Printing your own inkjet microarrays - PubMed

Review

Printing your own inkjet microarrays

Christopher G Lausted et al. Methods Enzymol. 2006.

Abstract

DNA arrays are now the tools of choice for high-throughput DNA/RNA analysis. While many technologies exist for mass-producing arrays, there are just a few ways to economically produce small batches of custom oligonucleotide arrays for prototyping experiments and specialized applications. Inkjet printing, adapted from the world of office electronics to the world of molecular biology, is one such method. With programmable oligonucleotide synthesizers, scientists can prototype DNA array assays quickly and inexpensively. A benchtop inkjet arrayer-nicknamed POSAM-can be built by most skilled molecular biology laboratories. Inkjet arrays can fulfill the changing needs of those studying the complex network of relationships in systems biology.

Similar articles

• POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer.

  Lausted C, Dahl T, Warren C, King K, Smith K, Johnson M, Saleem R, Aitchison J, Hood L, Lasky SR. Lausted C, et al. Genome Biol. 2004;5(8):R58. doi: 10.1186/gb-2004-5-8-r58. Epub 2004 Jul 27. Genome Biol. 2004. PMID: 15287980 Free PMC article.

• Quality control of inkjet technology for DNA microarray fabrication.

  Pierik A, Dijksman F, Raaijmakers A, Wismans T, Stapert H. Pierik A, et al. Biotechnol J. 2008 Dec;3(12):1581-90. doi: 10.1002/biot.200800121. Biotechnol J. 2008. PMID: 19039779

• The printing process: tips on tips.

  George RA. George RA. Methods Enzymol. 2006;410:121-35. doi: 10.1016/S0076-6879(06)10006-3. Methods Enzymol. 2006. PMID: 16938549 Review.

• Printing protein arrays from DNA arrays.

  He M, Stoevesandt O, Palmer EA, Khan F, Ericsson O, Taussig MJ. He M, et al. Nat Methods. 2008 Feb;5(2):175-7. doi: 10.1038/nmeth.1178. Epub 2008 Jan 20. Nat Methods. 2008. PMID: 18204456

• Microarray-based DNA profiling to study genomic aberrations.

  Waddell N. Waddell N. IUBMB Life. 2008 Jul;60(7):437-40. doi: 10.1002/iub.57. IUBMB Life. 2008. PMID: 18553550 Review.

Cited by

• Microscale patterning of thermoplastic polymer surfaces by selective solvent swelling.

  Rahmanian O, Chen CF, DeVoe DL. Rahmanian O, et al. Langmuir. 2012 Sep 4;28(35):12923-9. doi: 10.1021/la302704t. Epub 2012 Aug 23. Langmuir. 2012. PMID: 22900539 Free PMC article.

• Accessing individual 75-micron diameter nozzles of a desktop inkjet printer to dispense picoliter droplets on demand.

  Waasdorp R, van den Heuvel O, Versluis F, Hajee B, Ghatkesar MK. Waasdorp R, et al. RSC Adv. 2018 Apr 18;8(27):14765-14774. doi: 10.1039/c8ra00756j. eCollection 2018 Apr 18. RSC Adv. 2018. PMID: 35541355 Free PMC article.

• Multiplexed protein analysis using encoded antibody-conjugated microbeads.

  Theilacker N, Roller EE, Barbee KD, Franzreb M, Huang X. Theilacker N, et al. J R Soc Interface. 2011 Aug 7;8(61):1104-13. doi: 10.1098/rsif.2010.0594. Epub 2011 Jan 19. J R Soc Interface. 2011. PMID: 21247950 Free PMC article.

• Pen microfluidics: rapid desktop manufacturing of sealed thermoplastic microchannels.

  Rahmanian O, DeVoe DL. Rahmanian O, et al. Lab Chip. 2013 Mar 21;13(6):1102-8. doi: 10.1039/c2lc41057e. Lab Chip. 2013. PMID: 23344819 Free PMC article.

• Protein Microarrays with Novel Microfluidic Methods: Current Advances.

  Dixit CK, Aguirre GR. Dixit CK, et al. Microarrays (Basel). 2014 Jul 1;3(3):180-202. doi: 10.3390/microarrays3030180. Microarrays (Basel). 2014. PMID: 27600343 Free PMC article. Review.

Publication types

MeSH terms