The small GTP-binding protein rac regulates growth factor-induced membrane ruffling - PubMed

The small GTP-binding protein rac regulates growth factor-induced membrane ruffling

A J Ridley et al. Cell. 1992.

Abstract

The function of rac, a ras-related GTP-binding protein, was investigated in fibroblasts by microinjection. In confluent serum-starved Swiss 3T3 cells, rac1 rapidly stimulated actin filament accumulation at the plasma membrane, forming membrane ruffles. Several growth factors and activated H-ras also induced membrane ruffling, and this response was prevented by a dominant inhibitory mutant rac protein, N17rac1. This suggests that endogenous rac proteins are required for growth factor-induced membrane ruffling. In addition to membrane ruffling, a later response to both rac1 microinjection and some growth factors was the formation of actin stress fibers, a process requiring endogenous rho proteins. Using N17rac1 we have shown that these growth factors act through rac to stimulate this rho-dependent response. We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin.

Comment in

• Rho and Rac take center stage.

  Burridge K, Wennerberg K. Burridge K, et al. Cell. 2004 Jan 23;116(2):167-79. doi: 10.1016/s0092-8674(04)00003-0. Cell. 2004. PMID: 14744429 Review.

• Snails, Swiss, and serum: the solution for Rac 'n' Rho.

  Ridley AJ, Hall A. Ridley AJ, et al. Cell. 2004 Jan 23;116(2 Suppl):S23-5, 2 p following S25. doi: 10.1016/s0092-8674(04)00048-0. Cell. 2004. PMID: 15055577 No abstract available.

• How a paper on RAC set the standard.

  Insall R. Insall R. Nat Rev Mol Cell Biol. 2012 Jan 23;13(2):66. doi: 10.1038/nrm3277. Nat Rev Mol Cell Biol. 2012. PMID: 22266759 No abstract available.

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