Distribution and in situ proliferation patterns of intravenously injected immortalized human neural stem-like cells in rats with focal cerebral ischemia - PubMed

Distribution and in situ proliferation patterns of intravenously injected immortalized human neural stem-like cells in rats with focal cerebral ischemia

Kon Chu et al. Neurosci Res. 2004 Dec.

Abstract

Neural stem cells are considered as a candidate for cell replacement therapy in various neurological diseases. To investigate whether human neural stem cells can migrate into the adult ischemic rat brain, we transplanted immortalized human neural 'tem-like' cells intravenously 24 h after focal cerebral ischemia. The intravenously injected human neural stem-like cells were found around the infarcted area, differentiated into neurons and astrocytes in the lesioned areas, and survive up to 56 days after transplantation. The number of the injected cells increased between 7 and 14 days after transplantation with incorporating BrdU. Our findings show that intravenously injected human neural stem-like cells may incorporate into the ischemic brain, and undergo proliferation responding to the endogenous mitotic signal during the acute period of focal ischemia.

Similar articles

• Survival, migration and neuronal differentiation of human fetal striatal and cortical neural stem cells grafted in stroke-damaged rat striatum.

  Darsalia V, Kallur T, Kokaia Z. Darsalia V, et al. Eur J Neurosci. 2007 Aug;26(3):605-14. doi: 10.1111/j.1460-9568.2007.05702.x. Eur J Neurosci. 2007. PMID: 17686040

• Human neural stem cells improve sensorimotor deficits in the adult rat brain with experimental focal ischemia.

  Chu K, Kim M, Park KI, Jeong SW, Park HK, Jung KH, Lee ST, Kang L, Lee K, Park DK, Kim SU, Roh JK. Chu K, et al. Brain Res. 2004 Aug 6;1016(2):145-53. doi: 10.1016/j.brainres.2004.04.038. Brain Res. 2004. PMID: 15246850

• Comparison of ischemia-directed migration of neural precursor cells after intrastriatal, intraventricular, or intravenous transplantation in the rat.

  Jin K, Sun Y, Xie L, Mao XO, Childs J, Peel A, Logvinova A, Banwait S, Greenberg DA. Jin K, et al. Neurobiol Dis. 2005 Mar;18(2):366-74. doi: 10.1016/j.nbd.2004.10.010. Neurobiol Dis. 2005. PMID: 15686965

• [Therapeutic application of cell transplantation and increased neurogenesis in cerebral infarction].

  Kitagawa K. Kitagawa K. Rinsho Shinkeigaku. 2004 Nov;44(11):756-9. Rinsho Shinkeigaku. 2004. PMID: 15651283 Review. Japanese.

• [Brain ischemia--regenerative therapy using human neural stem cells].

  Mizusawa H. Mizusawa H. Rinsho Shinkeigaku. 2003 Nov;43(11):832-3. Rinsho Shinkeigaku. 2003. PMID: 15152478 Review. Japanese.

Cited by

• Immune influence on adult neural stem cell regulation and function.

  Carpentier PA, Palmer TD. Carpentier PA, et al. Neuron. 2009 Oct 15;64(1):79-92. doi: 10.1016/j.neuron.2009.08.038. Neuron. 2009. PMID: 19840551 Free PMC article. Review.

• Aesthetic cardiology: adipose-derived stem cells for myocardial repair.

  Palpant NJ, Metzger JM. Palpant NJ, et al. Curr Stem Cell Res Ther. 2010 Jun;5(2):145-52. doi: 10.2174/157488810791268654. Curr Stem Cell Res Ther. 2010. PMID: 19941452 Free PMC article. Review.

• Immunohistochemical toolkit for tracking and quantifying xenotransplanted human stem cells.

  Allard J, Li K, Lopez XM, Blanchard S, Barbot P, Rorive S, Decaestecker C, Pochet R, Bohl D, Lepore AC, Salmon I, Nicaise C. Allard J, et al. Regen Med. 2014;9(4):437-52. doi: 10.2217/rme.14.26. Regen Med. 2014. PMID: 25159062 Free PMC article.

• Stem cells' guided gene therapy of cancer: New frontier in personalized and targeted therapy.

  Mavroudi M, Zarogoulidis P, Porpodis K, Kioumis I, Lampaki S, Yarmus L, Malecki R, Zarogoulidis K, Malecki M. Mavroudi M, et al. J Cancer Res Ther (Manch). 2014;2(1):22-33. doi: 10.14312/2052-4994.2014-4. J Cancer Res Ther (Manch). 2014. PMID: 24860662 Free PMC article.

• Strategies for protecting oligodendrocytes and enhancing remyelination in multiple sclerosis.

  Rodgers JM, Robinson AP, Miller SD. Rodgers JM, et al. Discov Med. 2013 Aug;16(86):53-63. Discov Med. 2013. PMID: 23911232 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Medical

  • MedlinePlus Health Information