Biotechnological production of spider-silk proteins enables new applications - PubMed

Affiliations

• PMID: 17429812
• DOI: 10.1002/mabi.200600255

Review

Biotechnological production of spider-silk proteins enables new applications

Charlotte Vendrely et al. Macromol Biosci. 2007.

Abstract

The outstanding mechanical properties of spider silks have motivated many researchers to establish biotechnological production techniques which are necessary to provide sufficient amounts of silk proteins for industrial applications. Based on recent developments in genetic engineering, two strategies for the recombinant production of spider-silk proteins have been established which are discussed in detail. Further, protein-design strategies are described, enabling the combination of silk properties with additional biological, chemical, or technical features. We highlight the potential of engineered and recombinantly-produced spider-silk proteins to provide the basis for a new generation of biomaterials.

Similar articles

• Spider silks and their applications.

  Kluge JA, Rabotyagova O, Leisk GG, Kaplan DL. Kluge JA, et al. Trends Biotechnol. 2008 May;26(5):244-51. doi: 10.1016/j.tibtech.2008.02.006. Epub 2008 Mar 25. Trends Biotechnol. 2008. PMID: 18367277 Review.

• Recombinant spider silk proteins for applications in biomaterials.

  Spiess K, Lammel A, Scheibel T. Spiess K, et al. Macromol Biosci. 2010 Sep 9;10(9):998-1007. doi: 10.1002/mabi.201000071. Macromol Biosci. 2010. PMID: 20602494 Review.

• De Novo Design of Recombinant Spider Silk Proteins for Material Applications.

  Zheng K, Ling S. Zheng K, et al. Biotechnol J. 2019 Jan;14(1):e1700753. doi: 10.1002/biot.201700753. Epub 2018 Jun 5. Biotechnol J. 2019. PMID: 29781251 Review.

• A novel model system for design of biomaterials based on recombinant analogs of spider silk proteins.

  Bogush VG, Sokolova OS, Davydova LI, Klinov DV, Sidoruk KV, Esipova NG, Neretina TV, Orchanskyi IA, Makeev VY, Tumanyan VG, Shaitan KV, Debabov VG, Kirpichnikov MP. Bogush VG, et al. J Neuroimmune Pharmacol. 2009 Mar;4(1):17-27. doi: 10.1007/s11481-008-9129-z. Epub 2008 Oct 7. J Neuroimmune Pharmacol. 2009. PMID: 18839314

• Nanostructured, Self-Assembled Spider Silk Materials for Biomedical Applications.

  Humenik M, Pawar K, Scheibel T. Humenik M, et al. Adv Exp Med Biol. 2019;1174:187-221. doi: 10.1007/978-981-13-9791-2_6. Adv Exp Med Biol. 2019. PMID: 31713200 Review.

Cited by

• Purification and cytotoxicity of tag-free bioengineered spider silk proteins.

  Dams-Kozlowska H, Majer A, Tomasiewicz P, Lozinska J, Kaplan DL, Mackiewicz A. Dams-Kozlowska H, et al. J Biomed Mater Res A. 2013 Feb;101(2):456-64. doi: 10.1002/jbm.a.34353. Epub 2012 Aug 3. J Biomed Mater Res A. 2013. PMID: 22865581 Free PMC article.

• Spider Silk for Tissue Engineering Applications.

  Salehi S, Koeck K, Scheibel T. Salehi S, et al. Molecules. 2020 Feb 8;25(3):737. doi: 10.3390/molecules25030737. Molecules. 2020. PMID: 32046280 Free PMC article. Review.

• Spinning gland transcriptomics from two main clades of spiders (order: Araneae)--insights on their molecular, anatomical and behavioral evolution.

  Prosdocimi F, Bittencourt D, da Silva FR, Kirst M, Motta PC, Rech EL. Prosdocimi F, et al. PLoS One. 2011;6(6):e21634. doi: 10.1371/journal.pone.0021634. Epub 2011 Jun 29. PLoS One. 2011. PMID: 21738742 Free PMC article.

• Interfacial Crystallization and Supramolecular Self-Assembly of Spider Silk Inspired Protein at the Water-Air Interface.

  Mohammadi P, Zemke F, Wagermaier W, Linder MB. Mohammadi P, et al. Materials (Basel). 2021 Jul 29;14(15):4239. doi: 10.3390/ma14154239. Materials (Basel). 2021. PMID: 34361434 Free PMC article.

• Sequence Identification, Recombinant Production, and Analysis of the Self-Assembly of Egg Stalk Silk Proteins from Lacewing Chrysoperla carnea.

  Neuenfeldt M, Scheibel T. Neuenfeldt M, et al. Biomolecules. 2017 Jun 13;7(2):43. doi: 10.3390/biom7020043. Biomolecules. 2017. PMID: 28608840 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database