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Homology of a vesicular amine transporter to a gene conferring resistance to 1-methyl-4-phenylpyridinium - PubMed

️Wed Jan 01 1992

Comparative Study

Homology of a vesicular amine transporter to a gene conferring resistance to 1-methyl-4-phenylpyridinium

Y Stern-Bach et al. Proc Natl Acad Sci U S A. 1992.

Abstract

The vesicular amine transporter (VAT) catalyzes transport and storage of catechol and indolamines into subcellular organelles in a wide variety of cells. It plays a central role in neurotransmission and is the primary target for several pharmacological agents. One of the drugs, reserpine, binds very tightly to the transporter and remains bound even after solubilization, a finding that has proven useful for purification of the transporter from bovine adrenal medulla in a fully functional state. The sequences of 26 N-terminal amino acids and of an additional 7-amino acid internal peptide are presented. Antibodies against a synthetic peptide based on the above sequences immunoprecipitate the transporter, confirming the conclusion that the peptide sequence is derived from bovine VAT. To our knowledge, documentation of sequences of vesicular neurotransmitter transporters has not been presented previously. In addition, the sequences obtained are highly homologous to the predicted sequence of a protein from PC12 cells that confers to Chinese hamster ovary cells resistance to 1-methyl-4-phenylpyridinium (MPP+), an agent that causes parkinsonism in model systems, confirming the hypothesis that the protein conferring resistance to MPP+ is a VAT.

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References

1. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3713-6 - PubMed
1. J Biol Chem. 1962 Jul;237:2311-7 - PubMed
1. Biochemistry. 1992 Feb 25;31(7):1980-6 - PubMed
1. J Biol Chem. 1990 Mar 5;265(7):3961-6 - PubMed
1. J Bioenerg Biomembr. 1989 Oct;21(5):605-20 - PubMed

Homology of a vesicular amine transporter to a gene conferring resistance to 1-methyl-4-phenylpyridinium - PubMed