Validation of image analysis for enzyme histochemical and immunocytochemical staining - PubMed

Affiliations

• PMID: 12503731

Comparative Study

Validation of image analysis for enzyme histochemical and immunocytochemical staining

V M Patel et al. Biotech Histochem. 2002 Jul.

Abstract

Immunocytochemical and enzyme histochemical analyses of cells and tissues are used to detect changes in the extent of injury and the expression of various molecules. Image analysis quantitation offers an easier, more efficient technique to evaluate these changes. We studied the application of image analysis for evaluating enzyme histochemistry and immunocytochemistry of cells and tissues as a way to assess stroke. Using brain sections, we compared investigator and computer-generated image analysis of 2,3,5-triphenyltetrazolium chloride stained cerebral infarcts in rats subjected to 2 h middle cerebral artery occlusion and 22 h re-perfusion. Both methods documented the infarct volumes with a comparison of means of less then 5%. This suggests no difference between computer- and hand-calculated values. Computer-generated analysis was easier and faster to use. Using endothelial cell monolayers, immunocytochemical staining of a time course of heat shock protein expression was compared to a grading system using fast red chromagen counterstained with hematoxylin. Results demonstrated greater ease and efficiency with computer-generated image analysis compared to other subjective systems of analysis. Image analysis is more useful for detecting small differences in staining, especially when using 3,3-diaminobenzidine as a chromagen. Investigator bias is also reduced using this system. Our comparisons validate the use of this versatile technology to assess more easily both cell and tissues in stroke research.

Similar articles

• Rodent stroke induced by photochemical occlusion of proximal middle cerebral artery: evolution monitored with MR imaging and histopathology.

  Chen F, Suzuki Y, Nagai N, Jin L, Yu J, Wang H, Marchal G, Ni Y. Chen F, et al. Eur J Radiol. 2007 Jul;63(1):68-75. doi: 10.1016/j.ejrad.2007.01.005. Epub 2007 Mar 6. Eur J Radiol. 2007. PMID: 17337149

• Semi-automated image processing system for micro- to macro-scale analysis of immunohistopathology: application to ischemic brain tissue.

  Wu C, Zhao W, Lin B, Ginsberg MD. Wu C, et al. Comput Methods Programs Biomed. 2005 Apr;78(1):75-86. doi: 10.1016/j.cmpb.2004.12.005. Comput Methods Programs Biomed. 2005. PMID: 15780892

• Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats.

  Tsubokawa T, Yamaguchi-Okada M, Calvert JW, Solaroglu I, Shimamura N, Yata K, Zhang JH. Tsubokawa T, et al. J Neurosci Res. 2006 Sep;84(4):832-40. doi: 10.1002/jnr.20977. J Neurosci Res. 2006. PMID: 16802320

• Quantitation in immunohistochemistry. A research method or a diagnostic tool in surgical pathology?

  Fritz P, Wu X, Tuczek H, Multhaupt H, Schwarzmann P. Fritz P, et al. Pathologica. 1995 Jun;87(3):300-9. Pathologica. 1995. PMID: 8570290 Review.

• [Quantitative immunohistochemistry: optimization of analyzing the expression of signaling molecules in the myometrium].

  Smirnova MIu, Dzhemlikhanova LKh, Kostiuchek IN, Kvetnoĭ IM, Nauri DA, Pal'tsev MA. Smirnova MIu, et al. Arkh Patol. 2010 Jan-Feb;72(1):51-5. Arkh Patol. 2010. PMID: 20369589 Review. Russian.

Cited by

• A Low-Protein, High-Carbohydrate Diet Stimulates Thermogenesis in the Brown Adipose Tissue of Rats via ATF-2.

  de França SA, dos Santos MP, Przygodda F, Garófalo MA, Kettelhut IC, Magalhães DA, Bezerra KS, Colodel EM, Flouris AD, Andrade CM, Kawashita NH. de França SA, et al. Lipids. 2016 Mar;51(3):303-10. doi: 10.1007/s11745-016-4119-z. Epub 2016 Jan 19. Lipids. 2016. PMID: 26781764

Publication types

MeSH terms

Substances