Characterization of dental epithelial progenitor cells derived from cervical-loop epithelium in a rat lower incisor - PubMed

Comparative Study

Characterization of dental epithelial progenitor cells derived from cervical-loop epithelium in a rat lower incisor

S Kawano et al. J Dent Res. 2004 Feb.

Abstract

Dental epithelial progenitor cells differentiate into various cell types during development of tooth germs. To study this mechanism, we produced immortalized dental epithelial progenitor cells derived from the cervical-loop epithelium of a rat lower incisor. The expression patterns of cytokeratin 14, nerve growth factor receptor p75, amelogenin, Notch2, and alkaline phosphatase were examined by immunohistochemistry in both lower and higher cell densities. The patterns of each were compared in the dental epithelium of rat lower incisors. The results demonstrated that these cells could produce ameloblast lineage cells, stratum intermedium cells, stellate reticulum, and outer enamel epithelium. Furthermore, fibroblast growth factor 10 stimulated proliferation of dental progenitor cells and subsequently increased the number of cells expressing alkaline phosphatase. These results suggest that fibroblast growth factor 10 plays a role in coupling mitogenesis of the cervical-loop cells and the production of stratum intermedium cells in rat incisors.

Similar articles

• In vitro differentiation of dental epithelial progenitor cells through epithelial-mesenchymal interactions.

  Morotomi T, Kawano S, Toyono T, Kitamura C, Terashita M, Uchida T, Toyoshima K, Harada H. Morotomi T, et al. Arch Oral Biol. 2005 Aug;50(8):695-705. doi: 10.1016/j.archoralbio.2004.12.006. Epub 2005 Feb 26. Arch Oral Biol. 2005. PMID: 15958201

• Expression of cytokeratin 14 in ameloblast-lineage cells of the developing tooth of rat, both in vivo and in vitro.

  Tabata MJ, Matsumura T, Liu JG, Wakisaka S, Kurisu K. Tabata MJ, et al. Arch Oral Biol. 1996 Nov;41(11):1019-27. doi: 10.1016/s0003-9969(96)00087-8. Arch Oral Biol. 1996. PMID: 9068866

• Expression of DCC in differentiating ameloblasts from developing tooth germs in rats.

  Kim HJ, Jeon SK, Kang JH, Kim MS, Ko HM, Jung JY, Koh JT, Kim WJ, Lee EJ, Lim HP, Kim SH. Kim HJ, et al. Arch Oral Biol. 2009 Jun;54(6):563-9. doi: 10.1016/j.archoralbio.2009.03.003. Epub 2009 Apr 11. Arch Oral Biol. 2009. PMID: 19362703

• Localization of putative stem cells in dental epithelium and their association with Notch and FGF signaling.

  Harada H, Kettunen P, Jung HS, Mustonen T, Wang YA, Thesleff I. Harada H, et al. J Cell Biol. 1999 Oct 4;147(1):105-20. doi: 10.1083/jcb.147.1.105. J Cell Biol. 1999. PMID: 10508859 Free PMC article.

• Enamel knots as signaling centers linking tooth morphogenesis and odontoblast differentiation.

  Thesleff I, Keränen S, Jernvall J. Thesleff I, et al. Adv Dent Res. 2001 Aug;15:14-8. doi: 10.1177/08959374010150010401. Adv Dent Res. 2001. PMID: 12640732 Review.

Cited by

• Biphasic Functions of Sodium Fluoride (NaF) in Soft and in Hard Periodontal Tissues.

  Wang X, Tewari N, Sato F, Tanimoto K, Thangavelu L, Makishima M, Bhawal UK. Wang X, et al. Int J Mol Sci. 2022 Jan 16;23(2):962. doi: 10.3390/ijms23020962. Int J Mol Sci. 2022. PMID: 35055148 Free PMC article. Review.

• Successful reconstruction of tooth germ with cell lines requires coordinated gene expressions from the initiation stage.

  Komine A, Tomooka Y. Komine A, et al. Cells. 2012 Oct 30;1(4):905-25. doi: 10.3390/cells1040905. Cells. 2012. PMID: 24710535 Free PMC article.

• Globoside accelerates the differentiation of dental epithelial cells into ameloblasts.

  Nakamura T, Chiba Y, Naruse M, Saito K, Harada H, Fukumoto S. Nakamura T, et al. Int J Oral Sci. 2016 Dec 16;8(4):205-212. doi: 10.1038/ijos.2016.35. Int J Oral Sci. 2016. PMID: 27767053 Free PMC article.

• Daughters of the Enamel Organ: Development, Fate, and Function of the Stratum Intermedium, Stellate Reticulum, and Outer Enamel Epithelium.

  Liu H, Yan X, Pandya M, Luan X, Diekwisch TG. Liu H, et al. Stem Cells Dev. 2016 Oct;25(20):1580-1590. doi: 10.1089/scd.2016.0267. Epub 2016 Sep 9. Stem Cells Dev. 2016. PMID: 27611344 Free PMC article.

• ROS-Mediated Enamel Formation Disturbance Characterized by Alternative Cervical Loop Cell Proliferation and Downregulation of RhoA/ROCK in Ameloblasts.

  Xu Y, Zhang Y, Zheng J, Xu M, Yang Y, Guo W. Xu Y, et al. Oxid Med Cell Longev. 2022 Oct 17;2022:5769679. doi: 10.1155/2022/5769679. eCollection 2022. Oxid Med Cell Longev. 2022. PMID: 36299607 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal