The human SWI/SNF subunit Brm is a regulator of alternative splicing - PubMed

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• PMID: 16341228
• DOI: 10.1038/nsmb1030

The human SWI/SNF subunit Brm is a regulator of alternative splicing

Eric Batsché et al. Nat Struct Mol Biol. 2006 Jan.

Abstract

The SWI/SNF (mating-type switch/sucrose nonfermenting) complex involved in chromatin remodeling on promoters has also been detected on the coding region of genes. Here we show that SWI/SNF can function as a regulator of alternative splicing. We found that the catalytic subunit Brm favors inclusion of variant exons in the mRNA of several genes, including E-cadherin, BIM, cyclin D1 and CD44. Consistent with this, Brm associates with several components of the spliceosome and with Sam68, an ERK-activated enhancer of variant exon inclusion. Examination of the CD44 gene revealed that Brm induced accumulation of RNA polymerase II (RNAPII) with a modified CTD phosphorylation pattern on regions encoding variant exons. Altogether, our data suggest that on genes regulated by SWI/SNF, Brm contributes to the crosstalk between transcription and RNA processing by decreasing RNAPII elongation rate and facilitating recruitment of the splicing machinery to variant exons with suboptimal splice sites.

Comment in

• Chromatin, transcript elongation and alternative splicing.

  Kornblihtt AR. Kornblihtt AR. Nat Struct Mol Biol. 2006 Jan;13(1):5-7. doi: 10.1038/nsmb0106-5. Nat Struct Mol Biol. 2006. PMID: 16395314

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