The influence of nuclear content on developmental competence of gaur x cattle hybrid in vitro fertilized and somatic cell nuclear transfer embryos - PubMed

The influence of nuclear content on developmental competence of gaur x cattle hybrid in vitro fertilized and somatic cell nuclear transfer embryos

Gabriela F Mastromonaco et al. Biol Reprod. 2007 Mar.

Abstract

In nondomestic and endangered species, the use of domestic animal oocytes as recipients for exotic donor nuclei causes the normal pattern of cytoplasmic inheritance to be disrupted, resulting in the production of nuclear-cytoplasmic hybrids. Evidence suggests that conflict between nuclear and cytoplasmic control elements leads to a disruption of normal cellular processes, including metabolic function and cell division. This study investigated the effects of nuclear-cytoplasmic interactions on the developmental potential of interspecies embryos produced by in vitro fertilization and somatic cell nuclear transfer: cattle x cattle, gaur x cattle, hybrid x cattle. Cattle control and hybrid embryos were examined for development to the blastocyst stage and blastocyst quality, as determined by cell number and allocation, apoptosis incidence, and expression patterns of mitochondria-related genes. These analyses demonstrated that a 100% gaur nucleus within a domestic cattle cytoplasmic environment was not properly capable of directing embryo development in the later preimplantation stages. Poor blastocyst development accompanied by developmental delay, decreased cell numbers, and aberrant apoptotic and related gene expression profiles, all signs of disrupted cellular processes associated with mitochondrial function, were observed. Developmental potential was improved when at least a portion of the nuclear genome corresponded to the inherited cytoplasm, indicating that recognition of cytoplasmic components by the nucleus is crucial for proper cellular function and embryo development. A better understanding of the influence of the cytoplasmic environment on embryonic processes is necessary before interspecies somatic cell nuclear transfer can be considered a viable alternative for endangered species conservation.

Similar articles

• Nuclear-cytoplasmic incompatibility and inefficient development of pig-mouse cytoplasmic hybrid embryos.

  Amarnath D, Choi I, Moawad AR, Wakayama T, Campbell KH. Amarnath D, et al. Reproduction. 2011 Aug;142(2):295-307. doi: 10.1530/REP-11-0044. Epub 2011 May 9. Reproduction. 2011. PMID: 21555359

• Ooplasm transfer and interspecies somatic cell nuclear transfer: heteroplasmy, pattern of mitochondrial migration and effect on embryo development.

  Sansinena MJ, Lynn J, Bondioli KR, Denniston RS, Godke RA. Sansinena MJ, et al. Zygote. 2011 May;19(2):147-56. doi: 10.1017/S0967199410000419. Epub 2010 Aug 25. Zygote. 2011. PMID: 20735895

• Gene expression and development of early pig embryos produced by serial nuclear transfer.

  Xing X, Magnani L, Lee K, Wang C, Cabot RA, Machaty Z. Xing X, et al. Mol Reprod Dev. 2009 Jun;76(6):555-63. doi: 10.1002/mrd.20974. Mol Reprod Dev. 2009. PMID: 18951379

• Nuclear transfer in cats and its application.

  Gómez MC, Pope CE, Dresser BL. Gómez MC, et al. Theriogenology. 2006 Jul 1;66(1):72-81. doi: 10.1016/j.theriogenology.2006.03.017. Epub 2006 Apr 18. Theriogenology. 2006. PMID: 16620927 Review.

• Nuclear transfer: preservation of a nuclear genome at the expense of its associated mtDNA genome(s).

  Bowles EJ, Campbell KH, St John JC. Bowles EJ, et al. Curr Top Dev Biol. 2007;77:251-90. doi: 10.1016/S0070-2153(06)77010-7. Curr Top Dev Biol. 2007. PMID: 17222707 Review.

Cited by

• A newly developed cloning technique in sturgeons; an important step towards recovering endangered species.

  Fatira E, Havelka M, Labbé C, Depincé A, Pšenička M, Saito T. Fatira E, et al. Sci Rep. 2019 Jul 18;9(1):10453. doi: 10.1038/s41598-019-46892-4. Sci Rep. 2019. PMID: 31320687 Free PMC article.

• Conservation Mitonuclear Replacement: Facilitated mitochondrial adaptation for a changing world.

  Iverson ENK. Iverson ENK. Evol Appl. 2024 Mar 10;17(3):e13642. doi: 10.1111/eva.13642. eCollection 2024 Mar. Evol Appl. 2024. PMID: 38468713 Free PMC article.

• Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes.

  Lee E, Bhuiyan MM, Watanabe H, Matsuoka K, Fujise Y, Ishikawa H, Fukui Y. Lee E, et al. J Vet Sci. 2009 Dec;10(4):285-92. doi: 10.4142/jvs.2009.10.4.285. J Vet Sci. 2009. PMID: 19934592 Free PMC article.

• Mitochondrial DNA transmission and confounding mitochondrial influences in cloned cattle and pigs.

  Takeda K. Takeda K. Reprod Med Biol. 2013 Jan 10;12(2):47-55. doi: 10.1007/s12522-012-0142-9. eCollection 2013 Apr. Reprod Med Biol. 2013. PMID: 29699130 Free PMC article. Review.

• In vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls.

  Revay T, Oluwole O, Kroetsch T, King WA. Revay T, et al. Sci Rep. 2017 May 9;7(1):1631. doi: 10.1038/s41598-017-01793-2. Sci Rep. 2017. PMID: 28487564 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Silverchair Information Systems