DIFFERENTIATION OF PATHOGENIC AND SAPROPHYTIC LEPTOSPIRES WITH 8-AZAGUANINE - PubMed

DIFFERENTIATION OF PATHOGENIC AND SAPROPHYTIC LEPTOSPIRES WITH 8-AZAGUANINE

R C JOHNSON et al. J Bacteriol. 1964 Dec.

Abstract

Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618-1623. 1964.-The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospires was investigated. Growth of strains of the saprophyte Leptospira biflexa was almost insensitive to the bacteriostatic action of 8-azaguanine at concentrations varying from 25 to 600 mug/ml; these saprophytic leptospires were serially transferred five times in media containing 225 mug without any change in growth rate or cell yield. In contrast, decreased growth rate and cell yield of the pathogenic serotypes were observed with 25 to 50 mug/ml of 8-azaguanine. Complete inhibition of growth occurred at concentrations of 100 mug/ml and above. A medium containing 225 mug/ml of 8-azaguanine was successfully used to differentiate 20 serotypes of pathogenic leptospires and 10 saprophytic strains. L. andaman CH11, L. semarang Veldrat S1 73, and L. andaman Correa, were classified with the L. biflexa strains on the basis of their growth response to 8-azaguanine.

Similar articles

• 5-FLUOROURACIL AS A SELECTIVE AGENT FOR GROWTH OF LEPTOSPIRAE.

  JOHNSON RC, ROGERS P. JOHNSON RC, et al. J Bacteriol. 1964 Feb;87(2):422-6. doi: 10.1128/jb.87.2.422-426.1964. J Bacteriol. 1964. PMID: 14151066 Free PMC article.

• Global proteome of the saprophytic strain Leptospira biflexa and comparative analysis with pathogenic strain Leptospira interrogans uncover new pathogenesis mechanisms.

  Nascimento Filho EG, Vieira ML, Dias M, Mendes MA, Sanchez FB, Setubal JC, Heinemann MB, Souza GO, Pimenta DC, Nascimento ALTO. Nascimento Filho EG, et al. J Proteomics. 2024 Apr 15;297:105125. doi: 10.1016/j.jprot.2024.105125. Epub 2024 Feb 14. J Proteomics. 2024. PMID: 38364905

• LEPTOSPIRAL COLONIAL MORPHOLOGY.

  STALHEIM OH, WILSON JB. STALHEIM OH, et al. J Bacteriol. 1963 Sep;86(3):482-9. doi: 10.1128/jb.86.3.482-489.1963. J Bacteriol. 1963. PMID: 14066425 Free PMC article.

• [SOME DIFFERENCES BETWEEN PATHOGENIC LEPTOSPIRA AND SAPROPHYTIC LEPTOSPIRA].

  KOLOCHINE-ERBER B, MAILLOUX M. KOLOCHINE-ERBER B, et al. Biol Med (Paris). 1963 Sep-Oct;52:496-504. Biol Med (Paris). 1963. PMID: 14085953 Review. French. No abstract available.

• Biofilm formation and social interaction of Leptospira in natural and artificial environments.

  Meganathan Y, Vishwakarma A, Ramya M. Meganathan Y, et al. Res Microbiol. 2022 Oct-Dec;173(8):103981. doi: 10.1016/j.resmic.2022.103981. Epub 2022 Aug 1. Res Microbiol. 2022. PMID: 35926730 Review.

Cited by

• Differentiation of pathogenic and saprophytic letospires. I. Growth at low temperatures.

  Johnson RC, Harris VG. Johnson RC, et al. J Bacteriol. 1967 Jul;94(1):27-31. doi: 10.1128/jb.94.1.27-31.1967. J Bacteriol. 1967. PMID: 6027998 Free PMC article.

• Leptospirosis: a neglected tropical zoonotic infection of public health importance-an updated review.

  Karpagam KB, Ganesh B. Karpagam KB, et al. Eur J Clin Microbiol Infect Dis. 2020 May;39(5):835-846. doi: 10.1007/s10096-019-03797-4. Epub 2020 Jan 2. Eur J Clin Microbiol Infect Dis. 2020. PMID: 31898795 Review.

• Leptospira mayottensis sp. nov., a pathogenic species of the genus Leptospira isolated from humans.

  Bourhy P, Collet L, Brisse S, Picardeau M. Bourhy P, et al. Int J Syst Evol Microbiol. 2014 Dec;64(Pt 12):4061-4067. doi: 10.1099/ijs.0.066597-0. Epub 2014 Sep 23. Int J Syst Evol Microbiol. 2014. PMID: 25249563 Free PMC article.

• Persistence of Leptospira borgpetersenii Serovar Hardjo in Refrigerated Raw Milk: A Transmission Risk of Leptospirosis to Humans.

  Mazzotta E, Ceglie L, Giurisato I, Bellinati L, Lucchese L, Marchione S, Natale A. Mazzotta E, et al. Pathogens. 2021 Mar 3;10(3):291. doi: 10.3390/pathogens10030291. Pathogens. 2021. PMID: 33802583 Free PMC article.

• Antileptospiral activity in lower-vertebrate sera.

  Charon NW, Johnson RC, Muschel LH. Charon NW, et al. Infect Immun. 1975 Dec;12(6):1386-91. doi: 10.1128/iai.12.6.1386-1391.1975. Infect Immun. 1975. PMID: 1081972 Free PMC article.

References

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Miscellaneous

  • NCI CPTAC Assay Portal