immunofluorescent staining and confocal microscopy. To show continuity, nerves were traced with 1,1'-didodecyl-3,3,3',3'-tetramethyl indocarbocyanine perchlorate from their epithelial endings through the mucosa. The pan-neuronal marker protein gene product 9.5 revealed an extensive apical and basal plexus of nerves in the epithelium; 94% of these were varicose SP-IR fibers. Apical SP-IR fibers had a length density of 88 mm/mm(2). Varicose apical processes followed closely around the circumference of goblet cells. Calcitonin gene-related peptide was colocalized with SP-IR in varicosites. The epithelial fibers converged into bundles as they entered the lamina propria where lateral branches ran along arterioles, often contiguous with the vascular smooth muscle. 1,1'-didodecyl-3,3,3',3'-tetramethyl indocarbocyanine perchlorate tracing showed that they projected to the epithelium. SP-IR fibers were rare near postcapillary venules. In human bronchial epithelium, protein gene product 9.5 revealed a similar apical and basal plexus of varicose fibers that weakly stained for SP-IR. Thus, a continuous sensory nerve pathway from the epithelium to arterioles provides structural support for a local axon reflex.

PubMed Disclaimer

Similar articles

Cited by

  • Sensory nerves and airway irritability.

    Canning BJ, Spina D. Canning BJ, et al. Handb Exp Pharmacol. 2009;194(194):139-83. doi: 10.1007/978-3-540-79090-7_5. Handb Exp Pharmacol. 2009. PMID: 19655107 Free PMC article. Review.

  • The porcine lung as a potential model for cystic fibrosis.

    Rogers CS, Abraham WM, Brogden KA, Engelhardt JF, Fisher JT, McCray PB Jr, McLennan G, Meyerholz DK, Namati E, Ostedgaard LS, Prather RS, Sabater JR, Stoltz DA, Zabner J, Welsh MJ. Rogers CS, et al. Am J Physiol Lung Cell Mol Physiol. 2008 Aug;295(2):L240-63. doi: 10.1152/ajplung.90203.2008. Epub 2008 May 16. Am J Physiol Lung Cell Mol Physiol. 2008. PMID: 18487356 Free PMC article. Review.

  • Role of MUC5AC in the pathogenesis of exercise-induced bronchoconstriction.

    Hallstrand TS, Debley JS, Farin FM, Henderson WR Jr. Hallstrand TS, et al. J Allergy Clin Immunol. 2007 May;119(5):1092-8. doi: 10.1016/j.jaci.2007.01.005. Epub 2007 Feb 26. J Allergy Clin Immunol. 2007. PMID: 17321575 Free PMC article.

  • An overview of the sensory receptors regulating cough.

    Mazzone SB. Mazzone SB. Cough. 2005 Aug 4;1:2. doi: 10.1186/1745-9974-1-2. Cough. 2005. PMID: 16270920 Free PMC article.

  • A temporal and spatial map of axons in developing mouse prostate.

    Turco AE, Cadena MT, Zhang HL, Sandhu JK, Oakes SR, Chathurvedula T, Peterson RE, Keast JR, Vezina CM. Turco AE, et al. Histochem Cell Biol. 2019 Jul;152(1):35-45. doi: 10.1007/s00418-019-01784-6. Epub 2019 Apr 11. Histochem Cell Biol. 2019. PMID: 30976911 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources