pubmed.ncbi.nlm.nih.gov

Regulation of isopropylmalate isomerase synthesis in Neurospora crassa - PubMed

Regulation of isopropylmalate isomerase synthesis in Neurospora crassa

V E Reichenbecher Jr et al. J Bacteriol. 1978 Feb.

Abstract

The capacity to synthetize isopropylmalate isomerase (EC 4.2.1.33) by Neurospora crassa increased during induction in the presence of cycloheximide but was inhibited by proflavine and other inhibitors of RNA synthesis. Turnover of the enzyme once formed appeared negligible, but the message (measured as enzyme-forming capacity) had a half-life of 4 to 8 min. A comparison of the kinetics of induction in the wild type and a newly isolated alpha-isopropylmalate-permeable strain suggested strongly that feedback control by leucine of alpha-isopropylmalate production can adequately serve as the primary physiological regulator of endogenous inducer concentration. Genetic data are presented which implicate the involvement of two unlinked genes, ipm-1 and ipm-2, in determining permeation of alpha-isopropylmalate.

PubMed Disclaimer

Similar articles

Cited by

References

    1. J Mol Biol. 1961 Feb;3:18-30 - PubMed
    1. Biochemistry. 1963 Sep-Oct;2:1046-52 - PubMed
    1. Biochim Biophys Acta. 1963 Oct 15;76:293-309 - PubMed
    1. J Bacteriol. 1978 Feb;133(2):802-10 - PubMed
    1. Genetics. 1954 Sep;39(5):604-18 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources