Induction of gynogenesis in Japanese crucian carp (Carassius cuvieri) - PubMed

Induction of gynogenesis in Japanese crucian carp (Carassius cuvieri)

Yuan-Dong Sun et al. Yi Chuan Xue Bao. 2006 May.

Abstract

Diploid gynogenesis was induced in Japanese crucian carp (Carassius cuvieri) eggs using UV-irradiated genetically inactive spermatozoa from mirror carp (Cyprinus carpio L.) or blunt snout bream (Megalobrama amblycephala), with or without cold shock. The optimal radiation dosage was 4,200 mJ/cm2 and 3,600 mJ/cm2 for mirror carp and blunt snout bream sperm, respectively. At this dosage and without cold shock, the yields were (32.4 +/- 3.3)% vs. (33.8 +/- 1.4)% gynogenetic haploids and (0.7 +/- 0.3)% vs. (0.5 +/- 0.3)% hybrid diploids, respectively. At the optimal UV dosage but with cold shock (2 min after fertilization, 0-4 degrees C for 40 min), the hatching rates were (27.8 +/- 2.1)% and (29.4 +/- 3.3)%, respectively. From hatching to feeding, (15.7 +/- 3.4)% and (23.6 +/- 4.1)% normal gynogenetic diploids were recorded, respectively. Survival of normal gynogenetic diploids was 56% out of the hatched fry when using irradiated spermatozoa of mirror carp, which was lower than that (up to 80%) when using irradiated spermatozoa of blunt snout bream. This indicated that the sperm of blunt snout bream, with distant genetic relation to the maternal Japanese crucian carp, was more effective than that of mirror carp to induce diploid gynogenesis. The nature of the gynogenetic progeny was identified with external appearance, chromosome number and gonad structure. The presence of only females in gynogenetic progeny probably suggested XX genotype in the female Japanese crucian carp. The gynogenetic diploids have potential values such as faster growth and stronger disease resistance than the normal Japanese crucian carp. All gynogenetic progeny possessed 100 chromosomes whereas all J x B crosses were triploid with 124 chromosomes. The formation of the new triploid hybrids in J x B crosses may be useful in aquaculture.

Similar articles

• [Comparative studies on gene expression of isozymes in 4 types of various ploidy crucian carp during embryogenesis].

  Zhu LF, Gui JF. Zhu LF, et al. Shi Yan Sheng Wu Xue Bao. 1998 Dec;31(4):369-76. Shi Yan Sheng Wu Xue Bao. 1998. PMID: 12016959 Chinese.

• Microsatellite analysis of different ploidy offspring of artificial gynogenesis in Cyprinus carpio.

  Xiao J, Zou TM, Chen L, Liu SJ, Xiao J, Zhang H, Long Y, Yan JP, Zhao RR, Tao M, Zhang C, You CP, Liu Y. Xiao J, et al. J Fish Biol. 2011 Jan;78(1):150-65. doi: 10.1111/j.1095-8649.2010.02848.x. Epub 2010 Dec 14. J Fish Biol. 2011. PMID: 21235552

• Distant hybridization leads to different ploidy fishes.

  Liu S. Liu S. Sci China Life Sci. 2010 Apr;53(4):416-25. doi: 10.1007/s11427-010-0057-9. Epub 2010 May 7. Sci China Life Sci. 2010. PMID: 20596907 Review.

• Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp x common carp.

  Liu S, Duan W, Tao M, Zhang C, Sun Y, Shen J, Wang J, Luo K, Liu Y. Liu S, et al. Sci China C Life Sci. 2007 Apr;50(2):186-93. doi: 10.1007/s11427-007-0032-2. Sci China C Life Sci. 2007. PMID: 17447025

• [Gynogenesis in vertebrates].

  Chourrout D. Chourrout D. Reprod Nutr Dev (1980). 1982;22(5):713-34. Reprod Nutr Dev (1980). 1982. PMID: 6760302 Review. French.

Cited by

• Induction of diploid gynogenesis in an evolutionary model organism, the three-spined stickleback (Gasterosteus aculeatus).

  Samonte-Padilla IE, Eizaguirre C, Scharsack JP, Lenz TL, Milinski M. Samonte-Padilla IE, et al. BMC Dev Biol. 2011 Sep 12;11:55. doi: 10.1186/1471-213X-11-55. BMC Dev Biol. 2011. PMID: 21910888 Free PMC article.

• Massive production of all-female diploids and triploids in the crucian carp.

  Luo K, Xiao J, Liu S, Wang J, He W, Hu J, Qin Q, Zhang C, Tao M, Liu Y. Luo K, et al. Int J Biol Sci. 2011 Apr 16;7(4):487-95. doi: 10.7150/ijbs.7.487. Int J Biol Sci. 2011. PMID: 21547066 Free PMC article.

• Evidence for the formation of the male gynogenetic fish.

  Liu S, Qin Q, Wang Y, Zhang H, Zhao R, Zhang C, Wang J, Li W, Chen L, Xiao J, Luo K, Tao M, Duan W, Liu Y. Liu S, et al. Mar Biotechnol (NY). 2010 Apr;12(2):160-72. doi: 10.1007/s10126-009-9219-9. Epub 2009 Jul 15. Mar Biotechnol (NY). 2010. PMID: 19603230

• Induced gynogenesis in grass carp (Ctenopharyngodon idellus) using irradiated sperm of allotetraploid hybrids.

  Zhang H, Liu S, Zhang C, Tao M, Peng L, You C, Xiao J, Zhou Y, Zhou G, Luo K, Liu Y. Zhang H, et al. Mar Biotechnol (NY). 2011 Oct;13(5):1017-26. doi: 10.1007/s10126-011-9365-8. Epub 2011 Jan 29. Mar Biotechnol (NY). 2011. PMID: 21279407

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Research Materials

  • NCI CPTC Antibody Characterization Program