Conversion of 48S translation preinitiation complexes into 80S initiation complexes as revealed by toeprinting - PubMed

Conversion of 48S translation preinitiation complexes into 80S initiation complexes as revealed by toeprinting

Sergey E Dmitriev et al. FEBS Lett. 2003.

Free article

Abstract

A method of analysis of translation initiation complexes by toeprinting has recently acquired a wide application to investigate molecular mechanisms of translation initiation in eukaryotes. So far, this very fruitful approach was used when researchers did not aim to discriminate between patterns of toeprints for 48S and 80S translation initiation complexes. Here, using cap-dependent and internal ribosomal entry site (IRES)-dependent mRNAs, we show that the toeprint patterns for 48S and 80S complexes are distinct whether the complexes are assembled in rabbit reticulocyte lysate or from fully purified individual components. This observation allowed us to demonstrate for the first time a delay in the conversion of the 48S complex into the 80S complex for beta-globin and encephalomyocarditis virus (EMCV) RNAs, and to assess the potential of some 80S antibiotics to block polypeptide elongation. Besides, additional selection of the authentic initiation codon among three consecutive AUGs that follow the EMCV IRES was revealed at steps subsequent to the location of the initiation codon by the 40S ribosomal subunit.

Similar articles

• Assembly of 48S translation initiation complexes from purified components with mRNAs that have some base pairing within their 5' untranslated regions.

  Dmitriev SE, Terenin IM, Dunaevsky YE, Merrick WC, Shatsky IN. Dmitriev SE, et al. Mol Cell Biol. 2003 Dec;23(24):8925-33. doi: 10.1128/MCB.23.24.8925-8933.2003. Mol Cell Biol. 2003. PMID: 14645505 Free PMC article.

• Reconstitution of mammalian 48S ribosomal translation initiation complex.

  Majumdar R, Chaudhuri J, Maitra U. Majumdar R, et al. Methods Enzymol. 2007;430:179-208. doi: 10.1016/S0076-6879(07)30008-6. Methods Enzymol. 2007. PMID: 17913639

• Exploring accessibility of structural elements of the mammalian 40S ribosomal mRNA entry channel at various steps of translation initiation.

  Sharifulin DE, Bartuli YS, Meschaninova MI, Ven'yaminova AG, Graifer DM, Karpova GG. Sharifulin DE, et al. Biochim Biophys Acta. 2016 Oct;1864(10):1328-38. doi: 10.1016/j.bbapap.2016.06.011. Epub 2016 Jun 23. Biochim Biophys Acta. 2016. PMID: 27346718

• Molecular mechanisms of translation initiation in eukaryotes.

  Pestova TV, Kolupaeva VG, Lomakin IB, Pilipenko EV, Shatsky IN, Agol VI, Hellen CU. Pestova TV, et al. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7029-36. doi: 10.1073/pnas.111145798. Proc Natl Acad Sci U S A. 2001. PMID: 11416183 Free PMC article. Review.

• Translation of hepatitis C virus RNA.

  Hellen CU, Pestova TV. Hellen CU, et al. J Viral Hepat. 1999 Mar;6(2):79-87. doi: 10.1046/j.1365-2893.1999.00150.x. J Viral Hepat. 1999. PMID: 10607219 Review.

Cited by

• Glycyl-tRNA synthetase specifically binds to the poliovirus IRES to activate translation initiation.

  Andreev DE, Hirnet J, Terenin IM, Dmitriev SE, Niepmann M, Shatsky IN. Andreev DE, et al. Nucleic Acids Res. 2012 Jul;40(12):5602-14. doi: 10.1093/nar/gks182. Epub 2012 Feb 28. Nucleic Acids Res. 2012. PMID: 22373920 Free PMC article.

• A Nascent Peptide Signal Responsive to Endogenous Levels of Polyamines Acts to Stimulate Regulatory Frameshifting on Antizyme mRNA.

  Yordanova MM, Wu C, Andreev DE, Sachs MS, Atkins JF. Yordanova MM, et al. J Biol Chem. 2015 Jul 17;290(29):17863-17878. doi: 10.1074/jbc.M115.647065. Epub 2015 May 21. J Biol Chem. 2015. PMID: 25998126 Free PMC article.

• Assembly of 48S translation initiation complexes from purified components with mRNAs that have some base pairing within their 5' untranslated regions.

  Dmitriev SE, Terenin IM, Dunaevsky YE, Merrick WC, Shatsky IN. Dmitriev SE, et al. Mol Cell Biol. 2003 Dec;23(24):8925-33. doi: 10.1128/MCB.23.24.8925-8933.2003. Mol Cell Biol. 2003. PMID: 14645505 Free PMC article.

• Translation factors promote the formation of two states of the closed-loop mRNP.

  Amrani N, Ghosh S, Mangus DA, Jacobson A. Amrani N, et al. Nature. 2008 Jun 26;453(7199):1276-80. doi: 10.1038/nature06974. Epub 2008 May 21. Nature. 2008. PMID: 18496529 Free PMC article.

• Translation initiation of alphavirus mRNA reveals new insights into the topology of the 48S initiation complex.

  Toribio R, Díaz-López I, Boskovic J, Ventoso I. Toribio R, et al. Nucleic Acids Res. 2018 May 4;46(8):4176-4187. doi: 10.1093/nar/gky071. Nucleic Acids Res. 2018. PMID: 29415133 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science
  • Wiley

• Miscellaneous

  • NCI CPTAC Assay Portal