Induced pluripotent stem cell lines derived from human somatic cells - PubMed

Affiliations

• PMID: 18029452
• DOI: 10.1126/science.1151526

Induced pluripotent stem cell lines derived from human somatic cells

Junying Yu et al. Science. 2007.

Abstract

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.

Comment in

• Stem cell breakthrough: don't forget ethics.

  Lanza R. Lanza R. Science. 2007 Dec 21;318(5858):1865. doi: 10.1126/science.318.5858.1865a. Science. 2007. PMID: 18096789 No abstract available.

• Development. Is therapeutic cloning dead?

  Cibelli J. Cibelli J. Science. 2007 Dec 21;318(5858):1879-80. doi: 10.1126/science.1153229. Science. 2007. PMID: 18096796 No abstract available.

• Induced pluripotent cells mimicking human embryonic stem cells.

  Mohamadnejad M, Swenson ES. Mohamadnejad M, et al. Arch Iran Med. 2008 Jan;11(1):125-8. Arch Iran Med. 2008. PMID: 18154436 No abstract available.

• Achieving pluripotency.

  Baumann K. Baumann K. Nat Rev Mol Cell Biol. 2010 Oct;11(10):677. doi: 10.1038/nrm2980. Nat Rev Mol Cell Biol. 2010. PMID: 20861870 No abstract available.

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