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Telithromycin and quinupristin-dalfopristin induce delayed death in Plasmodium falciparum - PubMed

Telithromycin and quinupristin-dalfopristin induce delayed death in Plasmodium falciparum

Diana Barthel et al. Antimicrob Agents Chemother. 2008 Feb.

Abstract

Antibacterial agents are used in malaria therapy due to their effect on two prokaryote organelles, the mitochondrion and the apicoplast. We demonstrate here that the ribosome-blocking antibiotics telithromycin and quinupristin-dalfopristin, but not linezolid, inhibit the growth of Plasmodium falciparum. Both drugs induce delayed death in the parasite, suggesting that their effect involves the impairment of apicoplast translation processes.

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Figures

**FIG. 1.**
Antibiotic treatment causes developmental arrest and morphological abnormalities during the second replication cycle. (A) Histogram indicating parasite stages during drug treatment after different incubation times compared to an untreated control. Synchronized parasites were incubated with the antibiotics at the respective IC₅₀s or with 0.5% DMSO by volume (untreated control) over three life cycles. Giemsa smears were made at different time points, and the ring stages, trophozoites, early schizonts, and mature schizonts were counted. (B) Transmission electron micrographs revealing antibiotic-induced ultrastructural abnormalities after developmental arrest at 96 h of assay. HZ, hemozoin; M, merozoite; N, nucleus; V, vacuole. Bar, 1 μm.

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Telithromycin and quinupristin-dalfopristin induce delayed death in Plasmodium falciparum - PubMed