Spatial and temporal organization of replicating Escherichia coli chromosomes - PubMed
Spatial and temporal organization of replicating Escherichia coli chromosomes
Ivy F Lau et al. Mol Microbiol. 2003 Aug.
Free article
Abstract
The positions of DNA regions close to the chromosome replication origin and terminus in growing cells of Escherichia coli have been visualized simultaneously, using new widely applicable reagents. Furthermore, the positions of these regions with respect to a replication factory-associated protein have been analysed. Time-lapse analysis has allowed the fate of origins, termini and the FtsZ ring to be followed in a lineage-specific manner during the formation of microcolonies. These experiments reveal new aspects of the E. coli cell cycle and demonstrate that the replication terminus region is frequently located asymmetrically, on the new pole side of mid-cell. This asymmetry could provide a mechanism by which the chromosome segregation protein FtsK, located at the division septum, can act directionally to ensure that the septal region is free of DNA before the completion of cell division.
Similar articles
-
FtsK actively segregates sister chromosomes in Escherichia coli.
Stouf M, Meile JC, Cornet F. Stouf M, et al. Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11157-62. doi: 10.1073/pnas.1304080110. Epub 2013 Jun 18. Proc Natl Acad Sci U S A. 2013. PMID: 23781109 Free PMC article.
-
Dancing around the divisome: asymmetric chromosome segregation in Escherichia coli.
Wang X, Possoz C, Sherratt DJ. Wang X, et al. Genes Dev. 2005 Oct 1;19(19):2367-77. doi: 10.1101/gad.345305. Genes Dev. 2005. PMID: 16204186 Free PMC article.
-
The segregation of the Escherichia coli origin and terminus of replication.
Li Y, Sergueev K, Austin S. Li Y, et al. Mol Microbiol. 2002 Nov;46(4):985-96. doi: 10.1046/j.1365-2958.2002.03234.x. Mol Microbiol. 2002. PMID: 12421305
-
FtsK, a literate chromosome segregation machine.
Bigot S, Sivanathan V, Possoz C, Barre FX, Cornet F. Bigot S, et al. Mol Microbiol. 2007 Jun;64(6):1434-41. doi: 10.1111/j.1365-2958.2007.05755.x. Epub 2007 May 18. Mol Microbiol. 2007. PMID: 17511809 Review.
-
Host controlled plasmid replication: Escherichia coli minichromosomes.
Dasgupta S, Løbner-Olesen A. Dasgupta S, et al. Plasmid. 2004 Nov;52(3):151-68. doi: 10.1016/j.plasmid.2004.08.001. Plasmid. 2004. PMID: 15518873 Review.
Cited by
-
Four-dimensional imaging of E. coli nucleoid organization and dynamics in living cells.
Fisher JK, Bourniquel A, Witz G, Weiner B, Prentiss M, Kleckner N. Fisher JK, et al. Cell. 2013 May 9;153(4):882-95. doi: 10.1016/j.cell.2013.04.006. Epub 2013 Apr 25. Cell. 2013. PMID: 23623305 Free PMC article.
-
CRISPR-mediated control of the bacterial initiation of replication.
Wiktor J, Lesterlin C, Sherratt DJ, Dekker C. Wiktor J, et al. Nucleic Acids Res. 2016 May 5;44(8):3801-10. doi: 10.1093/nar/gkw214. Epub 2016 Apr 1. Nucleic Acids Res. 2016. PMID: 27036863 Free PMC article.
-
Peterson E, Söderström B, Prins N, Le GHB, Hartley-Tassell LE, Evenhuis C, Grønnemose RB, Andersen TE, Møller-Jensen J, Iosifidis G, Duggin IG, Saunders B, Harry EJ, Bottomley AL. Peterson E, et al. PLoS Pathog. 2024 Sep 6;20(9):e1012458. doi: 10.1371/journal.ppat.1012458. eCollection 2024 Sep. PLoS Pathog. 2024. PMID: 39241059 Free PMC article.
-
Direct observation of independently moving replisomes in Escherichia coli.
Japaridze A, Gogou C, Kerssemakers JWJ, Nguyen HM, Dekker C. Japaridze A, et al. Nat Commun. 2020 Jun 19;11(1):3109. doi: 10.1038/s41467-020-16946-7. Nat Commun. 2020. PMID: 32561741 Free PMC article.
-
Mapping the driving forces of chromosome structure and segregation in Escherichia coli.
Kuwada NJ, Cheveralls KC, Traxler B, Wiggins PA. Kuwada NJ, et al. Nucleic Acids Res. 2013 Aug;41(15):7370-7. doi: 10.1093/nar/gkt468. Epub 2013 Jun 17. Nucleic Acids Res. 2013. PMID: 23775792 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases