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Yersinia enterocolitica invasin-dependent and invasin-independent mechanisms of systemic dissemination - PubMed

Yersinia enterocolitica invasin-dependent and invasin-independent mechanisms of systemic dissemination

Scott A Handley et al. Infect Immun. 2005 Dec.

Abstract

We report here invasin-dependent and invasin-independent mechanisms in which the enteropathogen Yersinia enterocolitica is able to disseminate from the lumen of the small intestine to the spleen. The invasin-dependent route is clearly discernible in mice devoid of intestinal Peyer's patches and mesenteric lymph nodes.

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Figures

FIG. 1.
FIG. 1.

Colonization of C57BL/6J (▪) and LTα−/− (▵) mouse spleens following oral inoculation with (5 to 8) × 108 CFU wild-type Y. enterocolitica (JB580v). Infections were performed as described previously (10). At days 1, 3, and 7 postinfection, spleens were dissected and macerated into suspensions. Colonies were enumerated by plating serial dilutions of the suspensions onto Yersinia selective agar plates (Becton-Dickinson, Sparks, MD). The graph displays the combination of two independent experiments which used four to five mice per group per time point. The dashed line indicates the limit of detection for the assay.

FIG. 2.
FIG. 2.

Colonization of C57BL/6J (▪) and LTα−/− (▵) mouse spleens following oral inoculation with (5 to 8) × 108 CFU of inv mutant Y. enterocolitica (JP273v). Infections were performed as described previously (10). Spleen colonization was assessed as described in the legend to Fig. 1. The graph displays the combination of two independent experiments which used five mice per time point. The dashed line indicates the limit of detection for the assay.

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