Development of high-sensitivity ion trap ion mobility spectrometry time-of-flight techniques: a high-throughput nano-LC-IMS-TOF separation of peptides arising from a Drosophila protein extract - PubMed

Affiliations

• PMID: 14708788
• DOI: 10.1021/ac030107f

Development of high-sensitivity ion trap ion mobility spectrometry time-of-flight techniques: a high-throughput nano-LC-IMS-TOF separation of peptides arising from a Drosophila protein extract

Sunnie Myung et al. Anal Chem. 2003.

Abstract

A linear octopole trap interface for an ion mobility time-of-flight mass spectrometer has been developed for focusing and accumulating continuous beams of ions produced by electrospray ionization. The interface improves experimental efficiencies by factors of approximately 50-200 compared with an analogous configuration that utilizes a three-dimensional Paul geometry trap (Hoaglund-Hyzer, C. S.; Lee, Y. J.; Counterman, A. E.; Clemmer, D. E. Anal. Chem. 2002, 74, 992-1006). With these improvements, it is possible to record nested drift (flight) time distributions for complex mixtures in fractions of a second. We demonstrate the approach for several well-defined peptide mixtures and an assessment of the detection limits is given. Additionally, we demonstrate the utility of the approach in the field of proteomics by an on-line, three-dimensional nano-LC-ion mobility-TOF separation of tryptic peptides from the Drosophila proteome.

Similar articles

• Coupling ion mobility separations, collisional activation techniques, and multiple stages of MS for analysis of complex peptide mixtures.

  Hoaglund-Hyzer CS, Lee YJ, Counterman AE, Clemmer DE. Hoaglund-Hyzer CS, et al. Anal Chem. 2002 Mar 1;74(5):992-1006. doi: 10.1021/ac010837s. Anal Chem. 2002. PMID: 11925002

• Rapid separation and quantitative analysis of peptides using a new nanoelectrospray- differential mobility spectrometer-mass spectrometer system.

  Levin DS, Miller RA, Nazarov EG, Vouros P. Levin DS, et al. Anal Chem. 2006 Aug 1;78(15):5443-52. doi: 10.1021/ac060003f. Anal Chem. 2006. PMID: 16878881

• Triple quadrupole linear ion trap mass spectrometer for the analysis of small molecules and macromolecules.

  Hopfgartner G, Varesio E, Tschäppät V, Grivet C, Bourgogne E, Leuthold LA. Hopfgartner G, et al. J Mass Spectrom. 2004 Aug;39(8):845-55. doi: 10.1002/jms.659. J Mass Spectrom. 2004. PMID: 15329837 Review.

• Development of high throughput dispersive LC-ion mobility-TOFMS techniques for analysing the human plasma proteome.

  Liu X, Plasencia M, Ragg S, Valentine SJ, Clemmer DE. Liu X, et al. Brief Funct Genomic Proteomic. 2004 Aug;3(2):177-86. doi: 10.1093/bfgp/3.2.177. Brief Funct Genomic Proteomic. 2004. PMID: 15355599 Review.

Cited by

• Two-dimensional gas-phase separations coupled to mass spectrometry for analysis of complex mixtures.

  Tang K, Li F, Shvartsburg AA, Strittmatter EF, Smith RD. Tang K, et al. Anal Chem. 2005 Oct 1;77(19):6381-8. doi: 10.1021/ac050871x. Anal Chem. 2005. PMID: 16194103 Free PMC article.

• Mapping Biological Networks from Quantitative Data-Independent Acquisition Mass Spectrometry: Data to Knowledge Pipelines.

  Crowgey EL, Matlock A, Venkatraman V, Fert-Bober J, Van Eyk JE. Crowgey EL, et al. Methods Mol Biol. 2017;1558:395-413. doi: 10.1007/978-1-4939-6783-4_19. Methods Mol Biol. 2017. PMID: 28150249 Free PMC article.

• Feasibility of higher-order differential ion mobility separations using new asymmetric waveforms.

  Shvartsburg AA, Mashkevich SV, Smith RD. Shvartsburg AA, et al. J Phys Chem A. 2006 Mar 2;110(8):2663-73. doi: 10.1021/jp055349t. J Phys Chem A. 2006. PMID: 16494377 Free PMC article.

• Examining the Influence of Phosphorylation on Peptide Ion Structure by Ion Mobility Spectrometry-Mass Spectrometry.

  Glover MS, Dilger JM, Acton MD, Arnold RJ, Radivojac P, Clemmer DE. Glover MS, et al. J Am Soc Mass Spectrom. 2016 May;27(5):786-94. doi: 10.1007/s13361-016-1343-y. Epub 2016 Feb 9. J Am Soc Mass Spectrom. 2016. PMID: 26860087 Free PMC article.

• Multiplexed MS/MS for improved data-independent acquisition.

  Egertson JD, Kuehn A, Merrihew GE, Bateman NW, MacLean BX, Ting YS, Canterbury JD, Marsh DM, Kellmann M, Zabrouskov V, Wu CC, MacCoss MJ. Egertson JD, et al. Nat Methods. 2013 Aug;10(8):744-6. doi: 10.1038/nmeth.2528. Epub 2013 Jun 23. Nat Methods. 2013. PMID: 23793237 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • American Chemical Society

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • FlyBase