Doxorubicin accumulation in individually electrophoresed organelles - PubMed

Affiliations

• PMID: 15281791
• DOI: 10.1021/ja0492539

Doxorubicin accumulation in individually electrophoresed organelles

Adrian B Anderson et al. J Am Chem Soc. 2004.

Abstract

We report the doxorubicin content in individual organelles following their capillary electrophoretic separation and illustrate that chemical accumulation at the subcellular level is highly heterogeneous. In individual mitochondria from cultured human leukemia cells DOX amount is around 50 zmol, 2 orders of magnitude higher than expected from diffusion during drug treatment, and spans 2 orders of magnitude.

Similar articles

• Selective determination of the doxorubicin content of individual acidic organelles in impure subcellular fractions.

  Chen Y, Walsh RJ, Arriaga EA. Chen Y, et al. Anal Chem. 2005 Apr 15;77(8):2281-7. doi: 10.1021/ac0480996. Anal Chem. 2005. PMID: 15828758

• Accelerator mass spectrometry allows for cellular quantification of doxorubicin at femtomolar concentrations.

  DeGregorio MW, Dingley KH, Wurz GT, Ubick E, Turteltaub KW. DeGregorio MW, et al. Cancer Chemother Pharmacol. 2006 Feb;57(3):335-42. doi: 10.1007/s00280-005-0060-1. Epub 2005 Jul 27. Cancer Chemother Pharmacol. 2006. PMID: 16047147

• [Lifetime quantitative assessment of intracellular distribution of doxorubicin in tumor cells].

  Bogush TA, Shubina IZh, Smirnova GB, Syrkin AB, Bogush EA. Bogush TA, et al. Biull Eksp Biol Med. 1995 Nov;120(11):518-21. Biull Eksp Biol Med. 1995. PMID: 8713333 Russian. No abstract available.

• A mechanistic study of enhanced doxorubicin uptake and retention in multidrug resistant breast cancer cells using a polymer-lipid hybrid nanoparticle system.

  Wong HL, Bendayan R, Rauth AM, Xue HY, Babakhanian K, Wu XY. Wong HL, et al. J Pharmacol Exp Ther. 2006 Jun;317(3):1372-81. doi: 10.1124/jpet.106.101154. Epub 2006 Mar 17. J Pharmacol Exp Ther. 2006. PMID: 16547167

• Analytical tools for characterizing heterogeneity in organelle content.

  Anand RK, Chiu DT. Anand RK, et al. Curr Opin Chem Biol. 2012 Aug;16(3-4):391-9. doi: 10.1016/j.cbpa.2012.05.187. Epub 2012 Jun 12. Curr Opin Chem Biol. 2012. PMID: 22694875 Free PMC article. Review.

Cited by

• Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton.

  Kostal V, Arriaga EA. Kostal V, et al. Anal Chem. 2011 Mar 1;83(5):1822-9. doi: 10.1021/ac200068p. Epub 2011 Feb 10. Anal Chem. 2011. PMID: 21309532 Free PMC article.

• Skeletal Muscle an Active Compartment in the Sequestering and Metabolism of Doxorubicin Chemotherapy.

  Fabris S, MacLean DA. Fabris S, et al. PLoS One. 2015 Sep 24;10(9):e0139070. doi: 10.1371/journal.pone.0139070. eCollection 2015. PLoS One. 2015. PMID: 26401619 Free PMC article.

• Mitochondrial ABC transporters function: the role of ABCB10 (ABC-me) as a novel player in cellular handling of reactive oxygen species.

  Liesa M, Qiu W, Shirihai OS. Liesa M, et al. Biochim Biophys Acta. 2012 Oct;1823(10):1945-57. doi: 10.1016/j.bbamcr.2012.07.013. Epub 2012 Aug 3. Biochim Biophys Acta. 2012. PMID: 22884976 Free PMC article. Review.

• Capillary electrophoresis monitors enhancement in subcellular reactive oxygen species production upon treatment with doxorubicin.

  Eder AR, Arriaga EA. Eder AR, et al. Chem Res Toxicol. 2006 Sep;19(9):1151-9. doi: 10.1021/tx060083i. Chem Res Toxicol. 2006. PMID: 16978019 Free PMC article.

• Doxorubicin Inhibits Phosphatidylserine Decarboxylase and Modifies Mitochondrial Membrane Composition in HeLa Cells.

  Bellance N, Furt F, Melser S, Lalou C, Thoraval D, Maneta-Peyret L, Lacombe D, Moreau P, Rossignol R. Bellance N, et al. Int J Mol Sci. 2020 Feb 15;21(4):1317. doi: 10.3390/ijms21041317. Int J Mol Sci. 2020. PMID: 32075281 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • American Chemical Society