Identification and molecular analysis of glgS, a novel growth-phase-regulated and rpoS-dependent gene involved in glycogen synthesis in Escherichia coli - PubMed

Identification and molecular analysis of glgS, a novel growth-phase-regulated and rpoS-dependent gene involved in glycogen synthesis in Escherichia coli

R Hengge-Aronis et al. Mol Microbiol. 1992 Jul.

Abstract

The putative stationary-phase sigma factor (sigma S) encoded by rpoS is essential for glycogen synthesis, but is not required for the transcription of glgC and glgA, which encode ADP-glucose-pyrophosphorylase and glycogen synthase, respectively. Using a mini-Mu random chromosomal library and a screen for glycogen overproduction, we identified a novel gene (glgS) involved in glycogen synthesis. glgS maps at 66.6 min (3247 kb) on the chromosome and constitutes a monocistronic operon. It encodes a hydrophilic and highly charged small protein, with a molecular weight of 7886, which is strongly expressed in minicells. Experiments with single-copy chromosomal glgS::lacZ gene fusions indicated that glgS expression is controlled by sigma S as well as by cAMP. Two transcriptional start sites were mapped in the upstream regulatory region of glgS. The glgSp1 transcript was absent in a cya mutant, whereas an rpoS mutant did not synthesize the glgSp2 transcript. Although glycogen synthesis is strongly stimulated by overproduction of GlgS and is inhibited by a glgS null mutation, glgS does not affect the expression of the glgCAP operon. Its potential role in the metabolic control of glycogen synthesis is discussed. Also, evidence is presented to show that the amount of glycogen accumulated in vivo in early stationary-phase cells is mainly determined by sigma S-controlled gene expression and allosteric activation of GlgC, whereas the absolute levels of expression of glgCAP as well as the intracellular concentration of cAMP are of minor importance.

Similar articles

• Stationary phase-specific expression of the fic gene in Escherichia coli K-12 is controlled by the rpoS gene product (sigma 38).

  Utsumi R, Kusafuka S, Nakayama T, Tanaka K, Takayanagi Y, Takahashi H, Noda M, Kawamukai M. Utsumi R, et al. FEMS Microbiol Lett. 1993 Nov 1;113(3):273-8. doi: 10.1111/j.1574-6968.1993.tb06526.x. FEMS Microbiol Lett. 1993. PMID: 8270191

• GlgS, described previously as a glycogen synthesis control protein, negatively regulates motility and biofilm formation in Escherichia coli.

  Rahimpour M, Montero M, Almagro G, Viale AM, Sevilla Á, Cánovas M, Muñoz FJ, Baroja-Fernández E, Bahaji A, Eydallin G, Dose H, Takeuchi R, Mori H, Pozueta-Romero J. Rahimpour M, et al. Biochem J. 2013 Jun 15;452(3):559-73. doi: 10.1042/BJ20130154. Biochem J. 2013. PMID: 23537328

• Identification and characterization of stationary phase-inducible genes in Escherichia coli.

  Weichart D, Lange R, Henneberg N, Hengge-Aronis R. Weichart D, et al. Mol Microbiol. 1993 Oct;10(2):407-20. Mol Microbiol. 1993. PMID: 7934831

• Regulation of bacterial glycogen synthesis.

  Preiss J, Yung SG, Baecker PA. Preiss J, et al. Mol Cell Biochem. 1983;57(1):61-80. doi: 10.1007/BF00223525. Mol Cell Biochem. 1983. PMID: 6316123 Review.

• Post-transcriptional regulation of bacterial carbohydrate metabolism: evidence that the gene product CsrA is a global mRNA decay factor.

  Romeo T. Romeo T. Res Microbiol. 1996 Jul-Sep;147(6-7):505-12. doi: 10.1016/0923-2508(96)84004-6. Res Microbiol. 1996. PMID: 9084762 Review. No abstract available.

Cited by

• The General Stress Response Is Conserved in Long-Term Soil-Persistent Strains of Escherichia coli.

  Somorin Y, Abram F, Brennan F, O'Byrne C. Somorin Y, et al. Appl Environ Microbiol. 2016 Jul 15;82(15):4628-4640. doi: 10.1128/AEM.01175-16. Print 2016 Aug 1. Appl Environ Microbiol. 2016. PMID: 27235429 Free PMC article.

• The RpoS-mediated general stress response in Escherichia coli.

  Battesti A, Majdalani N, Gottesman S. Battesti A, et al. Annu Rev Microbiol. 2011;65:189-213. doi: 10.1146/annurev-micro-090110-102946. Annu Rev Microbiol. 2011. PMID: 21639793 Free PMC article. Review.

• Nanocalorimetry Reveals the Growth Dynamics of Escherichia coli Cells Undergoing Adaptive Evolution during Long-Term Stationary Phase.

  Robador A, Amend JP, Finkel SE. Robador A, et al. Appl Environ Microbiol. 2019 Jul 18;85(15):e00968-19. doi: 10.1128/AEM.00968-19. Print 2019 Aug 1. Appl Environ Microbiol. 2019. PMID: 31152016 Free PMC article.

• Sigma s-dependent promoters in Escherichia coli are located in DNA regions with intrinsic curvature.

  Espinosa-Urgel M, Tormo A. Espinosa-Urgel M, et al. Nucleic Acids Res. 1993 Aug 11;21(16):3667-70. doi: 10.1093/nar/21.16.3667. Nucleic Acids Res. 1993. PMID: 8367283 Free PMC article.

• Coordinate genetic regulation of glycogen catabolism and biosynthesis in Escherichia coli via the CsrA gene product.

  Yang H, Liu MY, Romeo T. Yang H, et al. J Bacteriol. 1996 Feb;178(4):1012-7. doi: 10.1128/jb.178.4.1012-1017.1996. J Bacteriol. 1996. PMID: 8576033 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology

• Research Materials

  • NCI CPTC Antibody Characterization Program