Localization of carbamoylphosphate synthetase and aspartate carbamoyltransferase in chloroplasts - PubMed

Localization of carbamoylphosphate synthetase and aspartate carbamoyltransferase in chloroplasts

H Shibata et al. Plant Physiol. 1986 Jan.

Abstract

The localization of carbamoylphosphate synthetase (CPSase) and aspartate carbamoyltransferase (ACTase), the first two enzymes of the pyrimidine biosynthetic pathway, in chloroplasts was investigated. In dark-grown radish (Raphanus sativus) seedlings, light induced a prominent increase in CPSase activity, but had little effect on ACTase activity. Both enzymes were found in chloroplasts isolated from radish cotyledons and leaves of spinach (Spinacia oleracea), soybean (Glycine max), and corn (Zea mays). The higher activity of ACTase relative to CPSase is discussed in relation to the instability of carbamoylphosphate, the product of the CPSase, and to the control of pyrimidine synthesis. Based on these results, the function of CPSase and ACTase in chloroplasts is discussed.

Similar articles

• Pyrimidine biosynthesis in Neisseria meningitidis. 2. Regulation of enzyme synthesis.

  Jyssum S. Jyssum S. Acta Pathol Microbiol Immunol Scand B. 1983 Aug;91(4):257-60. doi: 10.1111/j.1699-0463.1983.tb00043.x. Acta Pathol Microbiol Immunol Scand B. 1983. PMID: 6414243

• Pyrimidine biosynthesis in Neisseria meningitidis. 1. Demonstration of enzyme activities.

  Jyssum S. Jyssum S. Acta Pathol Microbiol Immunol Scand B. 1983 Aug;91(4):251-5. doi: 10.1111/j.1699-0463.1983.tb00042.x. Acta Pathol Microbiol Immunol Scand B. 1983. PMID: 6137930

• Mapping of catalytic domains and phosphorylation sites in the multifunctional pyrimidine-biosynthetic protein CAD.

  Carrey EA, Hardie DG. Carrey EA, et al. Eur J Biochem. 1988 Feb 1;171(3):583-8. doi: 10.1111/j.1432-1033.1988.tb13828.x. Eur J Biochem. 1988. PMID: 3345746

Cited by

• Molecular cloning and characterization of the pyrB1 and pyrB2 genes encoding aspartate transcarbamoylase in pea (Pisum sativum L.).

  Williamson CL, Slocum RD. Williamson CL, et al. Plant Physiol. 1994 May;105(1):377-84. doi: 10.1104/pp.105.1.377. Plant Physiol. 1994. PMID: 8029359 Free PMC article.

• Purine and pyrimidine nucleotide synthesis and metabolism.

  Moffatt BA, Ashihara H. Moffatt BA, et al. Arabidopsis Book. 2002;1:e0018. doi: 10.1199/tab.0018. Epub 2002 Apr 4. Arabidopsis Book. 2002. PMID: 22303196 Free PMC article. No abstract available.

• Citrulline and DRIP-1 protein (ArgE homologue) in drought tolerance of wild watermelon.

  Yokota A, Kawasaki S, Iwano M, Nakamura C, Miyake C, Akashi K. Yokota A, et al. Ann Bot. 2002 Jun;89 Spec No(7):825-32. doi: 10.1093/aob/mcf074. Ann Bot. 2002. PMID: 12102508 Free PMC article. Review.

• Partial characterization of ornithine carbamoyltransferase in three microalgae : anabolic role only.

  Laliberté G, Hellebust JA. Laliberté G, et al. Plant Physiol. 1990 May;93(1):62-6. doi: 10.1104/pp.93.1.62. Plant Physiol. 1990. PMID: 16667466 Free PMC article.

• Arabidopsis chloroplasts dissimilate L-arginine and L-citrulline for use as N source.

  Ludwig RA. Ludwig RA. Plant Physiol. 1993 Feb;101(2):429-34. doi: 10.1104/pp.101.2.429. Plant Physiol. 1993. PMID: 8278506 Free PMC article.

References

LinkOut - more resources

• Full Text Sources

  • PubMed Central
  • Silverchair Information Systems