Endothelin modulates L-NG-nitroarginine-induced enhancement of vasoconstriction evoked by norepinephrine - PubMed

Endothelin modulates L-NG-nitroarginine-induced enhancement of vasoconstriction evoked by norepinephrine

Y Tabuchi et al. J Cardiovasc Pharmacol. 1991.

Abstract

The interaction of endothelin-1 (ET-1), a novel vasoconstrictor peptide synthesized according to the encoded amino acid sequence, with L-NG-nitroarginine (NOARG), an L-arginine-reversible inhibitor of endothelium-derived relaxing factor (EDRF) on adrenergic receptors, was investigated. Male Sprague-Dawley rats were used in this study. Mesenteric arteries were isolated and perfused with Krebs-Ringer solution at a constant flow rate. The vasoconstrictor responses to exogenous norepinephrine were determined. Low concentrations of ET-1 (10(-11) and 10(-10) M) potentiated the pressor responses to norepinephrine without affecting the baseline perfusion pressure. Simultaneous NOARG (10(-5) M) infusion with ET-1 (10(-11) M) into the mesenteric arteries caused further enhancement of the pressor responses to exogenous norepinephrine (200 ng) without affecting the baseline perfusion pressure: 126 +/- 15% to 262 +/- 45% (p less than 0.05, vehicle control: 100%). This facilitatory effect of NOARG on the pressor response to norepinephrine was attenuated with L-arginine (10(-4) M) infusion, but not D-arginine. These results suggest that ET, one of the endothelium-derived vasoconstricting factors, interacts with EDRF to modulate the tone of resistance vessels.

Similar articles

• Endothelium-dependent vascular activities of endothelin-like peptides in the isolated superior mesenteric arterial bed of the rat.

  Douglas SA, Hiley CR. Douglas SA, et al. Br J Pharmacol. 1990 Sep;101(1):81-8. doi: 10.1111/j.1476-5381.1990.tb12093.x. Br J Pharmacol. 1990. PMID: 2282471 Free PMC article.

• Modulation of norepinephrine-induced vasoconstriction by endothelin-1 and nitric oxide in rat tail artery.

  Reid JJ, Vo PA, Lieu AT, Wong-Dusting HK, Rand MJ. Reid JJ, et al. J Cardiovasc Pharmacol. 1991;17 Suppl 7:S272-5. doi: 10.1097/00005344-199100177-00077. J Cardiovasc Pharmacol. 1991. PMID: 1725353

• Endothelial modulation and changes in endothelin pressor activity during hypoxia in the rat isolated perfused superior mesenteric arterial bed.

  Douglas SA, James S, Hiley CR. Douglas SA, et al. Br J Pharmacol. 1991 Jun;103(2):1441-8. doi: 10.1111/j.1476-5381.1991.tb09808.x. Br J Pharmacol. 1991. PMID: 1884099 Free PMC article.

• The effects of perfusion rate and NG-nitro-L-arginine methyl ester on cirazoline- and KCl-induced responses in the perfused mesenteric arterial bed of rats.

  Adeagbo AS, Tabrizchi R, Triggle CR. Adeagbo AS, et al. Br J Pharmacol. 1994 Jan;111(1):13-20. doi: 10.1111/j.1476-5381.1994.tb14017.x. Br J Pharmacol. 1994. PMID: 7912152 Free PMC article.

• Increased sensitivity to endothelin-1 in isolated Krebs'-perfused kidneys of streptozotocin-diabetic rats.

  Tammesild PJ, Hodgson WC, King RG. Tammesild PJ, et al. Clin Exp Pharmacol Physiol. 1992 Apr;19(4):261-5. doi: 10.1111/j.1440-1681.1992.tb00448.x. Clin Exp Pharmacol Physiol. 1992. PMID: 1516274

Cited by

• Endothelin alters the reactivity of vasa vasorum: mechanisms and implications for conduit vessel physiology and pathophysiology.

  Scotland R, Vallance P, Ahluwalia A. Scotland R, et al. Br J Pharmacol. 1999 Nov;128(6):1229-34. doi: 10.1038/sj.bjp.0702930. Br J Pharmacol. 1999. PMID: 10578136 Free PMC article.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.

• Miscellaneous

  • NCI CPTAC Assay Portal